PPP3CC (NM_001243974) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CALNA3; CNA3; PP2Bgamma
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232911 representing NM_001243974 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGGAGGCGCTTCCACCTCTCCACCACCGACCGCGTCATCAAAGCTGTCCCCTTTCCTCCAACCC AACGGCTTACTTTCAAGGAAGTATTTGAGAATGGGAAACCTAAAGTTGATGTTTTAAAAAACCATTTGGT AAAGGAAGGACGACTGGAAGAGGAAGTAGCCTTAAAGATAATCAATGATGGGGCTGCCATCCTGAGGCAA GAGAAGACTATGATAGAAGTAGATGCTCCAATCACAGTATGTGGTGATATTCATGGACAATTCTTTGACC TAATGAAGTTATTTGAAGTTGGAGGATCACCTAGTAACACACGCTACCTCTTTCTGGGTGACTATGTGGA CAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATTTATGGAGTTTAAAGATTAATCATCCCAAAACATTG TTTCTGCTTCGGGGAAATCATGAATGCAGGCATCTTACAGACTATTTCACCTTCAAACAGGAATGTCGAA TCAAATATTCGGAACAGGTGTATGATGCCTGTATGGAGACATTTGACTGTCTTCCTCTTGCTGCCCTCTT AAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCACCTGAAATTACTTCTTTAGATGACATTAGGAAA TTAGACAGGTTTACGGAACCTCCCGCCTTTGGACCTGTGTGTGACCTGCTTTGGTCTGATCCCTCAGAGG ATTATGGCAATGAGAAGACCTTGGAGCACTATACCCACAACACTGTCCGAGGGTGCTCTTATTTCTACAG TTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTTACTATCAATTATCAGAGCCCATGAAGCCCAA GATGCTGGGTATCGAATGTACAGGAAGAGCCAAGCCACAGGCTTTCCATCACTTATTACAATTTTCTCTG CCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCTGTGTTGAAATATGAAAACAATGTCATGAATAT CAGGCAGTTTAACTGTTCTCCACACCCCTACTGGCTTCCAAACTTTATGGATGTTTTCACATGGTCTTTG CCTTTTGTTGGGGAAAAAGTCACAGAGATGCTGGTAAATGTGCTCAACATATGCTCTGATGACGAACTGA TTTCTGATGATGAAGCAGAAGATCACTACATTCCAAGCTATCAGAAAGGAAGCACTACAGTTCGTAAGGA GATCATCAGGAATAAGATCAGAGCCATTGGGAAGATGGCACGGGTCTTTTCAATTCTTCGGCAAGAAAGT GAGAGTGTGCTGACTCTCAAGGGCCTGACTCCCACAGGCACACTCCCTCTGGGCGTCCTCTCAGGAGGCA AGCAGACTATCGAGACAGCCACAGTAGAAGCGGTAGAGGCCCGGGAAGCCATCAGAGGGTTCTCGCTTCA GCACAAGATCCGGAGTTTTGAAGAAGCGCGAGGTCTGGACCGAATTAATGAGCGAATGCCACCCCGAAAG GATAGCATACACGCTGGTGGGCCAATGAAATCTGTAACCTCAGCACACTCACATGCTGCGCACAGGAGCG ACCAAGGGAAGAAAGCCCATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232911 representing NM_001243974 Red=Cloning site Green=Tags(s) MSGRRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGKPKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQ EKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTL FLLRGNHECRHLTDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRK LDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQ DAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSL PFVGEKVTEMLVNVLNICSDDELISDDEAEDHYIPSYQKGSTTVRKEIIRNKIRAIGKMARVFSILRQES ESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRK DSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243974
ORF Size	1563 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243974.1, NP_001230903.1
RefSeq Size	2361 bp
RefSeq ORF	1566 bp
Locus ID	5533
UniProt ID	P48454
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
MW	59.7 kDa
Gene Summary	Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.