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nkx6.1 (NKX6-1) (NM_006168) Human Tagged ORF Clone
CAT＃: RC224762
NKX6 (Myc-DDK-tagged)-Human NK6 homeobox 1 (NKX6-1)
ORF Plasmid: tGFP
"NM_006168" in other vectors (6)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NKX6.1; NKX6A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224762 representing NM_006168
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGCGGTGGGGGCAATGGAGGGCACCCGGCAGAGCGCATTCCTGCTCAGCAGCCCTCCCCTGGCCG CCCTGCACAGCATGGCCGAGATGAAGACCCCGCTGTACCCTGCCGCGTATCCCCCGCTGCCTGCCGGCCC CCCCTCCTCCTCGTCCTCGTCGTCGTCCTCCTCGTCGCCCTCCCCGCCTCTGGGCACCCACAACCCAGGC GGCCTGAAGCCCCCGGCCACGGGGGGGCTCTCATCCCTCGGCAGCCCCCCGCAGCAGCTCTCGGCCGCCA CCCCACACGGCATCAACGATATCCTGAGCCGGCCCTCCATGCCCGTGGCCTCGGGGGCCGCCCTGCCCTC CGCCTCGCCCTCCGGTTCCTCCTCCTCCTCTTCCTCGTCCGCCTCTGCCTCCTCCGCCTCTGCCGCCGCC GCGGCTGCTGCCGCGGCCGCAGCCGCCGCCTCATCCCCGGCGGGGCTGCTGGCCGGACTGCCACGCTTTA GCAGCCTGAGCCCGCCGCCGCCGCCGCCCGGGCTCTACTTCAGCCCCAGCGCCGCGGCCGTGGCCGCCGT GGGCCGGTACCCCAAGCCGCTGGCTGAGCTGCCTGGCCGGACGCCCATCTTCTGGCCCGGAGTGATGCAG AGCCCGCCCTGGAGGGACGCACGCCTGGCCTGTACCCCTCATCAAGGATCCATTTTGTTGGACAAAGACG GGAAGAGAAAACACACGAGACCCACTTTTTCCGGACAGCAGATCTTCGCCCTGGAGAAGACTTTCGAACA AACAAAATACTTGGCGGGGCCCGAGAGGGCTCGTTTGGCCTATTCGTTGGGGATGACAGAGAGTCAGGTC AAGGTCTGGTTCCAGAACCGCCGGACCAAGTGGAGGAAGAAGCACGCTGCCGAGATGGCCACGGCCAAGA AGAAGCAGGACTCGGAGACAGAGCGCCTCAAGGGGGCCTCGGAGAACGAGGAAGAGGACGACGACTACAA TAAGCCTCTGGATCCCAACTCGGACGACGAGAAAATCACGCAGCTGTTGAAGAAGCACAAGTCCAGCAGC GGCGGCGGCGGCGGCCTCCTACTGCACGCGTCCGAGCCGGAGAGCTCATCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC224762 representing NM_006168
Red=Cloning site Green=Tags(s) MLAVGAMEGTRQSAFLLSSPPLAALHSMAEMKTPLYPAAYPPLPAGPPSSSSSSSSSSSPSPPLGTHNPG GLKPPATGGLSSLGSPPQQLSAATPHGINDILSRPSMPVASGAALPSASPSGSSSSSSSSASASSASAAA AAAAAAAAAASSPAGLLAGLPRFSSLSPPPPPPGLYFSPSAAAVAAVGRYPKPLAELPGRTPIFWPGVMQ SPPWRDARLACTPHQGSILLDKDGKRKHTRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQV KVWFQNRRTKWRKKHAAEMATAKKKQDSETERLKGASENEEEDDDYNKPLDPNSDDEKITQLLKKHKSSS GGGGGLLLHASEPESSS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006168 |
| ORF Size | 1101 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006168.2, NP_006159.2 |
| RefSeq Size | 1116 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 4825 |
| UniProt ID | P78426 |
| Protein Families | Transcription Factors |
| Protein Pathways | Maturity onset diabetes of the young |
| MW | 37.7 kDa |
| Gene Summary | In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Parkinson-associated risk variant in distal enhancer of a-synuclein modulates target gene expression
,Soldner, F;Stelzer, Y;Shivalila, CS;Abraham, BJ;Latourelle, JC;Barrasa, MI;Goldmann, J;Myers, RH;Young, RA;Jaenisch, R;,
Nature
,PubMed ID 27096366
[nkx6.1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224762L1 | Lenti ORF clone of Human NK6 homeobox 1 (NKX6-1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC224762L2 | Lenti ORF clone of Human NK6 homeobox 1 (NKX6-1), mGFP tagged |
CNY 7,888.00 |
|
| RC224762L3 | Lenti ORF clone of Human NK6 homeobox 1 (NKX6-1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224762L4 | Lenti ORF clone of Human NK6 homeobox 1 (NKX6-1), mGFP tagged |
CNY 7,888.00 |
|
| RG224762 | NKX6 (tGFP-tagged) - Human NK6 homeobox 1 (NKX6-1) |
CNY 7,088.00 |
|
| SC303741 | NKX6 (untagged)-Human NK6 homeobox 1 (NKX6-1) |
CNY 5,488.00 |
