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DCTN1 (NM_001190836) Human Tagged ORF Clone
CAT＃: RC231476
- TrueORF®
DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 5
ORF Plasmid: tGFP
"NM_001190836" in other vectors (3)
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CNY 12,350.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DAP-150; DP-150; P135 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231476 representing NM_001190836
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCGGAGGCAAGCGCCCGGCCTCTGCGGGTGGGCTCCCGTGTAGAGGTGATTGGAAAAGGCCACC GAGGCACTGTGGCCTATGTTGGAGCCACACTGTTTGCCACTGGCAAATGGGTAGGCGTGATTCTGGATGA AGCAAAGGGCAAAAATGATGGAACTGTTCAAGGCAGGAAGTACTTCACTTGTGATGAAGGGCATGGCATC TTTGTGCGCCAGTCCCAGATCCAGGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACACCTGATT CTTCTGCTTCAAAAGTCCTCAAAAGAGAGGGAACTGATACAACTGCAAAGACTAGCAAACTGCCCACGCG CCCAGCCAGTACTGGGGTGGCTGGGGCCAGTAGCTCCCTGGGCCCCTCTGGCTCAGCGTCAGCAGGTGAG CTGAGCAGCAGTGAGCCCAGCACCCCGGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACGCCGGTCC TCACCTCTCCTGGAGCAGTCCCCCCGCTTCCTTCCCCATCCAAGGAGGAGGAGGGACTAAGGGCTCAGGT GCGGGACCTGGAGGAGAAACTAGAGACCCTGAGACTGAAACGGGCAGAAGACAAAGCAAAGCTAAAAGAG CTGGAGAAACACAAAATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCCG ACCTGCAGCGGCGCCTCAAGGAGGCGAGAAAGGAAGCCAAGGAGGCGCTGGAGGCAAAGGAACGCTATAT GGAGGAGATGGCTGATACTGCTGATGCCATTGAGATGGCCACTTTGGACAAGGAGATGGCTGAAGAGCGG GCTGAGTCCCTGCAGCAGGAGGTGGAGGCACTGAAGGAGCGGGTGGACGAGCTCACTACTGACTTAGAGA TCCTCAAGGCTGAGATTGAAGAGAAGGGCTCAGATGGCGCTGCATCCAGTTATCAGCTCAAGCAGCTTGA GGAGCAGAATGCCCGCCTGAAGGATGCCCTGGTGAGGATGCGGGATCTTTCTTCCTCAGAGAAGCAGGAG CATGTGAAGCTCCAGAAGCTCATGGAAAAGAAGAACCAAGAGCTGGAAGTTGTGAGGCAACAGCGGGAGC GTCTGCAGGAGGAGCTAAGCCAGGCAGAGAGCACCATTGATGAGCTCAAGGAGCAGGTGGATGCTGCTCT GGGTGCTGAGGAGATGGTGGAGATGCTGACAGATCGGAACCTGAATCTGGAAGAGAAAGTGCGCGAGTTG AGGGAGACTGTGGGAGACTTGGAAGCGATGAATGAGATGAACGATGAGCTGCAGGAGAATGCACGTGAGA CAGAACTGGAGCTGCGGGAGCAGCTGGACATGGCAGGCGCGCGGGTTCGTGAGGCCCAGAAGCGTGTGGA GGCAGCCCAGGAGACGGTTGCAGACTACCAGCAGACCATCAAGAAGTACCGCCAGCTGACCGCCCATCTA CAGGATGTGAATCGGGAACTGACAAACCAGCAGGAAGCATCTGTGGAGAGGCAACAGCAGCCACCTCCAG AGACCTTTGACTTCAAAATCAAGTTTGCTGAGACTAAGGCCCATGCCAAGGCAATTGAGATGGAATTGAG GCAGATGGAGGTGGCCCAGGCCAATCGACACATGTCCCTGCTGACAGCCTTCATGCCTGACAGCTTCCTT CGGCCAGGTGGGGACCATGACTGCGTTCTGGTGCTGTTGCTCATGCCTCGTCTCATTTGCAAGGCAGAGC TGATCCGGAAGCAGGCCCAGGAGAAGTTTGAACTAAGTGAGAACTGTTCAGAGCGGCCTGGGCTGCGAGG AGCTGCTGGGGAGCAACTCAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGCCTGCTGCAGGCCACGCTA CACCGCTATGAGCATGCCCTCTCTCAGTGCAGTGTGGATGTGTATAAGAAAGTGGGCAGCCTGTACCCTG AGATGAGTGCCCATGAGCGCTCCTTGGATTTCCTCATTGAACTGCTGCACAAGGATCAGCTGGATGAGAC TGTCAATGTGGAGCCTCTCACCAAGGCCATCAAGTACTATCAGCATCTGTACAGCATCCACCTTGCCGAA CAGCCTGAGGACTGTACTATGCAGCTGGCTGACCACATTAAGTTCACGCAGAGTGCTCTGGACTGCATGA GTGTGGAGGTAGGACGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCTACAGATATTGCCCTCCTGCT CCGGGATCTGGAAACTTCATGCAGTGACATCCGCCAGTTCTGCAAGAAGATCCGAAGGCGAATGCCAGGG ACAGATGCTCCTGGGATCCCAGCTGCACTGGCCTTTGGACCACAGGTATCTGACACGCTCCTAGACTGCA GGAAACACTTGACGTGGGTCGTGGCTGTGCTGCAGGAGGTGGCAGCTGCTGCTGCCCAGCTCATTGCCCC ACTGGCAGAGAATGAGGGGCTACTTGTGGCTGCTCTGGAGGAACTGGCTTTCAAAGCAAGCGAGCAGATC TATGGGACCCCCTCCAGCAGCCCCTATGAGTGTCTGCGCCAGTCATGCAACATCCTCATCAGTACCATGA ACAAGCTGGCCACAGCCATGCAGGAGGGGGAGTATGATGCAGAGCGGCCCCCCAGCAAGCCTCCACCGGT TGAACTGCGGGCTGCTGCCCTTCGTGCAGAGATCACAGATGCTGAAGGCCTGGGTTTGAAGCTCGAAGAT CGAGAGACAGTTATTAAGGAGTTGAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTAAGTGAGGCCAATG TGCGGCTGAGCCTCCTGGAGAAGAAGTTGGACAGTGCTGCCAAGGATGCAGATGAGCGCATCGAGAAAGT CCAGACTCGGCTGGAGGAGACCCAGGCACTGCTGCGAAAGAAGGAGAAAGAGTTTGAGGAGACAATGGAT GCACTCCAGGCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAACTAAAGCAGCGTCTGAACAGCCAGT CCAAACGCACGATTGAGGGACTCCGGGGCCCTCCTCCTTCAGGCATTGCTACTCTGGTCTCTGGCATTGC TGGTGGAGCCATCCCTGGGCAGGCTCCAGGGTCTGTGCCAGGCCCAGGGCTGGTGAAGGACTCACCACTG CTGCTTCAGCAGATCTCTGCCATGAGGCTGCACATCTCCCAGCTCCAGCATGAGAACAGCATCCTCAAGG GAGCCCAGATGAAGGCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGCAAAGCTATCCCATGAGGGCCC TGGCAGTGAGTTACCAGCTGGAGCGCTGTATCGTAAGACCAGCCAGCTGCTGGAGACATTGAATCAATTG AGCACACACACGCACGTAGTAGACATCACTCGCACCAGCCCTGCTGCCAAGAGCCCGTCGGCCCAACTTA TGGAGCAAGTGGCTCAGCTTAAGTCCCTGAGTGACACCGTCGAGAAGCTCAAGGATGAGGTCCTCAAGGA GACAGTATCTCAGCGCCCTGGAGCCACAGTACCCACTGACTTTGCCACCTTCCCTTCATCAGCCTTCCTC AGGGCCAAGGAGGAGCAGCAGGATGACACAGTCTACATGGGCAAAGTGACCTTCTCATGTGCGGCTGGTT TTGGACAGCGACACCGGCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCTCATCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231476 representing NM_001190836
Red=Cloning site Green=Tags(s) MSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGI FVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLPTRPASTGVAGASSSLGPSGSASAGE LSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKE LEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEER AESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQE HVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVREL RETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHL QDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFL RPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATL HRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAE QPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPG TDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQI YGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLED RETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMD ALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGGAIPGQAPGSVPGPGLVKDSPL LLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQL STHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFL RAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190836 |
| ORF Size | 3708 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190836.2 |
| RefSeq ORF | 3711 bp |
| Locus ID | 1639 |
| UniProt ID | Q14203 |
| Protein Families | Druggable Genome |
| Protein Pathways | Huntington's disease |
| MW | 137.3 kDa |
| Gene Summary | This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231476L3 | Lenti-ORF clone of DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 5 |
CNY 14,250.00 |
|
| RC231476L4 | Lenti-ORF clone of DCTN1 (mGFP-tagged)-Human dynactin 1 (DCTN1), transcript variant 5 |
CNY 14,250.00 |
|
| RG231476 | DCTN1 (tGFP-tagged) - Human dynactin 1 (DCTN1), transcript variant 5 |
CNY 13,590.00 |
