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Glycogen synthase 1 (GYS1) (NM_001161587) Human Tagged ORF Clone
CAT＃: RC228993
- TrueORF®
GYS1 (Myc-DDK-tagged)-Human glycogen synthase 1 (muscle) (GYS1), transcript variant 2
ORF Plasmid: tGFP
"NM_001161587" in other vectors (4)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GSY; GYS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228993 representing NM_001161587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTAAACCGCACTTTGTCCATGTCCTCACTGCCAGGACTGGAGGACTGGGAGGATGAATTCGACC TGGAGAACGCAGTGCTCTTCGAAGTGGCCTGGGAGGTGGCTAACAAGGTGGGTGGCATCTACACGGTGCT GCAGACGAAGGCGAAGGTGACAGGGGACGAATGGGGCGACAACTACTTCCTGGTGGGGCCGTACACGGAG CAGGGCGTGAGGACCCAGGTGGAACTGCTGGAGGCCCCCACCCCGGCCCTGAAGAGGACACTGGATTCCA TGAACAGCAAGGGCTGCAAGTTCCTGGCACAGAGTGAGGAGAAGCCACATGTGGTTGCTCACTTCCATGA GTGGTTGGCAGGCGTTGGACTCTGCCTGTGTCGTGCCCGGCGACTGCCTGTAGCAACCATCTTCACCACC CATGCCACGCTGCTGGGGCGCTACCTGTGTGCCGGTGCCGTGGACTTCTACAACAACCTGGAGAACTTCA ACGTGGACAAGGAAGCAGGGGAGAGGCAGATCTACCACCGATACTGCATGGAAAGGGCGGCAGCCCACTG CGCTCACGTCTTCACTACTGTGTCCCAGATCACCGCCATCGAGGCACAGCACTTGCTCAAGAGGAAACCA GATATTGTGACCCCCAATGGGCTGAATGTGAAGAAGTTTTCTGCCATGCATGAGTTCCAGAACCTCCATG CTCAGAGCAAGGCTCGAATCCAGGAGTTTGTGCGGGGCCATTTTTATGGGCATCTGGACTTCAACTTGGA CAAGACCTTATACTTCTTTATCGCCGGCCGCTATGAGTTCTCCAACAAGGGTGCTGACGTCTTCCTGGAG GCATTGGCTCGGCTCAACTATCTGCTCAGAGTGAACGGCAGCGAGCAGACAGTGGTTGCCTTCTTCATCA TGCCAGCGCGGACCAACAATTTCAACGTGGAAACCCTCAAAGGCCAAGCTGTGCGCAAACAGCTTTGGGA CACGGCCAACACGGTGAAGGAAAAGTTCGGGAGGAAGCTTTATGAATCCTTACTGGTTGGGAGCCTTCCC GACATGAACAAGATGCTGGATAAGGAAGACTTCACTATGATGAAGAGAGCCATCTTTGCAACGCAGCGGC AGTCTTTCCCCCCTGTGTGCACCCACAATATGCTGGATGACTCCTCAGACCCCATCCTGACCACCATCCG CCGAATCGGCCTCTTCAATAGCAGTGCCGACAGGGTGAAGGTGATTTTCCACCCGGAGTTCCTCTCCTCC ACAAGCCCCCTGCTCCCTGTGGACTATGAGGAGTTTGTCCGTGGCTGTCACCTTGGAGTCTTCCCCTCCT ACTATGAGCCTTGGGGCTACACACCGGCTGAGTGCACGGTTATGGGAATCCCCAGTATCTCCACCAATCT CTCCGGCTTCGGCTGCTTCATGGAGGAACACATCGCAGACCCCTCAGCTTACGGTATCTACATTCTTGAC CGGCGGTTCCGCAGCCTGGATGATTCCTGCTCGCAGCTCACCTCCTTCCTCTACAGTTTCTGTCAGCAGA GCCGGCGGCAGCGTATCATCCAGCGGAACCGCACGGAGCGCCTCTCCGACCTTCTGGACTGGAAATACCT AGGCCGGTACTATATGTCTGCGCGCCACATGGCGCTGTCCAAGGCCTTTCCAGAGCACTTCACCTACGAG CCCAACGAGGCGGATGCGGCCCAGGGGTACCGCTACCCACGGCCAGCCTCGGTGCCACCGTCGCCCTCGC TGTCACGACACTCCAGCCCGCACCAGAGTGAGGACGAGGAGGATCCCCGGAACGGGCCGCTGGAGGAAGA CGGCGAGCGCTACGATGAGGACGAGGAGGCCGCCAAGGACCGGCGCAACATCCGTGCACCAGAGTGGCCG CGCCGAGCGTCCTGCACCTCCTCCACCAGCGGCAGCAAGCGCAACTCTGTGGACACGGCCACCTCCAGCT CACTCAGCACCCCGAGCGAGCCCCTCAGCCCCACCAGCTCCCTGGGCGAGGAGCGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228993 representing NM_001161587
Red=Cloning site Green=Tags(s) MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNYFLVGPYTE QGVRTQVELLEAPTPALKRTLDSMNSKGCKFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTT HATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKP DIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEFSNKGADVFLE ALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKFGRKLYESLLVGSLP DMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSS TSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILD RRFRSLDDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYE PNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWP RRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161587 |
| ORF Size | 2019 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161587.2 |
| RefSeq ORF | 2022 bp |
| Locus ID | 2997 |
| UniProt ID | P13807 |
| Protein Pathways | Insulin signaling pathway, Starch and sucrose metabolism |
| MW | 76.3 kDa |
| Gene Summary | The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228993L3 | Lenti-ORF clone of GYS1 (Myc-DDK-tagged)-Human glycogen synthase 1 (muscle) (GYS1), transcript variant 2 |
CNY 8,650.00 |
|
| RC228993L4 | Lenti-ORF clone of GYS1 (mGFP-tagged)-Human glycogen synthase 1 (muscle) (GYS1), transcript variant 2 |
CNY 8,650.00 |
|
| RG228993 | GYS1 (tGFP-tagged) - Human glycogen synthase 1 (muscle) (GYS1), transcript variant 2 |
CNY 7,410.00 |
|
| SC327628 | GYS1 (untagged)-Human glycogen synthase 1 (muscle) (GYS1) transcript variant 2 |
CNY 5,432.00 |
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