PCDH15 (NM_001142773) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDHR15; DFNB23; USH1F
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC227376 representing NM_001142773 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGACAGTTTTATCTCTGGACATGTTTAGCTTCAGGGATCATCCTGGGCTCTCTCTTTGAAATCT GCTTGGGCCAGTATGATGATGGTACAATTCTGGTGGACAACATGCTGATCAAAGGGACTGCTGGAGGACC AGACCCCACCATAGAACTTTCTTTAAAGGATAATGTGGATTACTGGGTGTTGATGGATCCTGTTAAGCAA ATGCTTTTCCTGAACAGCACCGGAAGAGTTCTGGATAGAGATCCACCGATGAACATACACTCCATTGTGG TGCAGGTCCAGTGCATCAACAAAAAAGTGGGCACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGA CAGGAATGACAACTCACCCACTTTCAAGCATGAAAGCTACTATGCCACAGTGAATGAGCTCACTCCAGTT GGTACCACAATATTCACAGGATTTTCAGGAGACAATGGAGCTACAGATATAGATGATGGACCAAATGGAC AGATAGAGTATGTTATTCAGTATAATCCAGATGATCCGACATCCAATGACACCTTTGAAATTCCCCTAAT GTTGACTGGAAATATAGTGTTAAGGAAGAGGCTCAACTATGAAGATAAGACTCGCTACTTTGTCATAATC CAAGCTAATGACCGTGCCCAAAATCTGAATGAGAGGCGAACCACCACCACCACTCTCACAGTGGATGTTC TGGATGGAGATGACTTGGGTCCAATGTTTCTTCCTTGTGTCCTTGTGCCAAACACTCGTGATTGCCGTCC ACTCACTTATCAAGCTGCCATACCTGAGTTGAGAACTCCGGAAGAACTGAACCCCATTATTGTTACGCCA CCAATCCAAGCCATTGATCAGGACCGGAATATTCAACCGCCATCAGATAGGCCAGGAATCCTCTATTCCA TCCTTGTTGGGACTCCTGAGGATTACCCACGATTTTTCCATATGCATCCTAGGACAGCAGAACTTAGTCT CCTGGAGCCAGTAAACAGAGACTTTCACCAGAAATTTGATTTGGTTATTAAGGCTGAACAAGACAATGGT CATCCTCTTCCTGCCTTTGCCGGTCTACACATTGAAATACTGGATGAAAACAATCAAAGTCCATATTTTA CAATGCCCAGTTATCAAGGCTATATCCTGGAATCTGCCCCAGTGGGAGCAACCATTTCGGACAGTCTCAA TTTGACTTCACCTTTAAGAATAGTAGCTCTGGACAAGGACATAGAAGATACAAAAGACCCAGAGCTTCAC CTTTTTCTGAATGACTACACCTCAGTCTTCACCGTCACACAGACTGGTATTACTCGCTACCTCACCTTAC TTCAACCAGTGGACAGGGAAGAACAGCAAACTTACACCTTTTCGATAACAGCATTTGATGGTGTACAAGA AAGTGAGCCAGTCATCGTCAATATTCAAGTGATGGATGCAAATGATAACACGCCAACCTTCCCTGAAATA TCCTATGATGTGTATGTTTATACAGACATGAGACCTGGGGACAGTGTCATACAGCTCACTGCAGTCGACG CAGACGAAGGGTCAAATGGGGAGATCACATATGAAATCCTTGTTGGGGCTCAGGGAGACTTCATCATCAA TAAAACAACAGGGCTTATCACCATCGCTCCAGGGGTGGAAATGATAGTCGGGCGGACTTACGCACTCACG GTCCAAGCAGCGGATAATGCTCCTCCTGCAGAGCGAAGGAACTCCATCTGCACTGTGTATATTGAAGTGC TTCCACCAAATAATCAAAGCCCTCCTCGCTTCCCACAGCTGATGTATAGCCTTGAAATTAGTGAAGCCAT GAGGGTTGGTGCTGTTTTATTAAATCTACAGGCAACTGATCGAGAGGGAGACTCAATAACATATGCCATT GAGAATGGAGATCCTCAGAGAGTTTTTAATCTTTCAGAAACCACGGGGATTCTAACCTTAGGGAAAGCAC TGGACAGGGAAAGCACTGATCGCTACATTCTGATCATCACAGCTTCAGATGGCAGGCCAGATGGGACCTC AACTGCCACAGTAAACATAGTGGTGACAGATGTCAATGACAATGCTCCAGTGTTTGATCCTTATCTGCCA AGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGTCAAGTAAAAGCAACAGACCCTGATG CTGGAATAAATGGTCAAGTGCACTACAGTTTGGGTAACTTTAATAATCTTTTTCGTATCACATCCAATGG GAGCATTTACACAGCAGTGAAGCTTAACAGAGAAGTCAGGGACTACTATGAACTTGTTGTTGTGGCAACA GATGGAGCAGTACACCCTCGTCATTCAACTCTAACCTTGGCCATCAAGGTTTTGGACATTGATGATAACA GTCCTGTGTTCACCAATTCAACATACACTGTCCTTGTTGAAGAGAATTTGCCAGCTGGGACTACCATCCT TCAAATAGAGGCCAAAGATGTCGACCTTGGAGCAAATGTGTCTTACCGGATAAGAAGCCCAGAAGTGAAG CACTTTTTTGCACTACATCCATTTACAGGAGAACTATCGCTTTTAAGGAGTTTAGATTATGAGGCATTTC CAGACCAAGAAGCAAGTATCACTTTTCTGGTAGAGGCCTTTGATATTTATGGAACAATGCCACCTGGTAT TGCTACTGTCACAGTGATTGTAAAGGATATGAATGATTATCCTCCTGTCTTTAGTAAACGAATATACAAA GGGATGGTGGCTCCGGATGCAGTCAAGGGTACACCTATCACAACAGTTTATGCTGAAGATGCAGACCCTC CTGGATTACCTGCAAGTCGTGTGAGGTATAGAGTAGATGATGTACAGTTTCCTTACCCTGCCAGTATTTT TGAAGTGGAAGAAGATTCTGGAAGAGTAATAACACGAGTCAATCTTAATGAAGAACCTACAACAATTTTT AAGTTGGTGGTGGTTGCTTTTGATGATGGGGAGCCTGTGATGTCCAGCAGTGCCACAGTGAAGATTCTTG TCTTACATCCTGGTGAGATCCCACGCTTCACACAGGAGGAATATAGACCTCCTCCAGTAAGTGAACTTGC CACCAAAGGGACCATGGTTGGTGTAATTTCTGCTGCTGCCATTAATCAAAGTATTGTGTACTCCATTGTT TCAGGAAATGAAGAAGATACATTTGGAATTAATAACATCACAGGTGTTATCTATGTGAATGGACCTCTGG ATTATGAGACCAGGACAAGCTATGTACTTCGAGTCCAAGCTGATTCCCTGGAAGTGGTCCTTGCCAATCT CCGAGTTCCTTCAAAAAGCAATACAGCTAAAGTATACATTGAGATTCAGGATGAAAATAATCATCCCCCA GTGTTTCAGAAAAAATTCTACATCGGAGGTGTATCTGAAGATGCAAGAATGTTTACTTCTGTACTCAGAG TGAAGGCTACTGATAAAGATACTGGCAATTATAGTGTCATGGCCTACAGACTCATAATACCACCAATTAA AGAGGGAAAAGAAGGATTTGTAGTGGAAACATATACAGGGCTTATCAAAACTGCTATGCTCTTCCATAAT ATGAGGAGATCCTACTTCAAGTTTCAAGTTATTGCAACTGACGACTATGGGAAGGGACTGAGCGGCAAAG CCGATGTACTCGTCTCCGTGGTCAATCAGCTGGATATGCAAGTCATTGTTTCCAATGTGCCTCCTACTCT AGTGGAAAAAAAGATAGAAGATCTTACAGAGATCTTGGATCGCTATGTTCAGGAACAAATTCCTGGTGCC AAGGTCGTAGTGGAGTCCATTGGAGCTCGCCGGCATGGAGATGCCTTTTCCCTAGAAGATTACACCAAAT GTGACTTGACTGTCTATGCAATTGACCCCCAAACCAACAGAGCCATCGATAGAAATGAGCTTTTTAAATT TTTGGATGGCAAACTACTTGATATCAATAAAGACTTTCAGCCGTATTATGGGGAAGGAGGACGCATTCTG GAGATCCGGACTCCAGAGGCAGTGACCAGCATTAAAAAGAGAGGAGAAAGTCTAGGATACACAGAAGGGG CCTTGTTGGCTCTGGCCTTCATCATCATCCTCTGCTGCATTCCTGCCATCTTGGTGGTTTTGGTCAGCTA CAGACAACGTCAAGCTGAGTGTACAAAGACTGCACGAATTCAGGCCGCATTACCCGCGGCTAAACCAGCA GTGCCGGCTCCTGCACCAGTGGCAGCGCCCCCGCCGCCGCCGCCGCCTCCGCCAGGTGCGCATCTCTATG AAGAACTTGGAGACAGCTCAATGCATAATCTTTTCCTTCTCTACCATTTTCAACAAAGCAGGGGAAATAA CTCAGTCTCAGAAGACAGGAAACATCAACAAGTTGTGATGCCCTTTTCTTCCAATACTATTGAGGCTCAC AAGTCAGCTCATGTAGACGGATCACTTAAGAGCAACAAACTGAAGTCTGCAAGAAAATTCACATTTCTAT CTGATGAGGATGACTTAAGTGCCCATAATCCCCTTTATAAGGAAAACATAAGTCAAGTATCAACAAATTC AGACATTTCACAGAGAACAGATTTTGTAGACCCATTTTCACCCAAAATACAAGCCAAGAGTAAGTCTCTG AGGGGCCCAAGAGAAAAGATTCAGAGGCTGTGGAGTCAGTCAGTCAGCTTACCCAGGAGGCTGATGAGGA AAGTTCCAAATAGACCAGAGATCATAGATCTGCAGCAGTGGCAAGGCACCAGGCAGAAAGCTGAAAATGA AAACACTGGAATCTGTACAAACAAAAGAGGTAGCAGCAATCCATTGCTTACAACTGAAGAGGCAAATTTG ACAGAGAAAGAGGAAATAAGGCAAGGTGAAACACTGATGATAGAAGGAACAGAACAGTTGAAATCTCTCT CTTCAGACTCTTCATTTTGCTTTCCCAGGCCTCACTTCTCATTCTCCACTTTGCCAACTGTTTCAAGAAC TGTGGAACTCAAATCAGAACCTAATGTCATCAGTTCTCCTGCTGAGTGTTCCTTGGAACTTTCTCCTTCA AGGCCTTGTGTTTTACATTCTTCACTCTCTAGGAGAGAGACACCTATTTGTATGTTACCTATTGAAACCG AAAGAAATATTTTTGAAAATTTTGCCCATCCACCAAACATCTCTCCTTCTGCCTGTCCCCTTCCCCCTCC TCCTCCTATTTCTCCTCCTTCTCCTCCTCCTGCTCCTGCTCCTCTTGCTCCTCCTCCTGACATTTCTCCT TTTTCTCTTTTTTGTCCTCCTCCCTCTCCTCCTTCTATCCCTCTTCCTCTTCCTCCTCCTACATTTTTTC CACTTTCCGTTTCAACGTCTGGTCCCCCAACACCACCTCTTCTACCTCCATTTCCAACTCCTCTTCCTCC ACCACCTCCTTCTATTCCTTGCCCTCCACCTCCTTCAGCTTCATTTCTGTCCACAGAGTGTGTCTGTATA ACAGGTGTTAAATGCACGACCAACTTGATGCCTGCCGAGAAAATTAAGTCCTCTATGACACAGCTATCAA CAACGACAGTGTGTAAAACAGACCCTCAGAGAGAACCAAAAGGCATCCTCAGACACGTTAAAAACTTAGC AGAACTTGAAAAATCAGTAGCTAACATGTACAGTCAAATAGAAAAAAACTATCTACGCACAAATGTTTCA GAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATATGGAAATCACATCTGAACAAAACAAGGGGAGTT TGAACAATATTGTCGAGGGAACTGAAAAACAATCTCACAGTCAATCTACTTCACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC227376 representing NM_001142773 Red=Cloning site Green=Tags(s) MFRQFYLWTCLASGIILGSLFEICLGQYDDGTILVDNMLIKGTAGGPDPTIELSLKDNVDYWVLMDPVKQ MLFLNSTGRVLDRDPPMNIHSIVVQVQCINKKVGTIIYHEVRIVVRDRNDNSPTFKHESYYATVNELTPV GTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPTSNDTFEIPLMLTGNIVLRKRLNYEDKTRYFVII QANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLTYQAAIPELRTPEELNPIIVTP PIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTAELSLLEPVNRDFHQKFDLVIKAEQDNG HPLPAFAGLHIEILDENNQSPYFTMPSYQGYILESAPVGATISDSLNLTSPLRIVALDKDIEDTKDPELH LFLNDYTSVFTVTQTGITRYLTLLQPVDREEQQTYTFSITAFDGVQESEPVIVNIQVMDANDNTPTFPEI SYDVYVYTDMRPGDSVIQLTAVDADEGSNGEITYEILVGAQGDFIINKTTGLITIAPGVEMIVGRTYALT VQAADNAPPAERRNSICTVYIEVLPPNNQSPPRFPQLMYSLEISEAMRVGAVLLNLQATDREGDSITYAI ENGDPQRVFNLSETTGILTLGKALDRESTDRYILIITASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLP RNLSVVEEEANAFVGQVKATDPDAGINGQVHYSLGNFNNLFRITSNGSIYTAVKLNREVRDYYELVVVAT DGAVHPRHSTLTLAIKVLDIDDNSPVFTNSTYTVLVEENLPAGTTILQIEAKDVDLGANVSYRIRSPEVK HFFALHPFTGELSLLRSLDYEAFPDQEASITFLVEAFDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYK GMVAPDAVKGTPITTVYAEDADPPGLPASRVRYRVDDVQFPYPASIFEVEEDSGRVITRVNLNEEPTTIF KLVVVAFDDGEPVMSSSATVKILVLHPGEIPRFTQEEYRPPPVSELATKGTMVGVISAAAINQSIVYSIV SGNEEDTFGINNITGVIYVNGPLDYETRTSYVLRVQADSLEVVLANLRVPSKSNTAKVYIEIQDENNHPP VFQKKFYIGGVSEDARMFTSVLRVKATDKDTGNYSVMAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHN MRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDMQVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGA KVVVESIGARRHGDAFSLEDYTKCDLTVYAIDPQTNRAIDRNELFKFLDGKLLDINKDFQPYYGEGGRIL EIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCCIPAILVVLVSYRQRQAECTKTARIQAALPAAKPA VPAPAPVAAPPPPPPPPPGAHLYEELGDSSMHNLFLLYHFQQSRGNNSVSEDRKHQQVVMPFSSNTIEAH KSAHVDGSLKSNKLKSARKFTFLSDEDDLSAHNPLYKENISQVSTNSDISQRTDFVDPFSPKIQAKSKSL RGPREKIQRLWSQSVSLPRRLMRKVPNRPEIIDLQQWQGTRQKAENENTGICTNKRGSSNPLLTTEEANL TEKEEIRQGETLMIEGTEQLKSLSSDSSFCFPRPHFSFSTLPTVSRTVELKSEPNVISSPAECSLELSPS RPCVLHSSLSRRETPICMLPIETERNIFENFAHPPNISPSACPLPPPPPISPPSPPPAPAPLAPPPDISP FSLFCPPPSPPSIPLPLPPPTFFPLSVSTSGPPTPPLLPPFPTPLPPPPPSIPCPPPPSASFLSTECVCI TGVKCTTNLMPAEKIKSSMTQLSTTTVCKTDPQREPKGILRHVKNLAELEKSVANMYSQIEKNYLRTNVS ELQTMCPSEVTNMEITSEQNKGSLNNIVEGTEKQSHSQSTSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001142773
ORF Size	5796 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001142773.2
RefSeq ORF	5799 bp
Locus ID	65217
Protein Families	Druggable Genome, Transmembrane
MW	213.67 kDa
Gene Summary	This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]

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