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AP1B1 (NM_001166019) Human Tagged ORF Clone
CAT＃: RC229039
- TrueORF®
AP1B1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 3
ORF Plasmid: tGFP
"NM_001166019" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADTB1; AP105A; BAM22; CLAPB2; KIDAR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229039 representing NM_001166019
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACTCAAAATATTTCACCACGACCAAGAAAGGGGAGATCTTCGAGCTGAAGGCAGAGCTCAACA GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCGATGACCGTGGGCAAAGATGT CAGTGCCCTCTTCCCCGATGTGGTCAACTGCATGCAGACGGACAACCTGGAGCTGAAGAAGCTGGTATAC CTCTACTTGATGAATTACGCCAAGAGTCAGCCTGACATGGCCATTATGGCCGTCAACACCTTTGTGAAGG ACTGTGAGGACCCCAACCCCCTCATCCGAGCCCTGGCAGTGCGGACCATGGGCTGCATCCGCGTTGACAA GATCACAGAGTACCTGTGCGAGCCACTCCGGAAGTGCCTGAAGGACGAGGATCCATATGTGCGCAAGACA GCAGCTGTGTGCGTGGCCAAGCTCCACGACATCAACGCCCAGCTGGTGGAGGACCAGGGCTTCCTGGACA CCCTTAAAGACCTCATCTCCGACTCTAACCCCATGGTGGTGGCCAATGCAGTGGCAGCGCTCTCAGAAAT TGCCGAGTCTCACCCCAGCAGCAACCTGCTCGATCTGAACCCACAGTCCATCAACAAGCTGCTGACAGCC CTCAATGAGTGCACCGAGTGGGGCCAGATCTTCATCCTGGACTGCCTCGCCAACTATATGCCCAAGGACG ACCGCGAGGCCCAGAGCATCTGTGAGCGGGTCACCCCCAGGCTCTCCCATGCCAACTCCGCTGTGGTGCT CTCTGCTGTGAAGGTGCTGATGAAGTTCATGGAGATGTTGTCTAAGGACTTGGACTACTACGGCACACTG CTCAAGAAGCTGGCCCCACCCCTGGTCACACTGCTGTCAGCCGAGCCAGAGCTGCAGTATGTGGCCCTGC GCAACATCAATCTCATCGTGCAGAAAAGGCCTGAGATCCTGAAGCATGAGATGAAGGTGTTCTTCGTGAA GTACAACGACCCTATCTACGTGAAGCTGGAGAAGCTGGACATCATGATCCGCCTGGCCTCTCAGGCCAAC ATCGCCCAGGTGTTGGCAGAGCTGAAAGAGTACGCAACAGAAGTGGATGTGGACTTTGTACGGAAGGCTG TGCGTGCTATTGGCCGCTGCGCCATCAAGGTGGAGCAATCTGCGGAGCGCTGTGTGAGCACGCTGCTCGA CCTCATCCAGACCAAGGTCAACTATGTGGTCCAGGAGGCCATCGTGGTCATCAAGGACATCTTCCGCAAG TACCCCAACAAGTATGAGAGTGTGATTGCCACACTGTGTGAGAATCTGGACTCCCTGGATGAGCCTGAGG CCCGGGCTGCCATGATCTGGATTGTGGGCGAGTACGCGGAACGGATCGACAACGCAGATGAGCTGCTGGA GAGCTTCCTCGAGGGCTTCCATGACGAGAGCACACAGGTCCAGCTGCAGCTGCTGACAGCCATTGTGAAA CTCTTTCTAAAGAAGCCAACAGAGACCCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT CAGATAACCCAGACCTGCGGGACCGTGGCTACATCTACTGGCGCCTGCTGTCCACGGACCCGGTGGCAGC CAAGGAGGTGGTGTTGGCTGAGAAGCCACTCATCTCTGAAGAGACGGACCTCATCGAGCCCACACTGTTA GACGAGCTTATCTGCTACATCGGCACGCTGGCTTCCGTCTACCATAAGCCTCCCAGTGCCTTTGTGGAGG GGGGCCGGGGCGTCGTGCACAAGAGCCTGCCACCTCGCACGGCCTCGAGTGAGAGCGCAGAGAGCCCTGA GACAGCCCCTACTGGAGCACCTCCTGGGGAGCAGCCAGATGTCATCCCCGCCCAGGGCGACCTGCTGGGT GACCTCCTCAACCTGGACCTCGGCCCCCCAGTGAGCGGCCCACCCCTGGCCACCTCCTCGGTGCAGATGG GAGCTGTGGACCTTCTTGGCGGTGGCCTTGACAGCCTGATTGGGGGCACCAACTTCGTGGCACCTCCAAC AGCAGCAGTACCAGCCAATCTTGGAGCACCCATCGGCAGTGGCCTGAGTGACCTCTTTGACCTGACCAGT GGCGTGGGCACCCTGTCAGGATCATATGTGGCCCCCAAAGCAGTGGGCTCCATCTCCATGGACCTGCAGC TGACCAACAAGGCCTTGCAGGTCATGACCGACTTTGCCATCCAGTTCAACCGCAACAGCTTTGGCCTGGC CCCCGCCGCCCCCCTCCAGGTCCACGCACCACTCAGCCCCAACCAGACAGTGGAGATCTCCCTGCCTCTC AGCACGGTGGGCTCGGTCATGAAGATGGAGCCTCTGAACAACCTCCAGGTGGCCGTGAAGAACAACATCG ATGTCTTCTACTTCAGCACCTTGTACCCACTGCACATCCTCTTTGTGGAGGACGGGAAGATGGACCGGCA GATGTTCCTGGCCACATGGAAGGATATTCCCAATGAGAATGAGGCCCAGTTCCAGATCAGAGACTGCCCC CTCAATGCAGAGGCTGCGAGCAGCAAGCTGCAGAGCAGCAACATCTTCACTGTCGCCAAGAGGAACGTGG AGGGCCAGGACATGCTCTACCAGTCCCTGAAGCTGACCAACGGCATCTGGGTGCTGGCGGAGCTGCGGAT CCAGCCGGGCAACCCCAGCTGCACGCTGTCCCTGAAGTGTCGAGCACCAGAGGTGTCCCAGCACGTGTAC CAGGCCTACGAGACCATCCTCAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229039 representing NM_001166019
Red=Cloning site Green=Tags(s) MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVY LYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT AAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTA LNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTL LKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQAN IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRK YPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVK LFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLL DELICYIGTLASVYHKPPSAFVEGGRGVVHKSLPPRTASSESAESPETAPTGAPPGEQPDVIPAQGDLLG DLLNLDLGPPVSGPPLATSSVQMGAVDLLGGGLDSLIGGTNFVAPPTAAVPANLGAPIGSGLSDLFDLTS GVGTLSGSYVAPKAVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPL STVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCP LNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSCTLSLKCRAPEVSQHVY QAYETILKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166019 |
| ORF Size | 2757 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166019.1, NP_001159491.1 |
| RefSeq ORF | 2760 bp |
| Locus ID | 162 |
| UniProt ID | Q10567 |
| Protein Pathways | Lysosome |
| MW | 101.1 kDa |
| Gene Summary | Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229039L3 | Lenti-ORF clone of AP1B1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 3 |
CNY 9,030.00 |
|
| RC229039L4 | Lenti-ORF clone of AP1B1 (mGFP-tagged)-Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 3 |
CNY 9,030.00 |
|
| RG229039 | AP1B1 (tGFP-tagged) - Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 3 |
CNY 7,890.00 |
|
| SC327674 | AP1B1 (untagged)-Human adaptor-related protein complex 1 beta 1 subunit (AP1B1) transcript variant 3 |
CNY 6,736.00 |
