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RASGRP 4 (RASGRP4) (NM_001146202) Human Tagged ORF Clone
CAT＃: RC228987
- TrueORF®
RASGRP4 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant b
ORF Plasmid: tGFP
"NM_001146202" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228987 representing NM_001146202
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAACAGGAGGGCGAGGCCGGC CCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGCAAGGTCATGGCTTCCATGAA CCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGAT TCAGCTGGCAGCCTGTGCCACGAGGACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGC CGTCCGCCGACCTGGCTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAG ACGGCTGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCCCAGAGAAGAC TGGGAGACTCTTCTGACCTCCCAGGCCTGGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTGGA GACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTCCGGTCCTTCCAGGCTATCACGCCCCAGGAC CTGCGGAGCTACGTTTTGCAGGGCTCAGTACGAGGCTGCCCGGCCCTGGAGGGCTCCGTAGGTCTCAGCA ACAGCGTGTCCCGCTGGGTGCAGGTGATGGTGCTGAGCCGTCCCGGGCCCCTACAGCGTGCACAGGTGCT GGACAAGTTCATTCACGTGGCACAGAGGCTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACA GGGGGCCTGTGTCACAGTGCCATCTCCAGACTCAAGGACTCCCATGCCCACCTGAGCCCTGACAGCACCA AGGCCCTCCTGGAGCTCACTGAGCTCCTTGCCTCCCACAACAACTACGCCCGCTACCGCCGCACCTGGGC TGGCTGCGCGGGTTTCCGGCTGCCTGTACTGGGCGTGCACCTCAAGGACCTGGTGTCCCTGCATGAGGCA CAGCCCGACAGGTTGCCTGACGGCCGCCTGCACCTACCCAAGCTGAACAACCTCTACCTGCGGCTGCAGG AGCTGGTGGCCCTCCAAGGGCAGCATCCACCCTGCAGCGCCAATGAGGATCTGCTGCACCTGCTCACGCT CTCCCTGGACCTCTTCTACACGGAAGACGAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCC AAGAGCCTGCCACCCTCCCCCTTCAATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGC CGGACAGGGTCACACTGGGTCGGCATGTGGAGCAGCTGGTGGAGTCTGTGTTCAAGAATTATGACCCTGA AGGCCGAGGAACAATCTCTCAGGAGGACTTTGAGCGACTCTCGGGCAATTTTCCCTTCGCCTGCCATGGG CTTCACCCACCCCCACGCCAGGGGAGAGGATCCTTCAGCAGAGAGGAGCTGACAGGGTACCTGCTCCGGG CCAGCGCCATCTGCTCCAAGTTGGGCCTGGCCTTCCTGCACACCTTCCATGAGGTCACCTTCCGAAAGCC TACCTTCTGCGACAGCTGCAGTGGCTTCCTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGC GGGCTGTGTTGCCACAAACACTGCAGAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGG GCGATGCAGGACCCCCCGGAGCTCCTGTCCCATCCACACCAGCTCCCCATGCCAGCTGTGGCTCCGAGGA AAATCACTCCTACACGCTATCCCTGGAGCCTGAGACTGGGTGCCAGCTTCGCCATGCCTGGACCCAGACT GAATCCCCACACCCTTCCTGGGAAACAGATACGGTCCCCTGCCCGGTGATGGACCCACCATCAACTGCAT CCTCCAAGCTGGATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228987 representing NM_001146202
Red=Cloning site Green=Tags(s) MNRKDSKRKSHQECTGKTGGRGRPRQVRRHKTCPSPREISKVMASMNLGLLSEGGCSEDELLEKCIQSFD SAGSLCHEDHMLNMVLAMHSWVLPSADLAARLLTSYQKATGDTQELRRLQICHLVRYWLMRHPEVMHQDP QLEEVIGRFWATVAREGNSAQRRLGDSSDLPGLGKKRKVSLLFDHLETGELAQHLTYLEFRSFQAITPQD LRSYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPLQRAQVLDKFIHVAQRLHQLQNFNTLMAVT GGLCHSAISRLKDSHAHLSPDSTKALLELTELLASHNNYARYRRTWAGCAGFRLPVLGVHLKDLVSLHEA QPDRLPDGRLHLPKLNNLYLRLQELVALQGQHPPCSANEDLLHLLTLSLDLFYTEDEIYELSYAREPRCP KSLPPSPFNAPLVVEWAPGVTPKPDRVTLGRHVEQLVESVFKNYDPEGRGTISQEDFERLSGNFPFACHG LHPPPRQGRGSFSREELTGYLLRASAICSKLGLAFLHTFHEVTFRKPTFCDSCSGFLWGVTKQGYRCREC GLCCHKHCRDQVKVECKKRPGAKGDAGPPGAPVPSTPAPHASCGSEENHSYTLSLEPETGCQLRHAWTQT ESPHPSWETDTVPCPVMDPPSTASSKLDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146202 |
| ORF Size | 1977 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146202.1, NP_001139674.1 |
| RefSeq ORF | 1980 bp |
| Locus ID | 115727 |
| UniProt ID | Q8TDF6 |
| Protein Families | Druggable Genome |
| Protein Pathways | MAPK signaling pathway |
| MW | 73.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228987L3 | Lenti-ORF clone of RASGRP4 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant b |
CNY 5,890.00 |
|
| RC228987L4 | Lenti-ORF clone of RASGRP4 (mGFP-tagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant b |
CNY 5,890.00 |
|
| RG228987 | RASGRP4 (tGFP-tagged) - Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant b |
CNY 4,370.00 |
|
| SC327622 | RASGRP4 (untagged)-Human RAS guanyl releasing protein 4 (RASGRP4) transcript variant b |
CNY 7,220.00 |
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