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PDE4D (NM_001104631) Human Tagged ORF Clone
CAT＃: RC226179
- TrueORF®
PDE4D (Myc-DDK-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 1
ORF Plasmid: tGFP
"NM_001104631" in other vectors (4)
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CNY 9,480.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226179 representing NM_001104631
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGAGGGCAGCAGCGCGCCGGCCCGGGCGGGCAGCGGAGAGGGCAGCGACAGCGCCGGCGGGG CCACGCTCAAAGCCCCCAAGCATCTCTGGAGGCACGAGCAGCACCACCAGTACCCGCTCCGGCAGCCCCA GTTCCGCCTCCTGCATCCCCATCACCACCTGCCCCCGCCGCCGCCACCCTCGCCCCAGCCCCAGCCCCAG TGTCCGCTACAGCCGCCGCCGCCGCCCCCCCTGCCGCCGCCCCCGCCGCCGCCCGGGGCTGCCCGCGGCC GCTACGCCTCGAGCGGGGCCACCGGCCGCGTCCGGCATCGCGGCTACTCGGACACCGAGCGCTACCTGTA CTGTCGCGCCATGGACCGCACCTCCTACGCGGTGGAGACCGGCCACCGGCCCGGCCTGAAGAAATCCAGG ATGTCCTGGCCCTCCTCGTTCCAGGGACTCAGGCGTTTTGATGTGGACAATGGCACATCTGCGGGACGGA GTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTAATTCTCCAAGCAAATTTTGTCCACAGTCAACG ACGGGAGTCCTTCCTGTATCGATCCGACAGCGATTATGACCTCTCTCCAAAGTCTATGTCCCGGAACTCC TCCATTGCCAGTGATATACACGGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGTCTGC GAACTGTACGAAACAACTTTGCTGCATTAACTAATTTGCAAGATCGAGCACCTAGCAAAAGATCACCCAT GTGCAACCAACCATCCATCAACAAAGCCACCATAACAGAGGAGGCCTACCAGAAACTGGCCAGCGAGACC CTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTACAGACCAGGCACTCCGTCAGTGAGATGG CCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTCACCCATCTCTCTGAAATGAGTCGGTCTGGAAA TCAAGTGTCAGAGTTTATATCAAACACATTCTTAGATAAGCAACATGAAGTGGAAATTCCTTCTCCAACT CAGAAGGAAAAGGAGAAAAAGAAAAGACCAATGTCTCAGATCAGTGGAGTCAAGAAATTGATGCACAGCT CTAGTCTGACTAATTCAAGTATCCCAAGGTTTGGAGTTAAAACTGAACAAGAAGATGTCCTTGCCAAGGA ACTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGAATAGCAGAGTTGTCTGGTAACCGGCCCTTG ACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTATTAAAAACATTTAAAATTCCAGTAGATACTT TAATTACATATCTTATGACTCTCGAAGACCATTACCATGCTGATGTGGCCTATCACAACAATATCCATGC TGCAGATGTTGTCCAGTCTACTCATGTGCTATTATCTACACCTGCTTTGGAGGCTGTGTTTACAGATTTG GAGATTCTTGCAGCAATTTTTGCCAGTGCAATACATGATGTAGATCATCCTGGTGTGTCCAATCAATTTC TGATCAATACAAACTCTGAACTTGCCTTGATGTACAATGATTCCTCAGTCTTAGAGAACCATCATTTGGC TGTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATTTTCCAGAATTTGACCAAAAAACAAAGACAA TCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAGATATGTCAAAACACATGAATCTACTGGCTG ATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGCTCTGGAGTTCTTCTTCTTGATAATTATTCCGA TAGGATTCAGGTTCTTCAGAATATGGTGCACTGTGCAGATCTGAGCAACCCAACAAAGCCTCTCCAGCTG TACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGCCAAGGAGACCGAGAGAGGGAACGTGGCA TGGAGATAAGCCCCATGTGTGACAAGCACAATGCTTCCGTGGAAAAATCACAGGTGGGCTTCATAGACTA TATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTCCACCCTGACGCCCAGGATATTTTGGACACT TTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTCAGAGCCCCTCTCCTGCACCTGATGACCCAG AGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTTGAACTAACTTTAGAGGAAGATGGTGAGTCAGA CACGGAAAAGGACAGTGGCAGTCAAGTGGAAGAAGACACTAGCTGCAGTGACTCCAAGACTCTTTGTACT CAAGACTCAGAGTCTACTGAAATTCCCCTTGATGAACAGGTTGAAGAGGAGGCAGTAGGGGAAGAAGAGG AAAGCCAGCCTGAAGCCTGTGTCATAGATGATCGTTCTCCTGACACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226179 representing NM_001104631
Red=Cloning site Green=Tags(s) MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQPQ CPLQPPPPPPLPPPPPPPGAARGRYASSGATGRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSR MSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNS SIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASET LEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPT QKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPL TVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDL EILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQ SLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQL YRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDT LEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCT QDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001104631 |
| ORF Size | 2427 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001104631.2 |
| RefSeq ORF | 2430 bp |
| Locus ID | 5144 |
| UniProt ID | Q08499 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 90.9 kDa |
| Gene Summary | This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226179L3 | Lenti-ORF clone of PDE4D (Myc-DDK-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 1 |
CNY 14,060.00 |
|
| RC226179L4 | Lenti-ORF clone of PDE4D (mGFP-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 1 |
CNY 14,060.00 |
|
| RG226179 | PDE4D (tGFP-tagged) - Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 1 |
CNY 13,400.00 |
|
| SC317133 | PDE4D (untagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 1 |
CNY 15,580.00 |
