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DNA Polymerase lambda (POLL) (NM_001174085) Human Tagged ORF Clone
CAT＃: RC230157
POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 3
ORF Plasmid: tGFP
"NM_001174085" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BETAN; POLKAPPA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230157 representing NM_001174085
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTGATGCATCATCAAAAG TACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAGAGTGGCTGAGCTCCCTTCGGGCCCA TGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAGAACTCTTTGAGAAGCAGATTGTTCAGCATGGCGGC CAGCTATGCCCTGCCCAGGGCCCAGGTGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAG CCCTCCGCCTTCTCAGACTACCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTT GTGCCTTCAGGAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACTTGGAC CATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATGAGGCCCTGCTTCAGA CAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTCCCCAAAAGGCAAAAGAGGCACCAAA CACCCAAGCCCAGCCCATCTCTGATGATGAAGCCAGTGATGGGGAAGAAACCCAGGAGGCCTGCAGTATC CCTGGGATTGGGAAGCGGATGGCTGAGAAAATCATAGAGATCCTGGAGAGCGGGCATTTGCGGAAGCTGG ACCATATCAGTGAGAGCGTGCCTGTCTTGGAGCTCTTCTCCAACATCTGGGGAGCTGGGACCAAGACTGC CCAGATGTGGTACCAACAGGGCTTCCGAAGTCTGGAAGACATCCGCAGCCAGGCCTCCCTGACAACCCAG CAGGCCATCGGCCTGAAGCATTACAGTGACTTCCTGGAACGTATGCCCAGGGAGGAGGCTACAGAGATTG AGCAGACAGTCCAGAAAGCAGCCCAGGCCTTTAACTCTGGGCTGCTGTGTGTGGCATGTGGTTCATACCG ACGGGGAAAGGCGACCTGTGGTGATGTCGACGTGCTCATCACTCACCCAGATGGCCGGTCCCACCGGGGT ATCTTCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATGACTTGGTGAGCCAAGAGG AGAATGGTCAGCAACAGAAGTACTTGGGGGTGTGCCGGCTCCCAGGGCCAGGGCGGCGGCACCGGCGCCT GGACATCATCGTGGTGCCCTATAGCGAGTTTGCCTGTGCCCTGCTCTACTTCACCGGCTCTGCACACTTC AACCGCTCCATGCGAGCCCTGGCCAAAACCAAGGGCATGAGTCTGTCAGAACATGCCCTCAGCACTGCTG TGGTCCGGAACACCCATGGCTGCAAGGTGGGGCCTGGCCGAGTGCTGCCCACTCCCACTGAGAAGGATGT CTTCAGGCTCTTAGGCCTCCCCTACCGAGAACCTGCTGAGCGGGACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230157 representing NM_001174085
Red=Cloning site Green=Tags(s) MDPRGILKAFPKRQKIHADASSKVLAKIPRREEGEEAEEWLSSLRAHVVRTGIGRARAELFEKQIVQHGG QLCPAQGPGVTHIVVDEGMDYERALRLLRLPQLPPGAQLVKSAWLSLCLQERRLVDVAGFSIFIPSRYLD HPQPSKAEQDASIPPGTHEALLQTALSPPPPPTRPVSPPQKAKEAPNTQAQPISDDEASDGEETQEACSI PGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQGFRSLEDIRSQASLTTQ QAIGLKHYSDFLERMPREEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRG IFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFTGSAHF NRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTEKDVFRLLGLPYREPAERDW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001174085 |
| ORF Size | 1449 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174085.1, NP_001167556.1 |
| RefSeq ORF | 1452 bp |
| Locus ID | 27343 |
| UniProt ID | Q9UGP5 |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, Non-homologous end-joining |
| MW | 54 kDa |
| Gene Summary | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Polλ promotes microhomology-mediated end-joining
,null,
Nature structural & molecular biology
,PubMed ID 36536104
[DNA Polymerase lambda]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230157L3 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC230157L4 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG230157 | POLL (tGFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 3 |
CNY 4,370.00 |
|
| SC328795 | POLL (untagged)-Human polymerase (DNA directed) lambda (POLL) transcript variant 3 |
CNY 7,790.00 |
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