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AASS (NM_005763) Human Tagged ORF Clone
CAT＃: RC224831
- TrueORF®
AASS (Myc-DDK-tagged)-Human aminoadipate-semialdehyde synthase (AASS), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_005763" in other vectors (4)
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CNY 10,168.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LKR/SDH; LKRSDH; LORSDH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224831 representing NM_005763
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAAGTACATAGGACTGGACTGGGCAGGCTGGGGGTCAGCCTCTCCAAGGGTCTTCACCACAAAG CTGTGTTGGCCGTCCGGAGGGAGGATGTGAACGCCTGGGAGAGAAGGGCCCCGCTAGCTCCCAAGCACAT CAAAGGCATCACCAATCTGGGATACAAGGTCTTGATACAGCCTTCGAATCGGCGGGCCATTCATGATAAG GACTATGTCAAAGCTGGTGGCATTCTTCAGGAGGATATTTCTGAAGCTTGTCTAATTTTAGGAGTTAAAA GACCTCCAGAGGAAAAATTAATGTCCAGGAAGACTTATGCATTTTTCTCCCACACAATAAAAGCTCAGGA GGCCAATATGGGCTTGTTGGATGAGATTCTAAAACAGGAAATTCGCCTTATTGATTATGAGAAAATGGTG GATCATAGAGGAGTACGGGTAGTGGCATTTGGACAGTGGGCTGGTGTGGCAGGAATGATCAACATTTTAC ATGGAATGGGTTTAAGGCTCCTTGCTTTGGGACATCACACACCTTTTATGCACATTGGCATGGCTCATAA CTACAGGAATAGCAGTCAGGCTGTGCAAGCTGTCCGTGATGCTGGCTATGAAATATCTTTGGGTTTGATG CCTAAGTCAATAGGACCCTTAACATTTGTGTTCACAGGAACTGGTAATGTTTCTAAGGGAGCCCAAGCAA TCTTTAATGAGCTACCTTGTGAATATGTGGAGCCCCATGAATTAAAAGAAGTTTCCCAAACTGGAGACCT CAGAAAAGTGTATGGGACGGTGTTAAGTCGTCATCATCATCTTGTCAGGAAAACAGATGCTGTGTATGAT CCTGCAGAGTATGACAAACATCCGGAGCGCTACATAAGTCGTTTTAATACTGATATTGCACCCTATACAA CTTGCTTAATTAATGGAATCTACTGGGAACAAAACACTCCTCGCCTCCTAACCCGCCAAGATGCTCAGAG TCTCCTGGCTCCGGGCAAGTTCTCACCTGCTGGTGTGGAAGGCTGCCCTGCATTACCACACAAACTCGTG GCAATATGTGACATTTCAGCTGACACAGGAGGGTCTATAGAGTTTATGACTGAGTGTACAACAATAGAGC ATCCCTTTTGCATGTATGATGCAGACCAGCATATTATTCATGACAGTGTTGAAGGCTCGGGGATCCTGAT GTGTTCCATTGACAATTTGCCGGCACAGCTCCCAATTGAAGCTACAGAATGCTTTGGAGACATGCTTTAC CCTTATGTTGAAGAAATGATATTATCAGACGCGACACAGCCTCTTGAAAGTCAGAATTTTTCTCCTGTGG TGAGAGATGCAGTGATTACATCCAACGGTACATTACCTGATAAATATAAATATATCCAGACACTCCGGGA GAGCAGGGAACGTGCTCAGTCACTTTCAATGGGCACCAGGAGAAAGGTTTTGGTTCTTGGATCTGGCTAC ATATCTGAGCCTGTATTAGAATATTTATCAAGAGATGGCAATATAGAAATAACAGTAGGATCTGACATGA AGAATCAAATTGAACAGTTAGGCAAGAAATATAATATTAATCCTGTTAGCATGGACATTTGTAAACAAGA AGAGAAGCTGGGCTTCTTGGTGGCAAAACAGGATCTTGTCATCAGCTTGTTGCCTTATGTATTGCACCCT CTTGTGGCCAAGGCCTGCATCACAAACAAAGTTAACATGGTCACTGCAAGCTACATCACACCAGCACTAA AAGAATTGGAAAAGAGTGTGGAAGATGCTGGCATCACAATCATTGGTGAATTGGGATTGGACCCTGGTCT GGATCACATGTTAGCAATGGAAACAATAGATAAAGCCAAGGAAGTGGGAGCCACGATTGAATCATATATT TCCTACTGTGGTGGGCTTCCAGCCCCTGAACATTCAAACAATCCATTGAGATATAAATTTAGCTGGAGTC CAGTGGGAGTTTTGATGAATGTAATGCAGTCTGCCACCTATCTGCTCGATGGAAAGGTTGTGAATGTTGC AGGAGGCATCTCCTTTCTTGATGCCGTTACGTCCATGGATTTTTTTCCAGGATTAAATTTGGAAGGCTAT CCTAACAGAGACAGTACGAAATATGCTGAGATTTATGGCATTTCTTCTGCTCACACTTTGTTGCGGGGGA CACTGAGATATAAGGGATATATGAAAGCTTTGAATGGATTTGTAAAATTAGGTCTTATAAACAGAGAAGC GCTTCCTGCCTTTAGACCTGAGGCCAACCCTCTCACCTGGAAACAACTCCTCTGTGACCTAGTTGGGATT TCACCCTCCTCTGAGCATGATGTGTTGAAGGAAGCTGTTCTTAAGAAACTAGGAGGAGACAATACCCAGT TGGAGGCTGCTGAATGGTTGGGCTTACTTGGGGATGAACAAGTTCCTCAGGCAGAGTCCATTCTGGATGC CCTCTCCAAGCATTTGGTCATGAAGCTTTCCTATGGTCCTGAAGAAAAAGATATGATTGTGATGAGAGAC AGCTTTGGAATCAGACATCCTTCTGGACATTTAGAACATAAAACGATTGATCTTGTGGCTTATGGGGACA TCAATGGCTTTTCAGCCATGGCTAAAACCGTGGGGTTACCCACCGCCATGGCAGCCAAAATGTTGCTTGA TGGTGAAATTGGAGCCAAAGGCCTAATGGGGCCCTTTTCAAAGGAGATCTATGGACCAATATTGGAGCGA ATTAAAGCAGAAGGCATTATATATACTACACAGAGTACAATTAAACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224831 representing NM_005763
Red=Cloning site Green=Tags(s) MLQVHRTGLGRLGVSLSKGLHHKAVLAVRREDVNAWERRAPLAPKHIKGITNLGYKVLIQPSNRRAIHDK DYVKAGGILQEDISEACLILGVKRPPEEKLMSRKTYAFFSHTIKAQEANMGLLDEILKQEIRLIDYEKMV DHRGVRVVAFGQWAGVAGMINILHGMGLRLLALGHHTPFMHIGMAHNYRNSSQAVQAVRDAGYEISLGLM PKSIGPLTFVFTGTGNVSKGAQAIFNELPCEYVEPHELKEVSQTGDLRKVYGTVLSRHHHLVRKTDAVYD PAEYDKHPERYISRFNTDIAPYTTCLINGIYWEQNTPRLLTRQDAQSLLAPGKFSPAGVEGCPALPHKLV AICDISADTGGSIEFMTECTTIEHPFCMYDADQHIIHDSVEGSGILMCSIDNLPAQLPIEATECFGDMLY PYVEEMILSDATQPLESQNFSPVVRDAVITSNGTLPDKYKYIQTLRESRERAQSLSMGTRRKVLVLGSGY ISEPVLEYLSRDGNIEITVGSDMKNQIEQLGKKYNINPVSMDICKQEEKLGFLVAKQDLVISLLPYVLHP LVAKACITNKVNMVTASYITPALKELEKSVEDAGITIIGELGLDPGLDHMLAMETIDKAKEVGATIESYI SYCGGLPAPEHSNNPLRYKFSWSPVGVLMNVMQSATYLLDGKVVNVAGGISFLDAVTSMDFFPGLNLEGY PNRDSTKYAEIYGISSAHTLLRGTLRYKGYMKALNGFVKLGLINREALPAFRPEANPLTWKQLLCDLVGI SPSSEHDVLKEAVLKKLGGDNTQLEAAEWLGLLGDEQVPQAESILDALSKHLVMKLSYGPEEKDMIVMRD SFGIRHPSGHLEHKTIDLVAYGDINGFSAMAKTVGLPTAMAAKMLLDGEIGAKGLMGPFSKEIYGPILER IKAEGIIYTTQSTIKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005763 |
| ORF Size | 2778 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005763.4 |
| RefSeq Size | 3233 bp |
| RefSeq ORF | 2781 bp |
| Locus ID | 10157 |
| UniProt ID | Q9UDR5 |
| Domains | Saccharop_dh, AlaDh_PNT_C, AlaDh_PNT_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine biosynthesis, Lysine degradation, Metabolic pathways |
| MW | 102 kDa |
| Gene Summary | This gene encodes a bifunctional enzyme that catalyzes the first two steps in the mammalian lysine degradation pathway. The N-terminal and the C-terminal portions of this enzyme contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively, resulting in the conversion of lysine to alpha-aminoadipic semialdehyde. Mutations in this gene are associated with familial hyperlysinemia. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224831L3 | Lenti ORF clone of Human aminoadipate-semialdehyde synthase (AASS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC224831L4 | Lenti ORF clone of Human aminoadipate-semialdehyde synthase (AASS), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 11,690.00 |
|
| RG224831 | AASS (tGFP-tagged) - Human aminoadipate-semialdehyde synthase (AASS), nuclear gene encoding mitochondrial protein |
CNY 10,740.00 |
|
| SC314936 | AASS (untagged)-Human aminoadipate-semialdehyde synthase (AASS), nuclear gene encoding mitochondrial protein |
CNY 17,860.00 |
