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IQSEC1 (NM_001134382) Human Tagged ORF Clone
CAT＃: RC226333
IQSEC1 (Myc-DDK-tagged)-Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1
ORF Plasmid: tGFP
"NM_001134382" in other vectors (6)
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CNY 7,760.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARF-GEP100; ARFGEP100; BRAG2; GEP100; IDDSSBA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226333 representing NM_001134382
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCAGAAGACGCTATTTCGTCGAGGGCGAGGCCCCCAGCAGTGAGACTGGCACATCCCTGGACA GCCCCTCAGCCTACCCCCAGGGCCCCTTGGTGCCCGGTTCCAGCCTGAGCCCGGATCACTACGAGCACAC GTCAGTGGGAGCCTATGGGCTGTACTCGGGGCCGCCGGGGCAACAGCAGCGCACGCGGAGGCCCAAGCTG CAGCACTCGACCTCCATCCTGCGCAAGCAGGCTGAGGAGGAGGCCATCAAGCGCTCACGCTCACTCTCCG AGAGCTATGAGCTCTCCTCGGACCTGCAGGACAAGCAGGTGGAGATGCTAGAACGAAAGTATGGGGGGCG CCTGGTAACCCGCCATGCGGCCCGCACCATCCAGACGGCGTTTCGCCAGTACCAGATGAACAAGAACTTC GAGCGCTTGCGCAGCTCCATGTCAGAGAACCGCATGTCACGCCGGATTGTGCTGTCCAACATGAGGATGC AGTTCTCCTTTGAGGGGCCTGAGAAAGTGCACAGCTCCTACTTCGAGGGGAAGCAGGTCTCAGTGACTAA CGACGGCTCCCAGCTGGGAGCCCTGGTGTCCCCTGAGTGTGGTGACCTCAGCGAGCCCACCACCCTCAAG TCTCCGGCCCCCTCCAGTGACTTTGCGGACGCCATCACCGAGCTGGAGGACGCCTTCTCTAGGCAAGTGA AATCACTGGCCGAGTCCATCGACGATGCCCTCAACTGCCGCAGCCTGCACACTGAGGAGGCACCGGCCCT GGATGCGGCGCGGGCCCGGGACACCGAACCCCAGACAGCCCTGCACGGCATGGACCACCGCAAACTGGAC GAGATGACGGCCTCGTACAGTGATGTCACCCTGTACATCGATGAGGAGGAGCTGTCGCCCCCTCTGCCCC TCTCGCAGGCAGGGGACCGGCCGTCCAGCACCGAGTCGGACCTGCGGCTACGGGCTGGGGGCGCAGCCCC AGACTACTGGGCCCTGGCCCACAAAGAGGACAAGGCTGACACGGACACGAGCTGCCGGAGCACGCCGTCG CTGGAGCGGCAGGAGCAGCGGCTGCGGGTGGAGCATCTGCCGCTGCTCACCATCGAGCCACCCAGCGACA GCTCTGTGGACCTTAGTGACCGCTCGGAGCGGGGGTCACTCAAGAGGCAGAGTGCTTACGAGCGCAGCCT TGGCGGGCAGCAGGGCAGTCCCAAGCATGGTCCCCACAGCGGCGCCCCCAAGAGCCTCCCCCGGGAGGAG CCTGAGTTGCGGCCCCGGCCCCCCAGGCCCCTGGACAGCCACTTGGCCATCAATGGCTCAGCCAACCGGC AGAGCAAGTCTGAGTCGGACTACTCAGACGGTGACAATGACAGCATCAACAGCACGTCCAACTCCAACGA TACCATCAACTGCAGCTCCGAGTCATCGTCCCGTGACAGCCTGCGGGAGCAGACGCTCAGCAAGCAGACC TACCACAAGGAGGCCCGCAACAGCTGGGACTCGCCTGCCTTTAGCAACGATGTCATCCGCAAGAGGCACT ACCGCATCGGCCTGAACCTCTTCAACAAGAAGCCTGAGAAGGGAGTCCAGTACCTCATCGAGCGTGGCTT TGTGCCCGACACGCCCGTCGGGGTGGCCCACTTCCTGCTGCAGCGCAAGGGCCTCAGCCGGCAGATGATC GGCGAGTTCCTGGGCAACCGGCAGAAGCAGTTCAACCGTGACGTGCTCGACTGCGTCGTGGACGAGATGG ACTTCTCTACCATGGAGCTGGATGAGGCCCTCAGGAAATTCCAGGCGCACATCCGTGTCCAAGGGGAGGC TCAGAAAGTGGAGCGGCTCATAGAGGCGTTCAGCCAGCGCTACTGCATCTGCAACCCTGGGGTGGTGCGG CAATTCCGGAACCCAGACACCATTTTCATCCTGGCCTTCGCCATCATCCTGCTGAACACCGACATGTACA GCCCCAATGTCAAGCCCGAGCGGAAAATGAAGCTAGAGGACTTCATCAAGAACCTCCGAGGTGTGGACGA TGGTGAGGACATTCCCCGTGAGATGCTGATGGGGATCTATGAACGGATCCGTAAGCGAGAGCTAAAGACC AATGAGGACCATGTGTCCCAGGTGCAGAAGGTGGAGAAGCTCATTGTGGGGAAAAAGCCGATCGGATCCC TGCATCCCGGGCTCGGCTGTGTGCTCTCTCTGCCCCACCGTCGGTTGGTCTGCTACTGCCGGCTCTTTGA GGTTCCAGACCCAAACAAGCCCCAGAAACTCGGACTACACCAGCGAGAAATCTTCCTGTTCAACGACCTC CTGGTGGTCACCAAGATCTTCCAGAAGAAGAAGAACTCGGTGACGTACAGCTTCCGACAGTCCTTCTCCT TGTACGGCATGCAGGTCCTGCTCTTCGAGAACCAGTACTACCCCAATGGCATCCGGCTCACCTCGTCTGT CCCCGGAGCAGATATCAAAGTGTTAATAAACTTCAACGCCCCCAACCCTCAAGACCGGAAGAAATTCACC GATGACCTGCGGGAGTCCATTGCGGAAGTCCAAGAGATGGAGAAGCACAGGATAGAGTCGGAGCTCGAGA AGCAGAAAGGCGTCGTGCGGCCCAGCATGTCCCAGTGCTCTAGCCTCAAAAAGGAGTCGGGCAACGGAAC ACTGAGCCGGGCCTGCCTGGACGACAGCTATGCCAGCGGTGAGGGCCTCAAGCGCAGCGCCCTCAGCAGC TCCCTGCGGGACCTCTCGGAAGCCGGGAAGCGAGGGCGTCGCAGCAGTGCGGGATCGCTAGAGAGCAATG TGGAAGGGTCCATCATTAGCAGTCCTCACATGCGCCGGAGAGCTACATCAACACGAGAGTGTCCATCTCG CCCACACCAGACTATGCCCAACTCATCTTCCCTCCTGGGCTCCTTATTCGGGAGCAAGAGAGGGAAGCCC CCTCCCCAGGCCCACCTGCCCTCAGCCCCAGCCCTGCCACCCCCCCACCCACCGGTGGTCCTGCCTCACT TGCAGCACTCTGTGGCTGGCCACCACCTGGGGCCCCCAGAGGGGCTGCCGCAGGCCGCCATGCACGGGCA TCACACCCAGTACTGCCACATGCAGAACCCTCCCCCGTACCACCATCACCACCACCACCACCCACCCCAG CACATCCAGCACGCACACCAGTACCACCACGGCCCCCATGGGGGCCACCCAGCCTACGGGGCCCATGCCC ACGGCCACCCGCCGCTGCCCTCGGCCCACGTGGGGCACACAGTGCACCACCATGGGCAGCCCCCTGCCCC GCCGCCCCCCACCAGCAGCAAGGCCAAACCCAGCGGCATCAGCACAATTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226333 representing NM_001134382
Red=Cloning site Green=Tags(s) MACRRRYFVEGEAPSSETGTSLDSPSAYPQGPLVPGSSLSPDHYEHTSVGAYGLYSGPPGQQQRTRRPKL QHSTSILRKQAEEEAIKRSRSLSESYELSSDLQDKQVEMLERKYGGRLVTRHAARTIQTAFRQYQMNKNF ERLRSSMSENRMSRRIVLSNMRMQFSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVSPECGDLSEPTTLK SPAPSSDFADAITELEDAFSRQVKSLAESIDDALNCRSLHTEEAPALDAARARDTEPQTALHGMDHRKLD EMTASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRAGGAAPDYWALAHKEDKADTDTSCRSTPS LERQEQRLRVEHLPLLTIEPPSDSSVDLSDRSERGSLKRQSAYERSLGGQQGSPKHGPHSGAPKSLPREE PELRPRPPRPLDSHLAINGSANRQSKSESDYSDGDNDSINSTSNSNDTINCSSESSSRDSLREQTLSKQT YHKEARNSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMI GEFLGNRQKQFNRDVLDCVVDEMDFSTMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVR QFRNPDTIFILAFAIILLNTDMYSPNVKPERKMKLEDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKT NEDHVSQVQKVEKLIVGKKPIGSLHPGLGCVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDL LVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSSVPGADIKVLINFNAPNPQDRKKFT DDLRESIAEVQEMEKHRIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYASGEGLKRSALSS SLRDLSEAGKRGRRSSAGSLESNVEGSIISSPHMRRRATSTRECPSRPHQTMPNSSSLLGSLFGSKRGKP PPQAHLPSAPALPPPHPPVVLPHLQHSVAGHHLGPPEGLPQAAMHGHHTQYCHMQNPPPYHHHHHHHPPQ HIQHAHQYHHGPHGGHPAYGAHAHGHPPLPSAHVGHTVHHHGQPPAPPPPTSSKAKPSGISTIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134382 |
| ORF Size | 3342 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134382.3 |
| RefSeq ORF | 3345 bp |
| Locus ID | 9922 |
| UniProt ID | Q6DN90 |
| Protein Pathways | Endocytosis |
| MW | 124.3 kDa |
| Gene Summary | Guanine nucleotide exchange factor for ARF1 and ARF6 (PubMed:24058294). Guanine nucleotide exchange factor activity is enhanced by lipid binding (PubMed:24058294). Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226333L1 | Lenti ORF clone of Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1, Myc-DDK-tagged |
CNY 10,160.00 |
|
| RC226333L2 | Lenti ORF clone of Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1, mGFP tagged |
CNY 13,680.00 |
|
| RC226333L3 | Lenti ORF clone of Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1, Myc-DDK-tagged |
CNY 13,680.00 |
|
| RC226333L4 | Lenti ORF clone of Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1, mGFP tagged |
CNY 13,680.00 |
|
| RG226333 | IQSEC1 (tGFP-tagged) - Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1 |
CNY 12,920.00 |
|
| SC326340 | IQSEC1 (untagged)-Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1 |
CNY 21,570.00 |
