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ECE1 (NM_001113347) Human Tagged ORF Clone
CAT＃: RC226144
- TrueORF®
ECE1 (Myc-DDK-tagged)-Human endothelin converting enzyme 1 (ECE1), transcript variant 3
ORF Plasmid: tGFP
"NM_001113347" in other vectors (4)
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CNY 8,080.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ECE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226144 representing NM_001113347
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTCCAGGGCCTGGGCCTGCAGCGGAACCCCTTCCTCCAAGGGAAGCGGGGCCCGGGGCTCACGT CTTCCCCGCCCCTCCTGCCTCCTTCCCTGCAGGTGAACTTCCACAGCCCCCGGAGTGGCCAGAGGTGCTG GGCTGCACGGACCCAGGTGGAGAAGCGGCTGGTGGTGTTGGTGGTACTTCTGGCGGCAGGACTGGTGGCC TGCTTGGCAGCACTGGGCATCCAGTACCAGACAAGATCCCCCTCTGTGTGCCTGAGCGAAGCTTGTGTCT CAGTGACCAGCTCCATCTTGAGCTCCATGGACCCCACAGTGGACCCCTGCCATGACTTCTTCAGCTACGC CTGTGGGGGCTGGATCAAGGCCAACCCAGTCCCTGATGGCCACTCACGCTGGGGGACCTTCAGCAACCTC TGGGAACACAACCAAGCAATCATCAAGCACCTCCTCGAAAACTCCACGGCCAGCGTGAGCGAGGCAGAGA GAAAGGCGCAAGTATACTACCGTGCGTGCATGAACGAGACCAGGATCGAGGAGCTCAGGGCCAAACCTCT AATGGAGTTGATTGAGAGGCTCGGGGGCTGGAACATCACAGGTCCCTGGGCCAAGGACAACTTCCAGGAC ACCCTGCAGGTGGTCACCGCCCACTACCGCACCTCACCCTTCTTCTCTGTCTATGTCAGTGCCGATTCCA AGAACTCCAACAGCAACGTGATCCAGGTGGACCAGTCTGGCCTGGGCTTGCCCTCGAGAGACTATTACCT GAACAAAACTGAAAACGAGAAGGTGCTGACCGGATATCTGAACTACATGGTCCAGCTGGGGAAGCTGCTG GGCGGCGGGGACGAGGAGGCCATCCGGCCCCAGATGCAGCAGATCTTGGACTTTGAGACGGCACTGGCCA ACATCACCATCCCACAGGAGAAGCGCCGTGATGAGGAGCTCATCTACCACAAAGTGACGGCAGCCGAGCT GCAGACCTTGGCACCCGCCATCAACTGGTTGCCTTTTCTCAACACCATCTTCTACCCCGTGGAGATCAAT GAATCCGAGCCTATTGTGGTCTATGACAAGGAATACCTTGAGCAGATCTCCACTCTCATCAACACCACCG ACAGATGCCTGCTCAACAACTACATGATCTGGAACCTGGTGCGGAAAACAAGCTCCTTCCTTGACCAGCG CTTTCAGGACGCCGATGAGAAGTTCATGGAAGTCATGTACGGGACCAAGAAGACCTGTCTTCCTCGCTGG AAGTTTTGCGTGAGTGACACAGAAAACAACCTGGGCTTTGCGTTGGGCCCCATGTTTGTCAAAGCAACCT TCGCCGAGGACAGCAAGAGCATAGCCACCGAGATCATCCTGGAGATTAAGAAGGCATTTGAGGAAAGCCT GAGCACCCTGAAGTGGATGGATGAGGAAACCCGAAAATCAGCCAAGGAAAAGGCCGATGCCATCTACAAC ATGATAGGATACCCCAACTTCATCATGGATCCCAAGGAGCTGGACAAAGTGTTTAATGACTACACTGCAG TTCCAGACCTCTACTTTGAAAATGCCATGCGGTTTTTCAACTTCTCATGGAGGGTCACTGCCGATCAGCT CAGGAAAGCCCCCAACAGAGATCAGTGGAGCATGACCCCGCCCATGGTGAACGCCTACTACTCGCCCACC AAGAATGAGATTGTGTTTCCGGCCGGGATCCTGCAGGCACCATTCTACACACGCTCCTCACCCAAGGCCT TAAACTTTGGTGGCATAGGTGTCGTCGTGGGCCATGAGCTGACTCATGCTTTTGATGATCAAGGACGGGA GTATGACAAGGACGGGAACCTCCGGCCATGGTGGAAGAACTCATCCGTGGAGGCCTTCAAGCGTCAGACC GAGTGCATGGTAGAGCAGTACAGCAACTACAGCGTGAACGGGGAGCCGGTGAACGGGCGGCACACCCTGG GGGAGAACATCGCCGACAACGGGGGTCTCAAGGCGGCCTATCGGGCTTACCAGAACTGGGTGAAGAAGAA CGGGGCTGAGCACTCGCTCCCCACCCTGGGCCTCACCAATAACCAGCTCTTCTTCCTGGGCTTTGCACAG GTCTGGTGCTCCGTCCGCACACCTGAGAGCTCCCACGAAGGCCTCATCACCGATCCCCACAGCCCCTCTC GCTTCCGGGTCATCGGCTCCCTCTCCAATTCCAAGGAGTTCTCAGAACACTTCCGCTGCCCACCTGGCTC ACCCATGAACCCGCCTCACAAGTGCGAAGTCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226144 representing NM_001113347
Red=Cloning site Green=Tags(s) MPLQGLGLQRNPFLQGKRGPGLTSSPPLLPPSLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVA CLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNL WEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQD TLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLL GGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEIN ESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRW KFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYN MIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPT KNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQT ECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQ VWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113347 |
| ORF Size | 2274 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113347.2 |
| RefSeq ORF | 2277 bp |
| Locus ID | 1889 |
| UniProt ID | P42892 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 85.6 kDa |
| Gene Summary | The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226144L3 | Lenti-ORF clone of ECE1 (Myc-DDK-tagged)-Human endothelin converting enzyme 1 (ECE1), transcript variant 3 |
CNY 9,980.00 |
|
| RC226144L4 | Lenti-ORF clone of ECE1 (mGFP-tagged)-Human endothelin converting enzyme 1 (ECE1), transcript variant 3 |
CNY 9,980.00 |
|
| RG226144 | ECE1 (tGFP-tagged) - Human endothelin converting enzyme 1 (ECE1), transcript variant 3 |
CNY 8,930.00 |
|
| SC318977 | ECE1 (untagged)-Human endothelin converting enzyme 1 (ECE1), transcript variant 3 |
CNY 14,730.00 |
