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Selenophosphate synthetase 1 (SEPHS1) (NM_001195604) Human Tagged ORF Clone
CAT＃: RC231162
SEPHS1 (Myc-DDK-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3
ORF Plasmid: tGFP
"NM_001195604" in other vectors (3)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SELD; SPS; SPS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231162 representing NM_001195604
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACGCGGGAGTCCTTTAACCCGGAAAGTTACGAATTGGACAAAAGCTTCCGGCTAACCAGATTCA CTGAACTGAAGGGCACAGGCTGCAAAGTGCCCCAAGATGTCCTGCAAAAATTGCTGGAATCTTTACAGGA GAACCACTTCCAAGAAGATGAGCAGTTTCTGGGAGCCGTTATGCCAAGGCTTGGCATTGGAATGGATACT TGTGTCATTCCTTTGAGGCACGGTGGGCTTTCCTTGGTTCAAACCACAGATTACATTTACCCGATCGTAG ACGACCCTTACATGATGGGCAGGATAGCGTGTGCCAATGTCCTCAGTGACCTCTATGCAATGGGGGTCAC GGAATGTGACAATATGCTGATGCTCCTTGGAGTCAGTAATAAAATGACCGACAGGGAAAGGGATAAAGTG ATGCCTCTGATTATCCAAGGTTTTAAAGACGCAGCTGAGGAAGCAGGAACATCTGTAACAGGCGGCCAAA CAGTACTAAACCCCTGGATTGTCCTGGGAGGAGTGGCTACCACTGTCTGCCAACCCAATGAATTTATCAT GCCAGACAATGCAGTGCCAGGGGACGTGCTGGTGCTGACAAAACCCCTGGGGACACAGGTGGCAGTGGCT GTGCACCAGTGGCTGGATATCCCTGAGAAATGGAATAAGATTAAACTAGTGGTCACCCAAGAAGATGTAG AGCTGGCCTACCAGGAGGCGATGATGAACATGGCGAGGCTCAACAGGACAGGCGGCCTTCTGATCTGTTT ACCACGTGAGCAAGCAGCTCGGTTCTGTGCAGAGATAAAGTCCCCCAAATATGGTGAAGGCCACCAAGCA TGGATTATTGGGATTGTAGAGAAGGGCAACCGCACAGCCAGAATCATAGACAAACCCCGGATCATCGAGG TCGCACCACAAGTGGCCACTCAAAATGTGAATCCCACACCCGGGGCCACCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231162 representing NM_001195604
Red=Cloning site Green=Tags(s) MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDT CVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKV MPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVA VHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTGGLLICLPREQAARFCAEIKSPKYGEGHQA WIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195604 |
| ORF Size | 963 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001195604.2 |
| RefSeq ORF | 966 bp |
| Locus ID | 22929 |
| UniProt ID | P49903 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Metabolic pathways, Selenoamino acid metabolism |
| MW | 35.9 kDa |
| Gene Summary | This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231162L3 | Lenti ORF clone of Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC231162L4 | Lenti ORF clone of Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG231162 | SEPHS1 (tGFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3 |
CNY 4,370.00 |
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