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EHMT1/GLP (EHMT1) (NM_001145527) Human Tagged ORF Clone
CAT＃: RC227601
- TrueORF®
EHMT1 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2
ORF Plasmid: tGFP
"NM_001145527" in other vectors (6)
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CNY 8,872.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EHMT1-IT1; Eu-HMTase1; EUHMTASE1; FP13812; GLP; GLP1; KLEFS1; KMT1D |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227601 representing NM_001145527
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCCGATGCCGAGGCAGTTCCGGCGAGGGGGGAGCCTCAGCAGGATTGCTGTGTGAAAACCG AGCTGCTGGGAGAAGAGACACCTATGGCTGCCGATGAAGGCTCAGCAGAGAAACAGGCAGGAGAGGCCCA CATGGCTGCGGACGGTGAGACCAATGGGTCTTGTGAAAACAGCGATGCCAGCAGTCATGCAAATGCTGCA AAGCACACTCAGGACAGCGCAAGGGTCAACCCCCAGGATGGCACCAACACACTAACTCGGATAGCGGAAA ATGGGGTTTCAGAAAGAGACTCAGAAGCGGCGAAGCAAAACCACGTCACTGCCGACGACTTTGTGCAGAC TTCTGTCATCGGCAGCAACGGATACATCTTAAATAAGCCGGCCCTACAGGCACAGCCCTTGAGGACTACC AGCACTCTGGCCTCTTCGCTGCCTGGCCATGCTGCAAAAACCCTTCCTGGAGGGGCTGGCAAAGGCAGGA CTCCAAGCGCTTTTCCCCAGACGCCAGCCGCCCCACCAGCCACCCTTGGGGAGGGGAGTGCTGACACAGA GGACAGGAAGCTCCCGGCCCCTGGCGCCGACGTCAAGGTCCACAGGGCACGCAAGACCATGCCGAAGTCC GTCGTGGGCCTGCATGCAGCCAGTAAAGATCCCAGAGAAGTTCGAGAAGCTAGAGATCATAAGGAACCAA AAGAGGAGATCAACAAAAACATTTCTGACTTTGGACGACAGCAGCTTTTACCCCCCTTCCCATCCCTTCA TCAGTCGCTACCTCAGAACCAGTGCTACATGGCCACCACAAAATCACAGACAGCTTGCTTGCCTTTTGTT TTAGCAGCTGCAGTATCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGCCTGGTTCCTAAGAAAAAGA CCAAAGTATTAAAACAGAGGACGGTGATTGAGATGTTTAAGAGCATAACTCATTCCACTGTGGGTTCCAA GGGGGAGAAGGACCTGGGCGCCAGCAGCCTGCACGTGAATGGGGAGAGCCTGGAGATGGACTCGGATGAG GACGACTCAGAGGAGCTCGAGGAGGACGACGGCCATGGTGCAGAGCAGGCGGCCGCGTTCCCCACAGAGG ACAGCAGGACTTCCAAGGAGAGCATGTCGGAGGCTGATCGCGCCCAGAAGATGGACGGGGAGTCCGAGGA GGAGCAGGAGTCCGTGGACACCGGGGAGGAGGAGGAAGGCGGTGACGAGTCTGACCTGAGTTCGGAATCC AGCATTAAGAAGAAATTTCTCAAGAGGAAAGGAAAGACCGACAGTCCCTGGATCAAGCCAGCCAGGAAAA GGAGGCGGAGAAGTAGAAAGAAGCCCAGCGGTGCCCTCGGTTCTGAGTCGTATAAGTCATCTGCAGGAAG CGCTGAGCAGACGGCACCAGGAGACAGCACAGGGTACATGGAAGTTTCTCTGGACTCCCTGGATCTCCGA GTCAAAGGAATTCTGTCTTCACAAGCAGAAGGGTTGGCCAACGGTCCAGATGTGCTGGAGACAGACGGCC TCCAGGAAGTGCCTCTCTGCAGCTGCCGGATGGAAACACCGAAGAGTCGAGAGATCACCACACTGGCCAA CAACCAGTGCATGGCTACAGAGAGCGTGGACCATGAATTGGGCCGGTGCACAGACAGCGTGGTCAAGTAT GAGCTGATGCGCCCCTCCAACAAGGCCCCGCTCCTCGTGCTGTGTGAAGACCACCGGGGCCGCATGGTGA AGCACCAGTGCTGTCCTGGCTGTGGCTACTTCTGCACAGCGGGTAATTTTATGGAGTGTCAGCCCGAGAG CAGCATCTCTCACCGTTTCCACAAAGACTGTGCCTCTCGAGTCAATAACGCCAGCTATTGTCCCCACTGT GGGGAGGAGAGCTCCAAGGCCAAAGAGGTGACGATAGCTAAAGCAGACACCACCTCGACCGTGACACCAG TCCCCGGGCAGGAGAAGGGCTCGGCCCTGGAGGGCAGGGCCGACACCACAACGGGCAGTGCTGCCGGGCC ACCACTCTCGGAGGACGACAAGCTGCAGGGTGCAGCCTCCCACGTGCCCGAGGGCTTTGATCCAACGGGA CCTGCTGGGCTTGGGAGGCCAACTCCCGGCCTTTCCCAGGGACCAGGGAAGGAAACCTTGGAGAGCGCTC TCATCGCCCTCGACTCGGAAAAACCCAAGAAGCTTCGCTTCCACCCAAAGCAGCTGTACTTCTCCGCCAG GCAAGGGGAGCTTCAGAAGGTGCTCCTCATGCTGGTGGACGGAATTGACCCCAACTTCAAAATGGAGCAC CAGAATAAGCGCTCTCCACTGCACGCCGCGGCAGAGGCTGGACACGTGGACATCTGCCACATGCTGGTTC AGTTCTGCAGGCTGGGAAGCCCAAGGTCGAGGGGCTGCCTTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227601 representing NM_001145527
Red=Cloning site Green=Tags(s) MAAADAEAVPARGEPQQDCCVKTELLGEETPMAADEGSAEKQAGEAHMAADGETNGSCENSDASSHANAA KHTQDSARVNPQDGTNTLTRIAENGVSERDSEAAKQNHVTADDFVQTSVIGSNGYILNKPALQAQPLRTT STLASSLPGHAAKTLPGGAGKGRTPSAFPQTPAAPPATLGEGSADTEDRKLPAPGADVKVHRARKTMPKS VVGLHAASKDPREVREARDHKEPKEEINKNISDFGRQQLLPPFPSLHQSLPQNQCYMATTKSQTACLPFV LAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGSKGEKDLGASSLHVNGESLEMDSDE DDSEELEEDDGHGAEQAAAFPTEDSRTSKESMSEADRAQKMDGESEEEQESVDTGEEEEGGDESDLSSES SIKKKFLKRKGKTDSPWIKPARKRRRRSRKKPSGALGSESYKSSAGSAEQTAPGDSTGYMEVSLDSLDLR VKGILSSQAEGLANGPDVLETDGLQEVPLCSCRMETPKSREITTLANNQCMATESVDHELGRCTDSVVKY ELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHC GEESSKAKEVTIAKADTTSTVTPVPGQEKGSALEGRADTTTGSAAGPPLSEDDKLQGAASHVPEGFDPTG PAGLGRPTPGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEH QNKRSPLHAAAEAGHVDICHMLVQFCRLGSPRSRGCLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145527 |
| ORF Size | 2424 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145527.1, NP_001138999.1 |
| RefSeq ORF | 2427 bp |
| Locus ID | 79813 |
| UniProt ID | Q9H9B1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
| MW | 86.5 kDa |
| Gene Summary | The protein encoded by this gene is a histone methyltransferase that methylates the lysine-9 position of histone H3. This action marks the genomic region packaged with these methylated histones for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227601L1 | Lenti-ORF clone of EHMT1 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2 |
CNY 11,272.00 |
|
| RC227601L2 | Lenti-ORF clone of EHMT1 (mGFP-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2 |
CNY 7,700.00 |
|
| RC227601L3 | Lenti-ORF clone of EHMT1 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2 |
CNY 7,700.00 |
|
| RC227601L4 | Lenti-ORF clone of EHMT1 (mGFP-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2 |
CNY 7,700.00 |
|
| RG227601 | EHMT1 (tGFP-tagged) - Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2 |
CNY 6,370.00 |
|
| SC326500 | EHMT1 (untagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 2, mRNA |
CNY 9,480.00 |
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