SETD1B (NM_015048) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KMT2G; Set1B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC227091 representing NM_015048 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACAGTCACCCCCCCCACCACCACCACCAGCAGCCCCCGCCGCAGCCCGGCCCTTCGGGCGAGA GGAGGAACCACCATTGGAGAAGTTACAAGTTGATGATTGACCCGGCTCTGAAAAAGGGGCATCATAAACT GTACCGCTACGATGGGCAGCATTTCAGCCTGGCGATGTCCAGCAACCGCCCGGTGGAAATTGTCGAAGAT CCCCGGGTCGTCGGGATCTGGACCAAAAACAAGGAGCTGGAGCTGTCGGTGCCCAAATTCAAGATCGATG AGTTCTACGTGGGCCCGGTGCCTCCGAAGCAGGTGACATTTGCCAAGCTGAATGATAACATCCGTGAAAA CTTCCTGAGGGACATGTGCAAGAAGTATGGGGAGGTGGAGGAGGTGGAGATTTTGTACAACCCCAAGACC AAGAAGCACCTGGGCATCGCCAAGGTGGTCTTTGCCACGGTCCGGGGAGCCAAGGATGCCGTTCAGCACT TGCACAGCACTTCCGTCATGGGCAACATTATCCACGTGGAGCTGGACACCAAAGGGGAAACCCGAATGCG GTTCTATGAACTGTTGGTCACTGGCCGATACACCCCCCAGACCCTCCCAGTGGGCGAGCTGGACGCTGTC TCTCCAATCGTGAATGAGACCCTGCAGCTGTCAGATGCCCTGAAGCGCCTCAAGGATGGAGGCCTGTCTG CAGGCTGTGGCTCCGGCTCCTCCTCTGTCACCCCCAATAGCGGTGGGACACCCTTCTCCCAGGACACAGC TTATTCCAGCTGCCGCCTGGACACACCCAACTCCTATGGACAGGGCACCCCGCTCACACCGCGCCTGGGC ACCCCTTTCTCACAGGACTCCAGCTACTCCAGCCGCCAGCCCACACCCTCATACCTCTTCAGCCAGGACC CTGCAGTGACCTTCAAGGCCCGGCGCCACGAGAGCAAGTTCACGGACGCCTACAACCGCCGCCACGAACA TCATTATGTACACAATTCTCCCGCGGTCACTGCGGTGGCCGGGGCCACAGCCGCTTTCCGGGGTTCCTCG GACCTCCCGTTCGGAGCAGTCGGCGGCACTGGGGGCAGCAGCGGTCCCCCGTTCAAGGCTCAACCACAGG ATTCAGCCACATTTGCCCACACTCCACCACCCGCCCAAGCAACCCCTGCTCCTGGATTCAAGTCTGCTTT CTCTCCGTATCAGACCCCAGTGGCCCACTTCCCTCCACCCCCGGAAGAGCCCACCGCCACAGCCGCTTTT GGGGCCCGCGACAGTGGGGAGTTCCGGAGGGCACCGGCGCCCCCACCCCTGCCACCTGCTGAGCCTCTGG CCAAGGAGAAGCCAGGCACGCCACCCGGCCCGCCGCCCCCCGACACCAACAGCATGGAGCTGGGCGGCCG GCCCACCTTCGGCTGGAGTCCTGAGCCCTGTGACAGCCCTGGCACGCCCACGCTGGAGTCGTCCCCTGCA GGGCCAGAGAAACCCCACGACAGCCTGGACTCGCGCATCGAGATGCTGCTGAAGGAGCAGCGCACCAAGC TGCTCTTCCTGAGGGAGCCGGACTCGGACACCGAGCTGCAGATGGAGGGCAGCCCCATCTCCTCCTCCTC CTCCCAGCTCTCCCCACTGGCCCCCTTTGGCACCAACTCCCAGCCAGGCTTCCGGGGCCCCACGCCCCCC TCGTCACGCCCCTCCAGCACCGGCCTGGAGGATATCAGCCCAACACCCCTCCCAGACTCCGACGAGGACG AGGAGCTCGACCTGGGCCTTGGGCCTCGGCCTCCACCTGAGCCAGGCCCCCCGGACCCTGCTGGGCTTCT GAGCCAGACAGCTGAGGTGGCCTTGGACCTGGTTGGAGACAGAACCCCGACCTCAGAGAAGATGGATGAG GGCCAGCAGTCCTCAGGCGAGGACATGGAGATCTCGGATGACGAGATGCCCTCGGCCCCCATCACCAGCG CTGACTGCCCCAAGCCCATGGTGGTGACCCCAGGAGCGGCAGCCGTGGCAGCCCCTTCTGTGCTAGCCCC AACCCTGCCGCTGCCCCCGCCACCTGGCTTCCCCCCGCTGCCCCCCCCACCACCACCACCCCCACCGCAG CCTGGCTTCCCCATGCCCCCACCGCTGCCCCCACCGCCGCCCCCACCCCCTCCAGCCCACCCTGCTGTGA CAGTGCCCCCACCACCCTTGCCAGCGCCGCCTGGAGTCCCGCCCCCACCCATCCTGCCACCACTGCCCCC CTTTCCGCCGGGCCTGTTCCCTGTGATGCAGGTGGACATGAGCCACGTGCTGGGTGGCCAGTGGGGCGGC ATGCCCATGTCCTTCCAGATGCAAACGCAGGTGCTCAGCCGGCTGATGACGGGCCAGGGCGCCTGCCCCT ACCCGCCCTTCATGGCCGCTGCGGCCGCCGCTGCCTCAGCTGGGCTCCAGTTTGTCAACCTGCCGCCCTA CCGGGGCCCCTTCTCCCTGAGCAACTCCGGCCCAGGCCGCGGGCAGCACTGGCCACCACTGCCCAAGTTT GACCCGTCAGTGCCTCCACCAGGCTACATGCCACGCCAGGAGGACCCACACAAAGCCACGGTGGATGGCG TCCTGCTGGTGGTCCTCAAAGAACTCAAGGCCATCATGAAGCGTGACCTGAACCGCAAGATGGTGGAAGT GGTGGCTTTCCGGGCCTTTGACGAGTGGTGGGACAAGAAGGAGCGGATGGCCAAGGCCTCGCTGACCCCG GTGAAGTCGGGCGAGCACAAGGACGAGGACAGGCCGAAGCCCAAGGACCGCATCGCCTCGTGCCTGCTGG AGTCATGGGGCAAGGGCGAGGGCCTGGGCTACGAGGGCCTGGGCCTGGGCATTGGGCTGCGTGGGGCCAT TCGCCTGCCCTCCTTCAAGGTCAAGAGGAAGGAGCCACCAGACACCACCTCATCTGGCGACCAGAAGCGG CTGCGGCCCTCGACCTCTGTGGATGAGGAAGATGAAGAGTCCGAGCGAGAGCGAGACCGGGATATGGCAG ACACCCCCTGTGAGCTCGCCAAGCGGGACCCCAAGGGCGTGGGTGTGCGGCGGCGGCCGGCGCGGCCTCT GGAGCTGGACAGTGGTGGGGAGGAGGACGAGAAGGAGTCATTGTCGGAGGAACAGGAGAGCACCGAGGAG GAAGAGGAGGCGGAGGAGGAGGAGGAGGAGGAAGATGACGACGATGACGACAGTGATGACCGGGACGAGT CTGAGAACGATGACGAGGACACAGCCCTGTCAGAGGCGAGTGAGAAGGACGAAGGGGACTCGGATGAAGA GGAGACAGTGAGCATTGTAACCTCCAAGGCCGAAGCCACGTCGTCCAGTGAGAGTTCCGAGTCTTCTGAG TTTGAGTCAAGCTCCGAGTCCTCGCCCTCATCCTCGGAGGATGAGGAGGAGGTAGTGGCCAGGGAAGAGG AGGAAGAAGAGGAGGAGGAGGAGATGGTGGCCGAGGAAAGCATGGCTTCTGCAGGCCCTGAGGACTTTGA GCAGGACGGGGAGGAAGCGGCTCTGGCCCCGGGGGCACCTGCAGTGGACTCGTTGGGCATGGAAGAGGAG GTGGACATCGAGACTGAGGCTGTGGCCCCTGAGGAGCGGCCCTCCATGCTGGACGAGCCCCCCTTGCCTG TGGGTGTTGAAGAGCCAGCGGACTCCAGGGAGCCGCCTGAGGAACCAGGCCTGAGCCAGGAAGGGGCCAT GTTGCTGTCTCCAGAGCCCCCTGCCAAGGAGGTGGAGGCTCGACCCCCATTGTCCCCTGAGCGAGCTCCA GAACATGACCTGGAAGTGGAGCCGGAGCCCCCTATGATGCTCCCCTTGCCGCTGCAACCACCATTGCCGC CCCCACGACCACCCCGGCCACCCAGCCCACCGCCGGAGCCTGAGACCACAGATGCCTCACACCCATCTGT CCCTCCGGAGCCCCTTGCCGAGGACCACCCCCCGCATACTCCAGGCCTCTGTGGCAGCCTGGCCAAGTCG CAGAGCACAGAGACGGTGCCAGCCACACCAGGCGGGGAGCCCCCGCTATCAGGGGGCAGCAGTGGCCTGT CCCTGAGCTCTCCGCAAGTGCCCGGCAGCCCCTTCTCCTACCCAGCCCCGTCCCCTAGCTTGAGCAGTGG GGGCCTCCCTCGGACACCTGGCCGGGACTTCAGCTTCACACCCACCTTCTCCGAGCCCAGCGGGCCCTTG CTCCTGCCCGTCTGCCCACTCCCCACTGGCCGACGCGATGAACGCTCCGGGCCCCTGGCCTCCCCGGTGC TCCTGGAGACGGGCCTGCCCCTCCCTCTGCCCCTTCCCCTGCCCTTGCCCTTGGCATTGCCCGCCGTCTT GCGGGCCCAGGCTCGTGCGCCCACCCCGCTGCCACCCCTGCTGCCCGCCCCCCTGGCCTCTTGCCCTCCC CCAATGAAGAGGAAGCCGGGCCGGCCCCGGCGATCCCCACCATCTATGCTCTCCTTGGATGGGCCCTTGG TCCGACCACCAGCAGGGGCCGCCCTTGGAAGGGAACTCCTGCTCCTGCCGGGCCAGCCACAGACCCCCGT CTTCCCCAGCACCCATGACCCCCGGACGGTGACCCTGGACTTCCGGAACGCGGGGATCCCAGCCCCTCCA CCACCCCTTCCCCCCCAGCCACCCCCACCCCCACCTCCCCCACCTGTAGAGCCCACCAAGCTGCCCTTTA AGGAGCTAGACAACCAGTGGCCCTCCGAGGCCATTCCTCCGGGCCCCCGTGGGCGCGATGAGGTCACTGA GGAATACATGGAGTTGGCCAAGAGCCGGGGGCCGTGGCGCCGGCCACCTAAGAAGCGCCATGAGGACCTG GTGCCACCTGCGGGCTCGCCCGAACTCTCGCCACCCCAGCCCCTCTTCCGGCCCCGCTCGGAGTTTGAGG AGATGACCATCCTGTATGACATCTGGAACGGTGGCATCGATGAGGAGGACATCCGCTTCCTGTGTGTCAC CTACGAGCGACTGCTACAGCAGGACAATGGCATGGACTGGCTTAACGACACGCTCTGGGTCTACCATCCC TCCACCAGCCTCTCTTCAGCTAAGAAGAAGAAACGGGACGATGGCATCCGCGAGCACGTGACGGGCTGTG CCCGCAGTGAGGGCTTCTACACCATCGACAAGAAGGACAAGCTCAGATACCTCAACAGCAGCCGTGCCAG CACCGATGAGCCCCCCGCAGACACCCAGGGCATGAGCATCCCAGCACAGCCCCACGCCTCCACCCGGGCA GGCTCGGAGCGGCGTTCGGAGCAGCGCCGCCTGCTGTCCTCCTTCACTGGCAGCTGTGACAGTGACCTGC TCAAGTTCAACCAGCTCAAGTTCCGGAAGAAAAAGCTCAAGTTCTGCAAGAGCCACATTCACGACTGGGG CTTGTTCGCCATGGAGCCCATCGCGGCTGACGAGATGGTCATCGAGTACGTGGGCCAGAATATCCGTCAG GTGATCGCAGACATGCGGGAGAAGCGTTATGAGGACGAGGGCATCGGGAGCAGCTACATGTTCCGGGTGG ACCATGACACCATCATCGACGCCACCAAGTGCGGCAACTTCGCGCGCTTCATCAACCACAGCTGCAACCC CAACTGCTATGCCAAGGTGATCACGGTGGAGTCACAGAAGAAGATAGTCATCTACTCGAAGCAGCACATT AACGTCAATGAGGAGATTACCTATGACTATAAGTTCCCCATCGAGGACGTCAAGATCCCCTGCCTCTGTG GCTCCGAGAACTGCCGGGGGACCCTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC227091 representing NM_015048 Red=Cloning site Green=Tags(s) MENSHPPHHHHQQPPPQPGPSGERRNHHWRSYKLMIDPALKKGHHKLYRYDGQHFSLAMSSNRPVEIVED PRVVGIWTKNKELELSVPKFKIDEFYVGPVPPKQVTFAKLNDNIRENFLRDMCKKYGEVEEVEILYNPKT KKHLGIAKVVFATVRGAKDAVQHLHSTSVMGNIIHVELDTKGETRMRFYELLVTGRYTPQTLPVGELDAV SPIVNETLQLSDALKRLKDGGLSAGCGSGSSSVTPNSGGTPFSQDTAYSSCRLDTPNSYGQGTPLTPRLG TPFSQDSSYSSRQPTPSYLFSQDPAVTFKARRHESKFTDAYNRRHEHHYVHNSPAVTAVAGATAAFRGSS DLPFGAVGGTGGSSGPPFKAQPQDSATFAHTPPPAQATPAPGFKSAFSPYQTPVAHFPPPPEEPTATAAF GARDSGEFRRAPAPPPLPPAEPLAKEKPGTPPGPPPPDTNSMELGGRPTFGWSPEPCDSPGTPTLESSPA GPEKPHDSLDSRIEMLLKEQRTKLLFLREPDSDTELQMEGSPISSSSSQLSPLAPFGTNSQPGFRGPTPP SSRPSSTGLEDISPTPLPDSDEDEELDLGLGPRPPPEPGPPDPAGLLSQTAEVALDLVGDRTPTSEKMDE GQQSSGEDMEISDDEMPSAPITSADCPKPMVVTPGAAAVAAPSVLAPTLPLPPPPGFPPLPPPPPPPPPQ PGFPMPPPLPPPPPPPPPAHPAVTVPPPPLPAPPGVPPPPILPPLPPFPPGLFPVMQVDMSHVLGGQWGG MPMSFQMQTQVLSRLMTGQGACPYPPFMAAAAAAASAGLQFVNLPPYRGPFSLSNSGPGRGQHWPPLPKF DPSVPPPGYMPRQEDPHKATVDGVLLVVLKELKAIMKRDLNRKMVEVVAFRAFDEWWDKKERMAKASLTP VKSGEHKDEDRPKPKDRIASCLLESWGKGEGLGYEGLGLGIGLRGAIRLPSFKVKRKEPPDTTSSGDQKR LRPSTSVDEEDEESERERDRDMADTPCELAKRDPKGVGVRRRPARPLELDSGGEEDEKESLSEEQESTEE EEEAEEEEEEEDDDDDDSDDRDESENDDEDTALSEASEKDEGDSDEEETVSIVTSKAEATSSSESSESSE FESSSESSPSSSEDEEEVVAREEEEEEEEEEMVAEESMASAGPEDFEQDGEEAALAPGAPAVDSLGMEEE VDIETEAVAPEERPSMLDEPPLPVGVEEPADSREPPEEPGLSQEGAMLLSPEPPAKEVEARPPLSPERAP EHDLEVEPEPPMMLPLPLQPPLPPPRPPRPPSPPPEPETTDASHPSVPPEPLAEDHPPHTPGLCGSLAKS QSTETVPATPGGEPPLSGGSSGLSLSSPQVPGSPFSYPAPSPSLSSGGLPRTPGRDFSFTPTFSEPSGPL LLPVCPLPTGRRDERSGPLASPVLLETGLPLPLPLPLPLPLALPAVLRAQARAPTPLPPLLPAPLASCPP PMKRKPGRPRRSPPSMLSLDGPLVRPPAGAALGRELLLLPGQPQTPVFPSTHDPRTVTLDFRNAGIPAPP PPLPPQPPPPPPPPPVEPTKLPFKELDNQWPSEAIPPGPRGRDEVTEEYMELAKSRGPWRRPPKKRHEDL VPPAGSPELSPPQPLFRPRSEFEEMTILYDIWNGGIDEEDIRFLCVTYERLLQQDNGMDWLNDTLWVYHP STSLSSAKKKKRDDGIREHVTGCARSEGFYTIDKKDKLRYLNSSRASTDEPPADTQGMSIPAQPHASTRA GSERRSEQRRLLSSFTGSCDSDLLKFNQLKFRKKKLKFCKSHIHDWGLFAMEPIAADEMVIEYVGQNIRQ VIADMREKRYEDEGIGSSYMFRVDHDTIIDATKCGNFARFINHSCNPNCYAKVITVESQKKIVIYSKQHI NVNEEITYDYKFPIEDVKIPCLCGSENCRGTLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015048
ORF Size	5769 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015048.1, NP_055863.1
RefSeq ORF	5772 bp
Locus ID	23067
UniProt ID	Q9UPS6
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation
MW	208.6 kDa
Gene Summary	SET1B is a component of a histone methyltransferase complex that produces trimethylated histone H3 at Lys4 (Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Mar 2008]

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