United States
Germany
Japan
United Kingdom
China
ARAP1 (NM_001135190) Human Tagged ORF Clone
CAT＃: RC226338
- TrueORF®
ARAP1 (Myc-DDK-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4
ORF Plasmid: tGFP
"NM_001135190" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 11,310.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CENTD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226338 representing NM_001135190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAGAAGGAGGAGCCCCCACCGAGCCGAGTCCCACGGGCCGTGCGCGTGGCCAGTCTGCTGAGCG AGGGAGAGGAACTGTCTGGGGACGACCAAGGGGATGAGGAAGAGGATGACCACGCCTATGAGGGCGTCCC CAATGGCGGATGGCATACCAGCAGCCTGAGCTTGTCCTTGCCCAGCACAATAGCTGCGCCACACCCCATG GACGGGCCGCCTGGGGGCTCCACCCCCGTCACACCAGTCATCAAGGCTGGCTGGCTGGACAAGAACCCAC CGCAGGGATCTTACATCTATCAGAAACGATGGGTGAGACTGGATACTGATCACCTGCGATACTTTGACAG TAACAAGGACGCTTACTCTAAGCGCTTTATCTCTGTGGCCTGCATCTCCCACGTGGCTGCCATCGGGGAC CAGAAGTTTGAAGTGATCACAAACAACCGAACCTTTGCCTTCCGGGCAGAGAGTGATGTGGAGCGGAAGG AGTGGATGCAGGCCCTGCAGCAGGCCATGGCTGAGCAGCGTGCCCGGGCCCGGCTCTCTAGCGCTTATCT GCTGGGAGTTCCAGGCTCAGAGCAGCCTGACCGCGCTGGCAGCCTGGAGCTTCGTGGCTTCAAGAATAAG CTGTACGTGGCCGTGGTCGGGGACAAAGTGCAGCTCTACAAGAATCTAGAGGAGTACCACCTGGGCATTG GCATCACCTTCATCGACATGAGCGTGGGCAACGTGAAGGAAGTGGACCGGCGCAGCTTCGACCTCACCAC GCCCTACCGCATCTTCAGCTTCTCTGCTGACTCAGAGCTAGAGAAGGAGCAGTGGTTGGAGGCCATGCAG GGAGCCATCGCTGAGGCCCTGTCTACCTCGGAGGTGGCCGAGCGCATCTGGGCTGCAGCCCCCAACAGGT TCTGTGCTGACTGCGGGGCTCCTCAGCCTGACTGGGCCTCCATCAACCTCTGTGTTGTTATCTGCAAGCG CTGTGCAGGGGAGCACCGTGGCCTGGGCGCTGGCGTCTCCAAGGTGCGGAGCCTGAAGATGGACAGGAAG GTGTGGACAGAAACACTTATCGAGGCCCTGTGTGCTGCAGTCACCACCACAGACCTGGCTGAGACCCAGG CGCTCCTGGGCTGTGGGGCTGGGATCAACTGCTTCTCGGGGGACCCTGAGGCCCCCACGCCCCTGGCTCT TGCAGAGCAGGCGGGGCAGACGCTGCAGATGGAATTCCTTCGGAACAACCGGACCACAGAGGTGCCTCGG CTGGACTCGATGAAGCCCCTGGAAAAGCACTACTCAGTTGTCCTGCCGACCGTGAGCCACAGTGGCTTCC TCTACAAGACTGCCTCTGCCGGCAAGCTGCTACAGGACCGCCGGGCCCGGGAAGAGTTCAGCCGGCGCTG GTGTGTCCTTGGTGACGGGGTCCTGAGCTACTTTGAGAATGAGCGGGCAGTGACCCCCAATGGAGAGATT CGGGCCAGCGAGATTGTGTGCCTGGCAGTGCCCCCTCCTGACACCCATGGCTTTGAGCACACCTTTGAGG TGTACACGGAGGGAGAACGGCTGTACCTGTTTGGGCTGGAGAGTGCGGAGCAGGCTCATGAGTGGGTCAA GTGTATTGCTAAGGCATTCGTGCCTCCCCTAGCCGAGGATCTGCTGGCCCGGGATTTTGAGCGGCTGGGA CGCCTACCCTACAAAGCTGGCCTGAGCCTACAGCGGGCCCAGGAGGGCTGGTTCTCTCTCAGTGGCTCGG AGCTCCGTGCTGTCTTCCCGGAGGGGCCCTGCGAAGAGCCGCTGCAACTACGGAAACTGCAGGAGCTTTC CATCCAGGGGGACAGTGAGAACCAGGTGCTGGTGCTGGTGGAGCGAAGGAGGACACTGTACATACAGGGC GAGCGGCGGCTGGACTTCATGGGTTGGCTGGGGGCCATCCAGAAAGCAGCCGCCAGCATGGGGGACACGC TGTCGGAGCAGCAGCTTGGGGACTCGGATATCCCGGTGATCGTGTACCGCTGTGTGGACTACATCACGCA GTGCGGCCTGACCTCCGAGGGCATCTACCGCAAGTGTGGGCAGACATCGAAGACACAGCGGCTGCTGGAG AGCCTGCGGCAGGATGCGCGCTCTGTGCACCTCAAGGAGGGCGAGCAGCACGTGGATGATGTTTCCTCGG CGCTCAAGCGCTTCCTGCGCGACCTGCCTGATGGGCTCTTCACTCGCGCCCAGCGCCTAACCTGGCTGGA GGCCTCAGAGATTGAGGACGAGGAGGAGAAGGTCTCCAGGTACCGAGAGCTGCTGGTGCGGCTGCCCCCT GTCAACCGGGCCACAGTGAAGGCCCTTATCAGCCACCTGTACTGTGTTCAGTGCTTCTCAGACACGAACC AGATGAACGTGCACAACCTGGCAATTGTGTTTGGGCCCACGCTCTTCCAGACAGATGGGCAGGACTACAA GGCTGGCCGTGTGGTGGAAGACCTCATTAACCACTATGTGGTGGTGTTTAGTGTGGATGAGGAAGAGCTC AGGAAGCAGCGGGAGGAGATCACTGCCATTGTGAAGATGCGCGTGGCTGGCACTGCCAGTGGGACCCAGC ATGCCGGTGACTTCATCTGCACAGTGTATCTGGAAGAGAAGAAGGCAGAGACTGAGCAGCATATCAAGGT CCCAGCATCCATGACTGCTGAGGAGCTCACCCTGGAGATCCTGGATCGCCGGAACGTGGGCATCAGGGAG AAGGACTATTGGACCTGCTTTGAGGTCAACGAGAGGGAGGAGGCAGAGCGCCCCCTGCACTTTGCGGAGA AGGTGCTGCCCATCCTGCACGGGCTGGGCACGGACAGCCACCTGGTGGTGAAGAAGCACCAGGCCATGGA GGCCATGCTGCTGTACCTGGCCAGCCGTGTCGGTGACACCAAGCATGGCATGATGAAGTTCCGTGAGGAC CGCAGCCTCCTGGGCCTGGGCCTGCCCTCAGGTGGCTTCCACGATCGCTACTTCATCCTCAACAGCAGCT GCTTGCGGCTCTACAAGGAGGTCCGGAGTCACCGGCCTGAGAAGGAGTGGCCTATTAAGAGTCTCAAAGT CTACCTGGGAGTGAAGAAGAAACTCAGGCCACCCACCTGCTGGGGCTTCACAGTGGTGCATGAGACAGAG AAACATGAGAAGCAGCAGTGGTACCTCTGCTGTGACACACAGATGGAGCTCCGGGAGTGGTTCGCTACCT TTCTGTTTGTGCAGCATGACGGCCTGGTGTGGCCCTCAGAGCCCTCACGCGTGTCCCGGGCAGTGCCTGA GGTCCGGCTGGGTAGTGTGTCACTGATCCCCCTTCGAGGTAGTGAAAATGAAATGCGCCGGAGTGTGGCT GCCTTCACCGCGGACCCTCTGTCTCTTCTGCGCAACGTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC226338 representing NM_001135190
Red=Cloning site Green=Tags(s) MTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPM DGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGD QKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNK LYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQ GAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRK VWTETLIEALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPR LDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEI RASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLG RLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQG ERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLE SLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPP VNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEEL RKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIRE KDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFRED RSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETE KHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVA AFTADPLSLLRNV SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135190 |
| ORF Size | 3399 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135190.1, NP_001128662.1 |
| RefSeq ORF | 3402 bp |
| Locus ID | 116985 |
| UniProt ID | Q96P48 |
| Protein Pathways | Endocytosis |
| MW | 127.5 kDa |
| Gene Summary | The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226338L3 | Lenti-ORF clone of ARAP1 (Myc-DDK-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4 |
CNY 13,210.00 |
|
| RC226338L4 | Lenti-ORF clone of ARAP1 (mGFP-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4 |
CNY 13,210.00 |
|
| RG226338 | ARAP1 (tGFP-tagged) - Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4 |
CNY 12,450.00 |
|
| SC325394 | ARAP1 (untagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4 |
CNY 10,450.00 |
