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CYP51A1 (NM_001146152) Human Tagged ORF Clone
CAT＃: RC228881
- TrueORF®
CYP51A1 (Myc-DDK-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2
ORF Plasmid: tGFP
"NM_001146152" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228881 representing NM_001146152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGGCAAGACATTTACTTACCTTCTGGGGAGTGATGCTGCTGCACTGCTTTTTAATAGTAAAAATG AAGACCTGAATGCAGAAGATGTCTACAGTCGCCTGACAACACCTGTGTTTGGGAAGGGAGTTGCATACGA TGTGCCTAATCCAGTTTTCTTGGAGCAGAAGAAAATGTTAAAAAGTGGCCTTAACATAGCCCACTTTAAA CAGCATGTTTCTATAATTGAAAAAGAAACAAAGGAATACTTTGAGAGTTGGGGAGAAAGTGGAGAAAAAA ATGTGTTTGAAGCTCTTTCTGAGCTCATAATTTTAACAGCTAGCCATTGTTTGCATGGAAAGGAAATCAG AAGTCAACTCAATGAAAAGGTAGCACAGCTGTATGCAGATTTGGATGGAGGTTTCAGCCATGCAGCCTGG CTCTTACCAGGTTGGCTGCCTTTGCCTAGTTTCAGACGCAGGGACAGAGCTCATCGGGAAATCAAGGATA TTTTCTATAAGGCAATCCAGAAACGCAGACAGTCTCAAGAAAAAATTGATGACATTCTCCAAACTTTACT AGATGCTACATACAAGGATGGGCGTCCTTTGACTGATGATGAAGTAGCAGGGATGCTTATTGGATTACTC TTGGCAGGGCAGCATACATCCTCAACTACTAGTGCTTGGATGGGCTTCTTTTTGGCCAGAGACAAAACAC TTCAAAAAAAATGTTATTTAGAACAGAAAACAGTCTGTGGAGAGAATCTGCCTCCTTTAACTTATGACCA GCTCAAGGATCTAAATTTACTTGATCGCTGTATAAAAGAAACATTAAGACTTAGACCTCCTATAATGATC ATGATGAGAATGGCCAGAACTCCTCAGACTGTGGCAGGGTATACCATTCCTCCAGGACATCAGGTGTGTG TTTCTCCCACTGTCAATCAAAGACTTAAAGACTCATGGGTAGAACGCCTGGACTTTAATCCTGATCGCTA CTTACAGGATAACCCAGCATCAGGGGAAAAGTTTGCCTATGTGCCATTTGGAGCTGGGCGTCATCGTTGT ATTGGGGAAAATTTTGCCTATGTTCAAATTAAGACAATTTGGTCCACTATGCTTCGTTTATATGAATTTG ATCTCATTGATGGATACTTTCCCACTGTGAATTATACAACTATGATTCACACCCCTGAAAACCCAGTTAT CCGTTACAAACGAAGATCAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228881 representing NM_001146152
Red=Cloning site Green=Tags(s) MVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFK QHVSIIEKETKEYFESWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAW LLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLL LAGQHTSSTTSAWMGFFLARDKTLQKKCYLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMI MMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRC IGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146152 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146152.1, NP_001139624.1 |
| RefSeq Size | 2934 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 1595 |
| UniProt ID | Q16850 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| MW | 46.3 kDa |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228881L3 | Lenti-ORF clone of CYP51A1 (Myc-DDK-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 5,890.00 |
|
| RC228881L4 | Lenti-ORF clone of CYP51A1 (mGFP-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 5,890.00 |
|
| RG228881 | CYP51A1 (tGFP-tagged) - Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 4,370.00 |
|
| SC327516 | CYP51A1 (untagged)-Human cytochrome P450 family 51 subfamily A polypeptide 1 (CYP51A1) transcript variant 2 |
CNY 7,220.00 |
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