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Asparagine synthetase (ASNS) (NM_001178076) Human Tagged ORF Clone
CAT＃: RC230144
- TrueORF®
ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 5
ORF Plasmid: tGFP
"NM_001178076" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASNSD; TS11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230144 representing NM_001178076
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGTGAGATAATCCTTCATCTTTATGACA AAGGAGGAATTGAGCAAACAATTTGTATGTTGGATGGTGTGTTTGCATTTGTTTTACTGGATACTGCCAA TAAGAAAGTGTTCCTGGGTAGAGATACATATGGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGA TTTTTGGCTGTATGTTCAGAAGCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAG TGGAGCCTTTTCTTCCTGGACACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATCCGTGGA AATGGTTAAATATCATCACTGTCGGGATGTACCCCTGCACGCCCTCTATGACAATGTGGAGAAACTCTTT CCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCTTTTTAATAATGCTGTAAAGAAACGTT TGATGACAGACAGAAGGATTGGCTGCCTTTTATCAGGGGGCTTGGACTCCAGCTTGGTTGCTGCCACTCT GTTGAAGCAGCTGAAAGAAGCCCAAGTACAGTATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGC CCCGATTTACTGGCTGCTAGAAAGGTGGCAGATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACT CTGAGGAAGGCATTCAGGCTCTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAACAGTTCG TGCTTCAGTAGGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAGCGTGGTGATCTTCTCT GGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCACAAGGCTCCTTCTCCTGAAAAAGCCG AGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTATTTGTTTGATGTTCTCCGCGCAGATCGAACTACTGC TGCCCATGGTCTTGAACTGAGAGTCCCATTTCTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCA CCAGAAATGAGAATTCCAAAGAATGGGATAGAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATC TGATACCCAAAGAGATTCTCTGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAAGAATTC CTGGTTTAAGATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGATGGCAAATGCAGCCCAG AAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTACCGTCAAGTCTTTGAACGCCATTACC CAGGCCGGGCTGACTGGCTGAGCCATTACTGGATGCCCAAGTGGATCAATGCCACTGACCCTTCTGCCCG CACGCTGACCCACTACAAGTCAGCTGTCAAAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230144 representing NM_001178076
Red=Cloning site Green=Tags(s) MQQHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDG FLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLF PGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDS PDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFS GEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLP PEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQ KFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001178076 |
| ORF Size | 1434 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001178076.2 |
| RefSeq ORF | 1437 bp |
| Locus ID | 440 |
| UniProt ID | P08243 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism |
| MW | 55.3 kDa |
| Gene Summary | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230144L3 | Lenti-ORF clone of ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 5 |
CNY 6,180.00 |
|
| RC230144L4 | Lenti-ORF clone of ASNS (mGFP-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 5 |
CNY 6,180.00 |
|
| RG230144 | ASNS (tGFP-tagged) - Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 5 |
CNY 4,750.00 |
|
| SC328782 | ASNS (untagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS) transcript variant 5 |
CNY 7,700.00 |
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