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CRY2 (NM_001127457) Human Tagged ORF Clone
CAT＃: RC225913
- TrueORF®
CRY2 (Myc-DDK-tagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 2
ORF Plasmid: tGFP
"NM_001127457" in other vectors (4)
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CNY 3,960.00
CNY 4,660.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HCRY2; PHLL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225913 representing NM_001127457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTCCACCCGGGCGGACCCACACATGGTTCCTACTTCAGTCTCTGGAAGATTTGGACACAAGTT TAAGGAAACTGAACTCCCGCCTGTTTGTAGTCCGGGGACAGCCAGCCGACGTGTTCCCAAGGCTGTTCAA GGAATGGGGAGTGACCCGCTTGACCTTTGAATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCC ATCATGAAGATGGCCAAGGAGGCTGGTGTGGAAGTAGTGACGGAGAATTCTCATACCCTCTATGACCTGG ACAGGATCATTGAGCTGAATGGGCAGAAGCCACCCCTTACATACAAGCGCTTTCAGGCCATCATCAGCCG CATGGAGCTGCCCAAGAAGCCAGTGGGCTTGGTGACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATC CAGGAGAACCACGACGAGACCTACGGCGTGCCCTCCCTGGAGGAGCTGGGGTTCCCCACTGAAGGACTTG GTCCAGCTGTCTGGCAGGGAGGAGAGACAGAAGCTCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGC CTGGGTTGCCAACTATGAGAGACCCCGAATGAACGCCAACTCCCTCCTGGCCAGCCCCACAGGCCTCAGC CCCTACCTGCGCTTTGGTTGTCTCTCCTGCCGCCTCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGG TGAAGCGGAACAGCACACCTCCCCTCTCCCTATTTGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGC AGCTACCAACAACCCCAGGTTTGACCGCATGGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGC AATCCTGAGGCCCTGGCCAAGTGGGCTGAGGGCAAGACAGGCTTCCCTTGGATTGATGCCATCATGACCC AACTGAGGCAGGAGGGCTGGATCCACCACCTGGCCCGGCATGCCGTGGCCTGCTTCCTGACCCGCGGGGA CCTCTGGGTCAGCTGGGAGAGCGGGGTCCGGGTATTTGATGAGCTGCTCCTGGATGCAGATTTCAGCGTG AACGCAGGCAGCTGGATGTGGCTGTCCTGCAGTGCTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTG TGGGCTTTGGCCGTCGCACGGACCCCAGTGGGGACTACATCAGGCGATACCTGCCCAAATTGAAAGCGTT CCCCTCTCGATACATCTATGAGCCCTGGAATGCCCCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATT GGTGTGGACTACCCACGGCCCATCGTCAACCATGCCGAGACCAGCCGGCTTAACATTGAACGAATGAAGC AGATTTACCAGCAGCTTTCGCGCTACCGGGGACTCTGTCTACTGGCATCTGTCCCTTCCTGTGTGGAAGA CCTCAGTCACCCTGTGGCAGAGCCCAGCTCGAGCCAGGCTGGCAGCATGAGCAGTGCAGGCCCAAGACCA CTACCCAGTGGCCCAGCATCCCCCAAACGCAAGCTGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCA GCAAACGGGCCCGGGTGGCAGAGTTGCCAACCCCAGAGCTGCCGAGCAAGGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225913 representing NM_001127457
Red=Cloning site Green=Tags(s) MPAPPGRTHTWFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAA IMKMAKEAGVEVVTENSHTLYDLDRIIELNGQKPPLTYKRFQAIISRMELPKKPVGLVTSQQMESCRAEI QENHDETYGVPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLS PYLRFGCLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDR NPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSV NAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKAFPSRYIYEPWNAPESIQKAAKCII GVDYPRPIVNHAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPSSSQAGSMSSAGPRP LPSGPASPKRKLEAAEEPPGEELSKRARVAELPTPELPSKDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001127457 |
| ORF Size | 1596 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001127457.2 |
| RefSeq ORF | 1599 bp |
| Locus ID | 1408 |
| UniProt ID | Q49AN0 |
| Protein Families | Druggable Genome |
| Protein Pathways | Circadian rhythm - mammal |
| MW | 60.4 kDa |
| Gene Summary | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225913L3 | Lenti-ORF clone of CRY2 (Myc-DDK-tagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 2 |
CNY 6,560.00 |
|
| RC225913L4 | Lenti-ORF clone of CRY2 (mGFP-tagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 2 |
CNY 6,360.00 |
|
| RG225913 | CRY2 (tGFP-tagged) - Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 2 |
CNY 5,130.00 |
|
| SC323124 | CRY2 (untagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 2 |
CNY 8,550.00 |
