AP1M1 (NM_001130524) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AP47; CLAPM2; CLTNM; MU-1A; mu1A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC225713 representing NM_001130524 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGCCAGCGCCGTCTACGTGCTGGACCTGAAGGGCAAGGTGCTCATCTGCCGGAACTACCGTGGCG ACGTGGACATGTCAGAGGTGGAGCACTTCATGCCCATCCTGATGGAGAAGGAGGAGGAGGGGATGCTGTC GCCCATCCTGGCCCACGGGGGGGTCCGTTTCATGTGGATCAAACACAACAACCTGTATCTGGTTGCCACA TCCAAGAAGAACGCGTGCGTGTCGCTGGTCTTTTCTTTCCTCTATAAGGTGGTGCAGGTGTTTTCCGAGT ACTTCAAGGAGCTGGAGGAGGAGAGCATCCGGGACAACTTTGTTATCATCTACGAGCTGCTGGACGAGCT CATGGACTTCGGCTACCCCCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGGAAGGCCAC AAGCTGGAAACAGGGGCCCCGCGGCCACCAGCCACCGTCACCAACGCGGTGTCCTGGCGGTCCGAAGGCA TCAAGTATCGGAAGAATGAGGTGTTCTTGGACGTCATCGAGTCTGTCAACCTCTTGGGTAAATACCCAGG AGTGGGATGGCTGGGTCACACGGTCAGCGCCAACGGCAATGTCCTGCGCAGCGAGATCGTGGGCTCCATC AAGATGCGAGTCTTCCTCTCGGGCATGCCCGAGCTGCGCCTGGGCCTCAACGACAAGGTCCTCTTTGACA ACACGGGCCGCGGCAAAAGCAAATCCGTGGAGCTGGAGGATGTGAAGTTCCACCAGTGTGTGCGGCTATC ACGCTTCGAGAATGACCGCACCATCTCCTTCATCCCACCCGACGGCGAGTTCGAGCTCATGTCCTACCGT CTCAACACCCACGTCAAGCCTTTGATATGGATCGAGTCGGTGATCGAGAAGCACTCCCACAGCCGCATCG AGTACATGATCAAGGCCAAAAGCCAGTTCAAGCGGCGGTCAACAGCCAACAACGTGGAGATCCACATTCC CGTGCCCAATGATGCCGACTCACCCAAGTTCAAGACGACGGTGGGGAGCGTTAAGTGGGTCCCCGAGAAC AGCGAGATCGTGTGGTCCATCAAGTCCTTCCCGGGCGGCAAGGAGTACCTGATGCGGGCCCACTTCGGCC TGCCTAGTGTGGAGGCCGAAGACAAGGAGGGCAAGCCCCCGATCAGTGTCAAGTTCGAGATCCCTTACTT CACTACCTCCGGCATCCAGGTGCGCTACCTGAAGATCATTGAGAAGAGTGGGTACCAGGCCCTGCCCTGG GTGCGTTATATCACGCAGAATGGAGATTACCAGCTCCGGACCCAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC225713 representing NM_001130524 Red=Cloning site Green=Tags(s) MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVAT SKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGH KLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVNLLGKYPGVGWLGHTVSANGNVLRSEIVGSI KMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYR LNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPEN SEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPW VRYITQNGDYQLRTQ TRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130524
ORF Size	1305 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130524.2
RefSeq ORF	1308 bp
Locus ID	8907
UniProt ID	Q9BXS5
Protein Pathways	Lysosome
MW	49.7 kDa
Gene Summary	The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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