MELK (NM_001256685) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HPK38
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233011 representing NM_001256685 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGTGGCTTTGCAA AGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAAATCATGGATAAAAACACACT AGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGT CAACTCTACCATGTGCTAGAGACAGCCAACAAAATATTCATGGTTCTTGAGTACTGCCCTGGAGGAGAGC TGTTTGACTATATAATTTCCCAGGATCGCCTGTCAGAAGAGGAGACCCGGGTTGTCTTCCGTCAGATAGT ATCTGCTGTTGCTTATGTGCACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACCCAAGGGTAACAAGGATTACC ATCTACAGACATGCTGTGGGAGTCTGGCTTATGCAGCACCTGAGTTAATACAAGGCAAATCATATCTTGG ATCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATATGTTCTTATGTGTGGATTTCTACCATTTGAT GATGATAATGTAATGGCTTTATACAAGAAGATTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTC CCAGTAGCATTCTGCTTCTTCAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCT ATTGAACCATCCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGCAAAGCAAGAATCCTTTTATT CACCTCGATGATGATTGCGTAACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACAATGGAGGATT TAATTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTTCTAGCCAAGAAGGCTCGGGG AAAACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGACAAGCCAGTGCTACCCCATTCACAGACATC AAGTTTACCAAGTACTGGACAGAATCAAATGGGGTGGAATCTAAATCATTAACTCCAGCCTTATGCAGAA CACCTGCAAATAAATTAAAGAACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTA CTTTATGTTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAATACTCACTACGCCA AATCGTTACACTACACCCTCAAAAGCTAGAAACCAGTGCCTGAAAGAAACTCCAATTAAAATACCAGTAA ATTCAACAGGAACAGACAAGTTAATGACAGGTGTCATTAGCCCTGAGAGGCGGTGCCGCTCAGTGGAATT GGATCTCAACCAAGCACATATGGAGGAGACTCCAAAAAGAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAA AGGGGGTTGGATAAGGTTATCACTGTGCTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCA GAAGACTAAAGCTTCACTATAACGTGACTACAACTAGATTAGTGAATCCAGATCAACTGTTGAATGAAAT AATGTCTATTCTTCCAAAGAAGCATGTTGACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACACAG TCAGATTTTGGGAAAGTGACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAACCCGATGTGGTGG GTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTTTACAAAAGATTAGTGGAAGACATCCTATCTAG CTGCAAGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233011 representing NM_001256685 Red=Cloning site Green=Tags(s) MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHIC QLYHVLETANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLF DEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFD DDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSKNPFI HLDDDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFTDI KFTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNKNQHKREILTTP NRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVISPERRCRSVELDLNQAHMEETPKRKGAKVFGSLE RGLDKVITVLTRSKRKGSARDGPRRLKLHYNVTTTRLVNPDQLLNEIMSILPKKHVDFVQKGYTLKCQTQ SDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256685
ORF Size	1830 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256685.1, NP_001243614.1
RefSeq Size	2363 bp
RefSeq ORF	1833 bp
Locus ID	9833
UniProt ID	Q14680
Protein Families	Druggable Genome, Protein Kinase
MW	70.6 kDa
Gene Summary	Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.[UniProtKB/Swiss-Prot Function]

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