BORIS (CTCFL) (NM_001269051) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232666 representing NM_001269051 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCGG AAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGAGTT GGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGAGCTGGTGCTGGCC CCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTGGAGT TGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCC TGGGTTGCTGTGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAA GAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATGGTGGCCA GTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTACTGGACTGATCAAGCTCGAGGAAGAGCAGGA GAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTCTTTTTTGTGGAAACAATGTCAGGAGATGAA AGAAGTGACGAAATTGTTCTCACAGTTTCAAATTCAAATGTGGAAGAACAAGAGGATCAACCTACAGCTG GTCAAGCAGATGCTGAAAAGGCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCAAAAGGAACCTT CCACTGTGATGTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACC AGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATG TTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGA ACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCC AGTGTGGAGGTAAAGCCATTCTTGGACTTGAAGCTTCATGGCATCTTAGTAGAGGCTGCTGTACAAGTTA CTCCAAGTGTAACTAACAGTAGAATCTGTTACAAACAGGCTTTTTATTATTCATATAAAATTTATGCAGG AAATAATATGCATTCTCTTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232666 representing NM_001269051 Red=Cloning site Green=Tags(s) MAATEISVLSEQFTKIKELELMPEKGLKEEEKDGVCREKDHRSPSELEAERTSGAFQDSVLEEEVELVLA PSEESEKYILTLQTVHFTSEAVELQDMSLLSIQQQEGVQVVVQQPGPGLLWLEEGPRQSLQQCVAISIQQ ELYSPQEMEVLQFHALEENVMVASEDSKLAVSLAETTGLIKLEEEQEKNQLLAERTKEQLFFVETMSGDE RSDEIVLTVSNSNVEEQEDQPTAGQADAEKAKSTKNQRKTKGAKGTFHCDVCMFTSSRMSSFNRHMKTHT SEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHRRYKHTHEKPFKCSMCKYA SVEVKPFLDLKLHGILVEAAVQVTPSVTNSRICYKQAFYYSYKIYAGNNMHSLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001269051
ORF Size	1212 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001269051.1, NP_001255980.1
RefSeq Size	1513 bp
RefSeq ORF	1215 bp
Locus ID	140690
UniProt ID	Q8NI51
Protein Families	Transcription Factors
MW	46.3 kDa
Gene Summary	CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

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