SEPTIN5 (NM_001009939) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDCREL; CDCREL-1; CDCREL1; H5; HCDCREL-1; PNUTL1; SEPT5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232540 representing NM_001009939 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGCTGGCAGCGCCCCAGGACCGCCTGGTGGAGCAGCTGCTGTCGCCGCGGACCCAGGCCCAGA GGCGGCTCAAGGACATTGACAAGCAGTACGTGGGCTTCGCCACACTGCCCAACCAGGTGCACCGCAAGTC GGTGAAGAAAGGCTTTGACTTCACACTCATGGTGGCTGGTGAGTCAGGCCTGGGGAAGTCCACACTGGTC CACAGCCTCTTCCTGACAGACTTGTACAAGGACCGGAAGCTGCTCAGTGCTGAGGAGCGCATCAGCCAGA CGGTAGAGATTCTAAAACACACGGTGGACATTGAGGAGAAGGGAGTCAAGCTGAAGCTCACCATCGTGGA CACGCCGGGATTCGGGGACGCTGTCAACAACACCGAGTGCTGGAAGCCCATCACCGACTATGTGGACCAG CAGTTTGAGCAGTACTTCCGTGATGAGAGCGGCCTCAACCGAAAGAACATCCAAGACAACCGAGTGCACT GCTGCCTATACTTCATCTCCCCCTTCGGGCATGGGCTGCGGCCAGTGGATGTGGGTTTCATGAAGGCATT GCATGAGAAGGTCAACATCGTGCCTCTCATCGCCAAAGCTGACTGTCTTGTCCCCAGTGAGATCCGGAAG CTGAAGGAGCGGATCCGGGAGGAGATTGACAAGTTTGGGATCCATGTATACCAGTTCCCTGAGTGTGACT CGGACGAGGATGAGGACTTCAAGCAGCAGGACCGGGAACTGAAGGAGAGCGCGCCCTTCGCCGTTATAGG CAGCAACACGGTGGTGGAGGCCAAGGGGCAGCGGGTCCGGGGCCGACTGTACCCCTGGGGGATCGTGGAG GGCGCATTGCGACTTCGTGAAGCTGCGCAACATGCTCATCCGCACGCATATGCACGACCTCAAGGACGTG ACGTGCGACGTGCACTACGAGAACTACCGCGCGCACTGCATCCAGCAGATGACCAGCAAACTGACCCAGG ACAGCCGCATGGAGAGCCCCATCCCGATCCTGCCGCTGCCCACCCCGGACGCCGAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232540 representing NM_001009939 Red=Cloning site Green=Tags(s) MDSLAAPQDRLVEQLLSPRTQAQRRLKDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLV HSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQ QFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRK LKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVE GALRLREAAQHAHPHAYARPQGRDVRRALRELPRALHPADDQQTDPGQPHGEPHPDPAAAHPGRRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001009939
ORF Size	1038 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001009939.3
RefSeq Size	2284 bp
RefSeq ORF	1041 bp
Locus ID	5413
UniProt ID	Q99719
Protein Families	Druggable Genome
Protein Pathways	Parkinson's disease
MW	39.8 kDa
Gene Summary	This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.