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Legumain (LGMN) (NM_005606) Human Tagged ORF Clone
CAT＃: RC224975
LGMN (Myc-DDK-tagged)-Human legumain (LGMN), transcript variant 1
ORF Plasmid: tGFP
"NM_005606" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AEP; LGMN1; PRSC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224975 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTGGAAAGTAGCTGTATTCCTCAGTGTGGCCCTGGGCATTGGTGCCATTCCTATAGATGATCCTG AAGATGGAGGCAAGCACTGGGTGGTGATCGTGGCAGGTTCAAATGGCTGGTATAATTATAGGCACCAGGC AGACGCGTGCCATGCCTACCAGATCATTCACCGCAATGGGATTCCTGACGAACAGATCGTTGTGATGATG TACGATGACATTGCTTACTCTGAAGACAATCCCACTCCAGGAATTGTGATCAACAGGCCCAATGGCACAG ATGTCTATCAGGGAGTCCCGAAGGACTACACTGGAGAGGATGTTACCCCACAAAATTTCCTTGCTGTGTT GAGAGGCGATGCAGAAGCAGTGAAGGGCATAGGATCCGGCAAAGTCCTGAAGAGTGGCCCCCAGGATCAC GTGTTCATTTACTTCACTGACCATGGATCTACTGGAATACTGGTTTTTCCCAATGAAGATCTTCATGTAA AGGACCTGAATGAGACCATCCATTACATGTACAAACACAAAATGTACCGAAAGATGGTGTTCTACATTGA AGCCTGTGAGTCTGGGTCCATGATGAACCACCTGCCGGATAACATCAATGTTTATGCAACTACTGCTGCC AACCCCAGAGAGTCGTCCTACGCCTGTTACTATGATGAGAAGAGGTCCACGTACCTGGGGGACTGGTACA GCGTCAACTGGATGGAAGACTCGGACGTGGAAGATCTGACTAAAGAGACCCTGCACAAGCAGTACCACCT GGTAAAATCGCACACCAACACCAGCCACGTCATGCAGTATGGAAACAAAACAATCTCCACCATGAAAGTG ATGCAGTTTCAGGGTATGAAACGCAAAGCCAGTTCTCCCGTCCCCCTACCTCCAGTCACACACCTTGACC TCACCCCCAGCCCTGATGTGCCTCTCACCATCATGAAAAGGAAACTGATGAACACCAATGATCTGGAGGA GTCCAGGCAGCTCACGGAGGAGATCCAGCGGCATCTGGATGCCAGGCACCTCATTGAGAAGTCAGTGCGT AAGATCGTCTCCTTGCTGGCAGCGTCCGAGGCTGAGGTGGAGCAGCTCCTGTCCGAGAGAGCCCCGCTCA CGGGGCACAGCTGCTACCCAGAGGCCCTGCTGCACTTCCGGACCCACTGCTTCAACTGGCACTCCCCCAC GTACGAGTATGCGTTGAGACATTTGTACGTGCTGGTCAACCTTTGTGAGAAGCCGTATCCACTTCACAGG ATAAAATTGTCCATGGACCACGTGTGCCTTGGTCACTAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC224975 protein sequence
Red=Cloning site Green=Tags(s) MVWKVAVFLSVALGIGAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMM YDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDH VFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAA NPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKV MQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVR KIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHR IKLSMDHVCLGHY SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005606 |
| ORF Size | 1299 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005606.4 |
| RefSeq Size | 2073 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 5641 |
| UniProt ID | Q99538 |
| Domains | Peptidase_C13 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Antigen processing and presentation, Lysosome |
| MW | 49.4 kDa |
| Gene Summary | This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Processing of progranulin into granulins involves multiple lysosomal proteases and is affected in frontotemporal lobar degeneration
,Mohan, S;Sampognaro, P;Argouarch, A;Maynard, J;Patwardhan, A;Courtney, E;Mason, A;Li, K;Seeley, W;Miller, B;Burlingame, A;Jacobson, M;Kao, A;,
Research Square
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224975L1 | Lenti ORF clone of Human legumain (LGMN), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224975L2 | Lenti ORF clone of Human legumain (LGMN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC224975L3 | Lenti ORF clone of Human legumain (LGMN), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224975L4 | Lenti ORF clone of Human legumain (LGMN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG224975 | LGMN (tGFP-tagged) - Human legumain (LGMN), transcript variant 1 |
CNY 4,370.00 |
|
| SC116627 | LGMN (untagged)-Human legumain (LGMN), transcript variant 1 |
CNY 2,950.00 |
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