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p53R2 (RRM2B) (NM_001172478) Human Tagged Lenti ORF Clone
CAT＃: RC229848L4
- LentiORF®
Lenti ORF clone of Human ribonucleotide reductase M2 B (TP53 inducible) (RRM2B), transcript variant 3, mGFP tagged
"NM_001172478" in other vectors (4)
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CNY 5,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | MTDPS8A; MTDPS8B; P53R2 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC229848).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172478 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172478.1 |
| RefSeq ORF | 900 bp |
| Locus ID | 50484 |
| UniProt ID | Q7LG56 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Glutathione metabolism, Metabolic pathways, p53 signaling pathway, Purine metabolism, Pyrimidine metabolism |
| MW | 35 kDa |
| Gene Summary | This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229848 | RRM2B (Myc-DDK-tagged)-Human ribonucleotide reductase M2 B (TP53 inducible) (RRM2B), transcript variant 3 |
CNY 2,400.00 |
|
| RC229848L3 | Lenti ORF clone of Human ribonucleotide reductase M2 B (TP53 inducible) (RRM2B), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG229848 | RRM2B (tGFP-tagged) - Human ribonucleotide reductase M2 B (TP53 inducible) (RRM2B), transcript variant 3 |
CNY 4,370.00 |
|
| SC328486 | RRM2B (untagged)-Human ribonucleotide reductase M2 B (TP53 inducible) (RRM2B) transcript variant 3 |
CNY 6,270.00 |
