PDE1C (NM_001191056) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	cam-PDE 1C; DFNA74; hCam-3; Hcam3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233033 representing NM_001191056 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGCCAACCAAGGAGATTGAAGAATTTGAGAGCAACTCTCTGAAATACCTGCAACCGGAACAGA TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGATTACGGTCTTT GGTCAAACAATTAGAGAGAGGGGAAGCTTCAGTGGTAGATCTTAAGAAGAATTTGGAATATGCAGCCACA GTGCTTGAATCTGTGTATATTGATGAAACAAGGAGACTCCTGGATACAGAGGATGAGCTCAGTGACATTC AGTCAGATGCTGTGCCTTCTGAGGTCCGAGACTGGCTGGCCTCCACCTTCACGCGGCAGATGGGGATGAT GCTCAGGAGGAGCGACGAGAAGCCCCGGTTCAAGAGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTG GAGAGAATGTATAGACGGACATCAAACATGGTTGGACTGAGCTATCCACCAGCTGTTATTGAGGCATTAA AGGATGTGGACAAGTGGTCCTTTGACGTCTTTTCCCTCAATGAGGCCAGTGGGGATCATGCACTGAAATT TATTTTCTATGAACTACTCACACGTTATGATCTGATCAGCCGTTTCAAGATCCCCATTTCTGCACTTGTC TCATTTGTGGAGGCCCTGGAAGTGGGATACAGCAAGCACAAAAATCCTTACCATAACTTAATGCACGCTG CCGATGTTACACAGACAGTGCATTACCTCCTCTATAAGACAGGAGTGGCGAACTGGCTGACGGAGCTGGA GATCTTTGCTATAATCTTCTCAGCTGCCATCCATGACTACGAGCATACCGGAACCACCAACAATTTCCAC ATTCAGACTCGGTCTGATCCAGCTATTCTGTATAATGACAGATCTGTACTGGAGAATCACCATTTAAGTG CAGCTTATCGCCTTCTGCAAGATGACGAGGAAATGAATATTTTGATTAACCTCTCAAAGGATGACTGGAG GGAGTTTCGAACCTTGGTAATTGAAATGGTGATGGCCACAGATATGTCTTGTCACTTCCAACAAATCAAA GCAATGAAGACTGCTCTGCAGCAGCCAGAAGCCATTGAAAAGCCAAAAGCCTTATCCCTTATGCTGCATA CAGCAGATATTAGCCATCCAGCAAAAGCATGGGACCTCCATCATCGCTGGACAATGTCACTCCTGGAGGA GTTCTTCAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCTTTTTCTCCTCTGTGTGACCGAAAGTCC ACTATGGTTGCTCAGTCACAAGTAGGTTTCATTGATTTCATCGTGGAACCCACCTTCACTGTGCTTACGG ACATGACCGAGAAGATTGTGAGTCCATTAATCGATGAAACCTCTCAAACTGGTGGGACAGGACAGAGGCG TTCGAGTTTGAATAGCATCAGCTCGTCAGATGCCAAGCGATCAGGTGTCAAGACCTCTGGTTCAGAGGGA AGTGCCCCGATCAACAATTCTGTCATCTCCGTTGACTATAAGAGCTTTAAAGCTACTTGGACGGAAGTGG TGCACATCAATCGGGAGAGATGGAGGGCCAAGGTACCCAAAGAGGAGAAGGCCAAGAAGGAAGCAGAGGA AAAGGCTCGCCTGGCCGCAGAGGAGCAGCAAAAGGAAATGGAAGCCAAAAGCCAGGCTGAAGAAGGCGCA TCTGGCAAAGCTGAGAAAAAGACGTCTGGAGAAACTAAGAATCAAGTCAATGGAACACGGGCAAACAAAA GTGACAACCCTCGTGGGAAAAACTCCAAAGCCGAGAAGTCATCAGGAGAACAGCAACAGAATGGTGACTT CAAAGATGGTAAAAATAAGACAGACAAGAAGGATCACTCTAACATCGGAAATGATTCAAAGAAAACAGAT GATTCACAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233033 representing NM_001191056 Red=Cloning site Green=Tags(s) MESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV ERMYRRTSNMVGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH IQTRSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIK AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEG SAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEMEAKSQAEEGA SGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTD DSQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191056
ORF Size	1902 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191056.3
RefSeq Size	2979 bp
RefSeq ORF	1905 bp
Locus ID	5137
UniProt ID	Q14123
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Olfactory transduction, Progesterone-mediated oocyte maturation, Purine metabolism
MW	72.7 kDa
Gene Summary	This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016]

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