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DET1 (NM_001144074) Human Tagged ORF Clone
CAT＃: RC227483
- TrueORF®
DET1 (Myc-DDK-tagged)-Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 2
ORF Plasmid: tGFP
"NM_001144074" in other vectors (4)
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CNY 5,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227483 representing NM_001144074
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCATCATGTTTCTACCATCAAGCCTCGAAGAATCCAAAACCAAAATGTCATTCACCGCTTGGAAC GCCGGCGGATCAGTTCAGGCAAGGCAGGTACCCACTGGCACCAAGTCCGAGTGTTCCATCAGAATGTCTT CCCCAACTTCACAGTTGTCAACGTTGAAAAGCCTCCTTGTTTCTTGCGTAAATTCTCACCTGATGGACGC TACTTTATTGCTTTTTCTTCAGACCAGACATCTCTTGAAATCTATGAGTACCAGGGCTGCCAGGCAGCAG AGGACCTACTGCAGGGATACGAAGGAGAAATCCTGTCCAATGGCAATGACCAGCGGTCAGTGAATATCCG GGGCCGGCTCTTTGAACGCTTTTTTGTCCTGCTGCACATTACCAATGTTGCGGCCAATGGTGAGCACCTG AACCGGGAGTGTAGTCTCTTCACTGATGACTGCCGCTGTGTCATCGTGGGCTCAGCTGCCTACCTCCCAG ATGAGCCTCACCCTCCATTTTTTGAGGTATATCGGAACAGTGAATCAGTGACCCCCAACCCACGGTCCCC TCTAGAAGACTATTCCCTCCATATCATTGACCTTCACACCGGCCGCTTATGTGATACACGCACGTTCAAG TGTGACAAGGTGGTCTTGTCACACAACCAAGGGCTGTACTTGTACAAAAACATCCTGGCCATCTTGTCTG TGCAACAACAGACCATCCATGTCTTCCAGGTGACTCCTGAAGGCACTTTCATTGATGTGCGGACCATTGG CCGCTTTTGCTATGAGGATGACCTGCTCACTGTGTCAGCTGTTTTCCCTGAGGTACAGCGGGACAGTCAG ACAGGCATGGCCAATCCCTTTAGGGATCCTTTCATCAATTCCCTCAAACACCGGTTGCTGGTATATTTGT GGCGCCGGGCAGAACAGGATGGTAGTGCAATGGCCAAGAGGCGCTTCTTCCAGTATTTTGACCAACTGCG GCAGCTGCGAATGTGGAAAATGCAGCTTCTGGATGAAAACCACCTGTTTATCAAGTACACTAGTGAGGAT GTAGTAACACTGCGAGTCACAGATCCATCACAGGCATCTTTCTTTGTGGTGTACAATATGGTGACGACAG AGGTGATTGCTGTGTTTGAGAATACATCAGATGAGCTTTTGGAGCTCTTTGAGAACTTCTGTGACCTTTT TCGTAATGCTACCCTGCACAGTGAAGTTCAGTTTCCCTGCTCAGCTTCTAGCAACAATTTTGCAAGGCAG ATCCAGCGCCGGTTCAAAGACACTATTATAAATGCCAAGTATGGAGGGCACACAGAGGCAGTACGCCGGC TGCTGGGTCAGCTCCCCATCAGTGCTCAGTCTTACAGCGGTAGCCCCTATCTGGATTTGTCTCTCTTCAG TTATGATGACAAGTGGGTATCTGTCATGGAGCGGCCCAAGACTTGTGGAGATCACCCAATCAGGTTCTAT GCCCGGGACTCGGGCCTGCTCAAGTTTGAGATCCAGGCGGGGTTATTGGGCCGCCCCATCAACCACACAG TGCGACGCCTTGTTGCCTTCACCTTTCACCCTTTTGAGCCTTTCGCTATTTCTGTGCAGAGGACTAATGC TGAGTATGTTGTCAACTTCCATATGCGACACTGCTGCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227483 representing NM_001144074
Red=Cloning site Green=Tags(s) MDHHVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGR YFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVNIRGRLFERFFVLLHITNVAANGEHL NRECSLFTDDCRCVIVGSAAYLPDEPHPPFFEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFK CDKVVLSHNQGLYLYKNILAILSVQQQTIHVFQVTPEGTFIDVRTIGRFCYEDDLLTVSAVFPEVQRDSQ TGMANPFRDPFINSLKHRLLVYLWRRAEQDGSAMAKRRFFQYFDQLRQLRMWKMQLLDENHLFIKYTSED VVTLRVTDPSQASFFVVYNMVTTEVIAVFENTSDELLELFENFCDLFRNATLHSEVQFPCSASSNNFARQ IQRRFKDTIINAKYGGHTEAVRRLLGQLPISAQSYSGSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRFY ARDSGLLKFEIQAGLLGRPINHTVRRLVAFTFHPFEPFAISVQRTNAEYVVNFHMRHCCT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001144074 |
| ORF Size | 1650 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001144074.3 |
| RefSeq ORF | 1653 bp |
| Locus ID | 55070 |
| UniProt ID | Q7L5Y6 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 63.7 kDa |
| Gene Summary | Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227483L3 | Lenti-ORF clone of DET1 (Myc-DDK-tagged)-Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 2 |
CNY 7,410.00 |
|
| RC227483L4 | Lenti-ORF clone of DET1 (mGFP-tagged)-Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 2 |
CNY 7,410.00 |
|
| RG227483 | DET1 (tGFP-tagged) - Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 2 |
CNY 6,080.00 |
|
| SC325191 | DET1 (untagged)-Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 2 |
CNY 7,220.00 |
