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RASGRP3 (NM_015376) Human Tagged ORF Clone
CAT＃: RC228997
- TrueORF®
RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3
ORF Plasmid: tGFP
"NM_015376" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GRP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228997 representing NM_015376
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATTGAGATGTTTG ATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTGATGCACCGATGGTATTTATC TTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGAAATGCCACTGGAGAAAGCTGCAATGAATTT CGATTAAAGATCTGCTACTTCATGAGGTACTGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGATCTTG GTTTGATTCGTATGACTGAGGAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTCAGCCT CATCGACATATCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTATCCAAG AAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACTTTTCTGG AGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTCATCCATGGCTGCCTGGAGAA TAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGAATCTCTAAGTGGGTCCAGTTGATGGTTCTT AGCAAACCAACCCCCCAGCAAAGGGCAGAAGTCATCACAAAGTTTATCAATGTTGCAAAGAAGCTCCTTC AGCTCAAAAATTTTAACACCCTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATTTCACGCCTCAA AGAGACCCATTCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAGTTGGTCTCCTCC AACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATCCCCATCCTTGGAG TACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACAGAGGAGAACAAAGTGAACAT TGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTAGTCTCCCTGCAGAATGCCTCTCACCACTTA GAACCCAACATGGATTTGATCAACCTGCTCACGCTTTCCCTGGACCTCTATCACACTGAAGATGATATTT ACAAACTGTCACTGGTGCTGGAGCCTAGAAATTCTAAATCGCCTACCTCCCCTACGACGCCCAACAAGCC TGTGGTACCCCTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCCACGGTCATCAACAAGCACATA AGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGATGGGTACATTTCCCAAGAGGACT TTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTTCTGGACAAAGATCAGGATGGCCT AATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCTAAATCCCAACTACACTGTAAAATGGGACCA GGATTTATCCATAATTTTCAGGAGATGACCTATCTCAAGCCAACCTTCTGCGAACACTGTGCGGGATTTC TCTGGGGCATAATCAAGCAAGGATACAAATGCAAAGACTGTGGAGCCAATTGTCACAAACAGTGCAAAGA CCTCCTGGTTCTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCCTTGAGCAGTGGTCATGGGTCACTGCCT GGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAGTTCCCTGGAGTCACTGCTGGACACAGGG ATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCTCGCAAGATCTCTGTGAGGCTACAGAGGGC CACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCAGAGGCTGGCTGGGGGGACTCGGGGTCCCAC ACCTTCCCTAAAATGAAATCCAAGTTCCATGACAAAGCAGCAAAGGACAAAGGCTTTGCCAAATGGGAAA ATGAGAAGCCCAGGGTGCATGCTGGTGTGGATGTTGTAGACCGGGGCACGGAGTTTGAACTTGACCAGGA TGAAGGAGAAGAGACCAGACAGGATGGTGAGGATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228997 representing NM_015376
Red=Cloning site Green=Tags(s) MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRWYLSSTELAEKLLCMYRNATGESCNEF RLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSK KGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVL SKPTPQQRAEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTELVSS NGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLSVTLSELVSLQNASHHL EPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSPTSPTTPNKPVVPLEWALGVMPKPDPTVINKHI RKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGP GFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLP GSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAGWGDSGSH TFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEETRQDGEDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015376 |
| ORF Size | 2067 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015376.2, NP_056191.1 |
| RefSeq Size | 4353 bp |
| RefSeq ORF | 2070 bp |
| Locus ID | 25780 |
| UniProt ID | Q8IV61 |
| Domains | RasGEFN, RasGEF, EFh, DAG_PE-bind |
| Protein Pathways | B cell receptor signaling pathway, MAPK signaling pathway |
| MW | 78.2 kDa |
| Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228997L3 | Lenti-ORF clone of RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 5,890.00 |
|
| RC228997L4 | Lenti-ORF clone of RASGRP3 (mGFP-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 5,890.00 |
|
| RG228997 | RASGRP3 (tGFP-tagged) - Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 4,370.00 |
|
| SC327632 | RASGRP3 (untagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3) transcript variant 3 |
CNY 7,220.00 |
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