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PLCD1 (NM_001130964) Human Tagged ORF Clone
CAT＃: RC226161
- TrueORF®
PLCD1 (Myc-DDK-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 1
ORF Plasmid: tGFP
"NM_001130964" in other vectors (4)
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CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NDNC3; PLC-III |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226161 representing NM_001130964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGCCTGGGGATCCGGAGCCGGAGCCGCTCCAGGGAGCTCTACCTGCAGGAGCGGAGCCTTAAGG TGGCGGCGCTCAATGGACGGAGGCTGGGCCTACAGGATGATGAGGATCTACAGGCGCTGCTGAAGGGCAG CCAGCTCCTGAAGGTGAAGTCCAGCTCATGGAGGAGAGAGCGCTTCTACAAGTTGCAGGAGGACTGCAAG ACCATCTGGCAGGAGTCCCGCAAGGTCATGCGGACCCCGGAGTCCCAGCTGTTCTCCATCGAGGACATTC AGGAGGTGCGAATGGGGCACCGCACGGAGGGTCTGGAGAAGTTCGCCCGTGATGTGCCCGAGGACCGCTG CTTCTCCATTGTCTTCAAGGACCAGCGCAATACACTAGACCTCATCGCCCCATCGCCAGCTGATGCCCAG CACTGGGTGCTGGGGCTGCACAAGATCATCCACCACTCAGGCTCCATGGACCAGCGTCAGAAGCTACAGC ACTGGATTCACTCCTGCTTGCGAAAAGCTGACAAAAACAAGGACAACAAGATGAGCTTCAAGGAGCTGCA GAACTTCCTGAAGGAGCTCAACATCCAGGTGGACGACAGCTATGCCCGGAAGATCTTCAGGGAGTGTGAC CACTCCCAGACAGACTCCCTGGAGGACGAGGAGATTGAGGCCTTCTACAAGATGCTGACCCAGCGGGTGG AGATCGACCGCACCTTCGCCGAGGCCGCGGGCTCAGGGGAGACTCTGTCGGTGGATCAGTTAGTGACGTT CCTGCAGCACCAGCAGCGGGAGGAGGCGGCAGGGCCTGCGCTGGCCCTCTCCCTCATTGAGCGCTACGAG CCCAGCGAGACTGCCAAGGCGCAGCGGCAGATGACCAAGGACGGCTTCCTCATGTACTTACTGTCGGCTG ACGGCAGCGCCTTCAGCCTGGCACACCGCCGTGTCTACCAGGACATGGGCCAGCCACTTAGCCACTACCT GGTGTCCTCTTCACACAACACCTACCTGCTGGAGGACCAGCTAGCCGGGCCCAGCAGCACTGAAGCCTAC ATCCGGGCACTGTGCAAAGGCTGCCGATGCCTGGAGCTTGACTGCTGGGACGGGCCCAACCAGGAACCAA TCATCTACCACGGCTATACTTTCACTTCCAAGATCCTCTTCTGCGATGTGCTCAGGGCCATCCGGGACTA TGCCTTCAAGGCGTCCCCCTACCCTGTCATCCTATCCCTGGAGAACCACTGCACACTGGAGCAGCAGCGC GTGATGGCGCGGCACCTGCATGCCATCCTGGGCCCCATGCTGTTGAACCGACCACTGGATGGGGTCACCA ACAGCCTGCCCTCCCCTGAGCAACTGAAGGGGAAGATCCTGCTGAAGGGGAAGAAGCTCGGGGGGCTCCT GCCCCCTGGAGGGGAGGGTGGCCCTGAGGCCACTGTGGTGTCAGACGAAGACGAGGCTGCTGAGATGGAG GATGAGGCAGTGAGGAGCCGTGTGCAGCACAAGCCCAAGGAGGACAAGCTCAGGCTAGCACAGGAGCTCT CTGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGGGGCTTCTCCAGTCCTGGCACCCCTGGACAGGC CTTCTACGAGATGGCGTCCTTCTCTGAGAACCGTGCCCTTCGACTGCTCCAAGAATCAGGAAACGGCTTT GTCCGCCACAACGTGGGGCACCTGAGCAGAATCTACCCGGCTGGATGGAGAACAGACTCCTCCAACTACA GCCCCGTGGAGATGTGGAATGGGGGCTGCCAGATCGTGGCCCTGAATTTCCAGACACCTGGGCCAGAGAT GGACGTGTACCAGGGCCGCTTCCAGGACAACGGGGCCTGTGGGTACGTGCTGAAGCCCGCCTTCCTGCGA GACCCCAACGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCACGGAAGCGGCTCAACA TCAGGGTCATTTCGGGGCAGCAGCTGCCAAAAGTCAACAAGAATAAGAATTCAATTGTGGACCCCAAAGT GACAGTGGAGATCCATGGCGTGAGCCGGGACGTGGCCAGCCGCCAGACTGCTGTCATCACCAACAATGGT TTCAACCCATGGTGGGACACGGAGTTTGCGTTTGAGGTAGTTGTGCCTGACCTTGCCCTCATCCGCTTCT TGGTGGAAGATTATGATGCCTCCTCCAAGAATGACTTCATTGGCCAGAGTACCATCCCCTTGAACAGCCT CAAGCAAGGATACCGCCATGTCCACCTCATGTCTAAGAACGGGGACCAGCATCCATCAGCCACCCTCTTT GTGAAGATCTCCCTCCAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226161 representing NM_001130964
Red=Cloning site Green=Tags(s) MQCLGIRSRSRSRELYLQERSLKVAALNGRRLGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCK TIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQ HWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECD HSQTDSLEDEEIEAFYKMLTQRVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYE PSETAKAQRQMTKDGFLMYLLSADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAY IRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQR VMARHLHAILGPMLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEME DEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGF VRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLR DPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNG FNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLF VKISLQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130964 |
| ORF Size | 2331 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130964.2 |
| RefSeq ORF | 2334 bp |
| Locus ID | 5333 |
| UniProt ID | P51178 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 88 kDa |
| Gene Summary | This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226161L3 | Lenti-ORF clone of PLCD1 (Myc-DDK-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 1 |
CNY 10,070.00 |
|
| RC226161L4 | Lenti-ORF clone of PLCD1 (mGFP-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 1 |
CNY 10,070.00 |
|
| RG226161 | PLCD1 (tGFP-tagged) - Human phospholipase C, delta 1 (PLCD1), transcript variant 1 |
CNY 9,030.00 |
|
| SC326230 | PLCD1 (untagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 1 |
CNY 15,010.00 |
