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GLYCTK (NM_001144951) Human Tagged ORF Clone
CAT＃: RC227749
- TrueORF®
GLYCTK (Myc-DDK-tagged)-Human glycerate kinase (GLYCTK), transcript variant 2
ORF Plasmid: tGFP
"NM_001144951" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HBeAgBP4A; HBEBP2; HBEBP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227749 representing NM_001144951
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCCCTGCAGGTCCTGCCCCGCTTGGCCCGAGCCCCCTTGCATCCACTCCTCTGGCGGGGCT CAGTGGCCCGTCTGGCCAGCAGCATGGCCTTGGCAGAGCAGGCCAGGCAGCTGTTTGAGAGTGCTGTAGG TGCAGTGCTGCCGGGCCCCATGCTGCACCGGGCACTATCCTTGGACCCTGGTGGCAGACAGCTGAAGGTG CGGGACCGGAACTTTCAGCTGAGGCAAAACCTCTACCTGGTGGGCTTTGGCAAGGCTGTGCTGGGTATGG CAGCTGCAGCTGAGGAACTACTGGGCCAGCATCTTGTGCAGGGCGTGATCAGCGTTCCCAAGGGGATCCG TGCTGCCATGGAGCGTGCCGGCAAGCAGGAGATGCTGCTGAAGCCACATAGCCGTGTCCAGGTATTCGAG GGTGCGGAGGACAACCTCCCGGACCGCGATGCGCTGCGGGCTGCACTGGCCATCCAGCAACTGGCTGAGG GACTCACAGCTGATGACCTGCTGCTCGTGCTGATCTCAGGTGGTGAGCCTCATCCTGTCAGATGTGGTGG GGGACCCTGTGGAGGTGATTGCCAGTGGCCCCACCGTGGCCAGTTCCCACAATGTGCAAGATTGCCTGCA TATCCTCAATCGCTACGGCCTCCGTGCAGCCCTGCCACGTTCTGTGAAGACTGTGCTGTCTCGGGCCGAC TC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227749 representing NM_001144951
Red=Cloning site Green=Tags(s) MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKV RDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFE GAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGEPHPVRCGGGPCGGDCQWPHRGQFPQCARLPA YPQSLRPPCSPATFCEDCAVSGRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001144951 |
| ORF Size | 702 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001144951.1, NP_001138423.1 |
| RefSeq ORF | 705 bp |
| Locus ID | 132158 |
| UniProt ID | Q8IVS8 |
| Protein Families | Transcription Factors |
| Protein Pathways | Glycerolipid metabolism, Glycine, serine and threonine metabolism, Glyoxylate and dicarboxylate metabolism, Metabolic pathways |
| MW | 24.9 kDa |
| Gene Summary | This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227749L3 | Lenti-ORF clone of GLYCTK (Myc-DDK-tagged)-Human glycerate kinase (GLYCTK), transcript variant 2 |
CNY 5,890.00 |
|
| RC227749L4 | Lenti-ORF clone of GLYCTK (mGFP-tagged)-Human glycerate kinase (GLYCTK), transcript variant 2 |
CNY 5,890.00 |
|
| RG227749 | GLYCTK (tGFP-tagged) - Human glycerate kinase (GLYCTK), transcript variant 2 |
CNY 4,370.00 |
|
| SC325640 | GLYCTK (untagged)-Human glycerate kinase (GLYCTK), transcript variant 2 |
CNY 2,400.00 |
