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PC1/3 (PCSK1) (NM_001177875) Human Tagged ORF Clone
CAT＃: RC230447
- TrueORF®
PCSK1 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 2
ORF Plasmid: tGFP
"NM_001177875" in other vectors (4)
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CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BMIQ12; NEC1; PC1; PC3; SPC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_001177875 ORF sequence, RC230447 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAAGGGATCTATTTCTTTCTTGTTCTTCAGCCAAATTGGTTCACTTGAAAATCACTACTTATTC AAACATAAAAACCACCCCAGAAGGTCTCGAAGGAGTGCCTTTCATATCACTAAGAGATTATCTGATGAT GATCGTGTGATATGGGCTGAACAACAGTATGAAAAAGAAAGAAGTAAACGTTCAGCTCTAAGGGACTCA GCACTAAATCTCTTCAATGATCCCATGTGGAATCAGCAATGGTACTTGCAAGATACCAGGATGACGGCA GCCCTGCCCAAGCTGGACCTTCATGTGATACCTGTTTGGCAAAAAGGCATTACGGGCAAAGGAGTTGTT ATCACCGTACTGGATGATGGTTTGGAGTGGAATCACACGGACATTTATGCCAACTATGATCCAGAGGCT AGCTATGATTTTAATGATAATGACCATGATCCATTTCCCCGATATGATCCCACAAACGAGAACAAACAC GGGACCAGATGTGCAGGAGAAATTGCCATGCAAGCAAATAATCACAAATGCGGGGTTGGAGTTGCATAC AATTCCAAAGTTGGAGGCATAAGAATGCTGGATGGCATTGTGACGGATGCTATTGAGGCCAGTTCAATT GGATTCAATCCTGGACACGTGGATATTTACAGTGCAAGCTGGGGCCCTAATGATGATGGGAAAACTGTG GAGGGGCCTGGCCGGCTAGCCCAGAAGGCTTTTGAATATGGTGTCAAACAGGGGAGACAGGGGAAGGGG TCCATCTTCGTCTGGGCTTCGGGAAACGGGGGGCGTCAGGGAGATAATTGTGACTGTGATGGCTACACA GACAGCATCTACACCATCTCCATCAGCAGTGCCTCCCAGCAAGGCCTATCCCCCTGGTACGCTGAGAAG TGCTCCTCCACACTGGCCACCTCTTACAGCAGCGGAGATTACACCGACCAGAGAATCACGAGCGCTGAC CTGCACAATGACTGCACGGAGACGCACACAGGCACCTCGGCCTCTGCACCTCTGGCTGCTGGCATCTTC GCTCTGGCCCTGGAAGCAAACCCAAATCTCACCTGGCGAGATATGCAGCACCTGGTTGTCTGGACCTCT GAGTATGACCCGCTGGCCAATAACCCTGGATGGAAAAAGAATGGAGCAGGCTTGATGGTGAATAGTCGA TTTGGATTTGGCTTGCTAAATGCCAAAGCTCTGGTGGATTTAGCTGACCCCAGGACCTGGAGGAGCGTG CCTGAGAAGAAAGAGTGTGTTGTAAAGGACAATGACTTTGAGCCCAGAGCCCTGAAAGCTAATGGAGAA GTTATCATTGAAATTCCAACAAGAGCTTGTGAAGGACAAGAAAATGCTATCAAGTCCCTGGAGCATGTA CAATTTGAAGCAACAATTGAATATTCCCGAAGAGGAGACCTTCATGTCACACTTACTTCTGCTGCTGGA ACTAGCACTGTGCTCTTGGCTGAAAGAGAACGGGATACATCTCCTAATGGCTTTAAGAATTGGGACTTC ATGTCTGTTCACACATGGGGAGAGAACCCTATAGGTACTTGGACTTTGAGAATTACAGACATGTCTGGA AGAATTCAAAATGAAGGAAGAATTGTGAACTGGAAGCTGATTTTGCACGGGACCTCTTCTCAGCCAGAG CATATGAAGCAGCCTCGTGTGTACACGTCCTACAACACTGTTCAGAATGACAGAAGAGGGGTGGAGAAG ATGGTGGATCCAGGGGAGGAGCAGCCCACACAAGAGAACCCTAAGGAGAACACCCTGGTGTCCAAAAGC CCCAGCAGCAGCAGCGTAGGGGGCCGGAGGGATGAGTTGGAGGAGGGAGCCCCTTCCCAGGCCATGCTG CGACTCCTGCAAAGTGCTTTCAGTAAAAACTCACCGCCAAAGCAATCACCAAAGAAGTCCCCAAGTGCA AAGCTCAACATCCCTTATGAAAACTTCTACGAAGCCCTGGAAAAGCTGAACAAACCTTCCCAGCTTAAA GACTCTGAAGACAGTCTGTATAATGACTATGTTGATGTTTTTTATAACACTAAACCTTACAAGCACAGA GACGACCGGCTGCTTCAAGCTCTGGTGGACATTCTGAATGAGGAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC230447
Blue=ORF Red=Cloning site Green=Tag(s) MGKGSISFLFFSQIGSLENHYLFKHKNHPRRSRRSAFHITKRLSDDDRVIWAEQQYEKERSKRSALRDS ALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEA SYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSI GFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYT DSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIF ALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSV PEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAG TSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIVNWKLILHGTSSQPE HMKQPRVYTSYNTVQNDRRGVEKMVDPGEEQPTQENPKENTLVSKSPSSSSVGGRRDELEEGAPSQAML RLLQSAFSKNSPPKQSPKKSPSAKLNIPYENFYEALEKLNKPSQLKDSEDSLYNDYVDVFYNTKPYKHR DDRLLQALVDILNEEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177875 |
| ORF Size | 2118 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177875.2 |
| RefSeq Size | 4828 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 5122 |
| UniProt ID | P29120 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 79 kDa |
| Gene Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230447L3 | Lenti-ORF clone of PCSK1 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 2 |
CNY 9,310.00 |
|
| RC230447L4 | Lenti-ORF clone of PCSK1 (mGFP-tagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 2 |
CNY 9,310.00 |
|
| RG230447 | PCSK1 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 2 |
CNY 8,170.00 |
|
| SC329085 | PCSK1 (untagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1) transcript variant 2 |
CNY 5,176.00 |
