GPR161 (NM_001267613) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232766 representing NM_001267613 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGGCGTAGGTGCGTGCCCGGGACTCTGCCGATGCGGGCCGCGCCCCCTGGGGCCAAGAGACTCC ACGTCCCGCTCAGGGCAAAGGGTGTGGGGAGAAGCGGTCACGCCCCTCGGCTGGAATCCGTGAGGACCAG CTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGAAGATCACAGGGAACCGGGCTGTGATGGCACTT GTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTGCCACCCCTGTTTGGTTGGTCATCCGTGGAGTTTG ACGAGTTCAAATGGATGTGTGTGGCTGCTTGGCACCGGGAGCCTGGCTACACGGCCTTCTGGCAGATCTG GTGTGCCCTCTTCCCCTTTCTGGTCATGCTGGTGTGCTATGGCTTCATCTTCCGCGTGGCCAGGGTCAAG GCACGCAAGGTGCACTGTGGCACAGTCGTCATCGTGGAGGAGGATGCTCAGAGGACCGGGAGGAAGAACT CCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAATGCCTTTCAGGGTGTGGTCTACTCGGCCAACCA GTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTGCCTTCATGGTCACCTGGGGCCCCTACATGGTT GTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCCGTCTCCCCGAGCCTGGAGACTTGGGCCACATGGC TGTCCTTTGCCAGCGCTGTCTGCCACCCCCTGATCTATGGACTCTGGAACAAGACAGTTCGCAAAGAACT ACTGGGCATGTGCTTTGGGGACCGGTATTATCGGGAACCATTTGTGCAACGACAGAGGACTTCCAGGCTC TTCAGCATTTCCAACAGGATCACAGACCTGGGCCTGTCCCCACACCTCACTGCGCTCATGGCAGGTGGAC AGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGCTTCAGCTGCTCCCAGGACTCAGGGACAGATAT GATGCTGCTTGAGGACTACACGTCTGATGACAACCCTCCCTCTCACTGCACTTGCCCACCCAAGAGAAGG AGCTCGGTGACATTTGAGGATGAAGTGGAACAAATCAAAGAAGCTGCCAAGAACTCGATTCTTCATGTGA AAGCTGAAGTACACAAGTCCTTGGACAGTTACGCAGCAAGCTTGGCCAAAGCCATTGAGGCCGAAGCCAA AATCAACTTATTTGGGGAGGAGGCTTTGCCAGGGGTCTTGGTTACAGCACGGACTGTCCCGGGGGGCGGC TTCGGGGGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGGCTGCAGTTGCAGAGCATCGAAGAAGGAG ATGTTTTAGCTGCCGAGCAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232766 representing NM_001267613 Red=Cloning site Green=Tags(s) MGGRRCVPGTLPMRAAPPGAKRLHVPLRAKGVGRSGHAPRLESVRTSYYAVLYPMVYPMKITGNRAVMAL VYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWCALFPFLVMLVCYGFIFRVARVK ARKVHCGTVVIVEEDAQRTGRKNSSTSTSSSGSRRNAFQGVVYSANQCKALITILVVLGAFMVTWGPYMV VIASEALWGKSSVSPSLETWATWLSFASAVCHPLIYGLWNKTVRKELLGMCFGDRYYREPFVQRQRTSRL FSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTGFSCSQDSGTDMMLLEDYTSDDNPPSHCTCPPKRR SSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAASLAKAIEAEAKINLFGEEALPGVLVTARTVPGGG FGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001267613
ORF Size	1353 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267613.1, NP_001254542.1
RefSeq Size	7664 bp
RefSeq ORF	1356 bp
Locus ID	23432
UniProt ID	Q8N6U8
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	50.2 kDa
Gene Summary	The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.