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Cyclin A1 (CCNA1) (NM_001111046) Human Tagged ORF Clone
CAT＃: RC225673
- TrueORF®
CCNA1 (Myc-DDK-tagged)-Human cyclin A1 (CCNA1), transcript variant 3
ORF Plasmid: tGFP
"NM_001111046" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT146 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225673 representing NM_001111046
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCGGCTTTCCCGCAATCATGTACCCTGGATCTTTTATTGGGGGCTGGGGAGAAGAGTATCTCA GCTGGGAAGGACCGGGGCTCCCAGATTTCGTCTTCCAGCAGCCCGTGGAGTCTGAAGCAATGCACTGCAG CAACCCCAAGAGTGGAGTTGTGCTGGCTACAGTGGCCCGAGGTCCCGATGCTTGTCAGATACTCACCAGA GCCCCGCTGGGCCAGGATCCCCCGCAGAGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGA GGACCTGTGGCCAGGGGATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGG AAAGAAAGCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGATGAA CTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGATGTGTATGAAGTAG ACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAACACAGTTTCCCCTATGCTGGTAGA TTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGTCTTGGCACAGATGTGATAAATGTGACTGAATAT GCTGAAGAAATTTATCAGTACCTTAGGGAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGA AGCAGCCAGACATCACGGAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATA TAAACTTCGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCTGTT CTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATATGAAGAGATATATC CTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACAAAACGACAACTGTTAAAAATGGA ACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACAGTACCAACCACCAACCAGTTTCTCCTTCAGTAC TTGAGGCGACAAGGAGTGTGCGTCAGGACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTG AAGCAGATCCATTCTTGAAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATAC TGTGAACAAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTGCCT TGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCAATTAGGGAGAAGT ACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCAGTTCTTCTTCTACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225673 representing NM_001111046
Red=Cloning site Green=Tags(s) METGFPAIMYPGSFIGGWGEEYLSWEGPGLPDFVFQQPVESEAMHCSNPKSGVVLATVARGPDACQILTR APLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRCYSGSENAFPPAGKKALPDCGVQEPPKQGFDIYMDE LEQGDRDSCSVREGMAFEDVYEVDTGTLKSDLHFLLDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEY AEEIYQYLREAEIRHRPKAHYMKKQPDITEGMRTILVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSV LRGKLQLVGTAAMLLASKYEEIYPPEVDEFVYITDDTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQY LRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSLIAAAAFCLANYTVNKHFWPETLAAFTGYSLSEIVP CLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLMEPPAVLLLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111046 |
| ORF Size | 1395 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111046.1, NP_001104516.1 |
| RefSeq Size | 1844 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 8900 |
| UniProt ID | P78396 |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Cell cycle, Pathways in cancer, Progesterone-mediated oocyte maturation |
| MW | 52.2 kDa |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225673L3 | Lenti-ORF clone of CCNA1 (Myc-DDK-tagged)-Human cyclin A1 (CCNA1), transcript variant 3 |
CNY 5,890.00 |
|
| RC225673L4 | Lenti-ORF clone of CCNA1 (mGFP-tagged)-Human cyclin A1 (CCNA1), transcript variant 3 |
CNY 5,890.00 |
|
| RG225673 | CCNA1 (tGFP-tagged) - Human cyclin A1 (CCNA1), transcript variant 3 |
CNY 4,370.00 |
|
| SC317198 | CCNA1 (untagged)-Human cyclin A1 (CCNA1), transcript variant 3 |
CNY 7,220.00 |
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