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POLE (NM_006231) Human Tagged ORF Clone
CAT＃: RC224742
- TrueORF®
POLE (Myc-DDK-tagged)-Human polymerase (DNA directed), epsilon (POLE)
ORF Plasmid: tGFP
"NM_006231" in other vectors (3)
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CNY 15,088.00
货期*
现货
规格
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经常一起买 (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CRCS12; FILS; IMAGEI; POLE1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224742 representing NM_006231
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGAGGAGCGGCGGGCGGCGGCGCGCGGACCCAGGCGCGGATGGCGAGGCCAGCAGGGATGATG GCGCCACTTCCTCAGTTTCGGCACTCAAGCGCCTGGAACGGAGTCAGTGGACGGATAAGATGGATTTGCG GTTTGGTTTTGAGCGGCTGAAGGAGCCTGGTGAGAAGACAGGCTGGCTCATTAACATGCATCCTACCGAG ATTTTAGATGAAGATAAGCGCTTAGGCAGTGCAGTGGATTACTACTTTATTCAAGATGACGGAAGCAGAT TTAAGGTGGCTTTGCCCTATAAACCGTATTTCTACATTGCGACCAGAAAGGGTTGTGAGCGAGAAGTTTC ATCTTTTCTCTCCAAGAAGTTTCAGGGCAAAATTGCAAAAGTGGAGACTGTCCCCAAAGAGGATCTGGAC TTGCCAAATCACTTGGTGGGTTTGAAGCGAAATTACATCAGGCTGTCCTTCCACACTGTGGAGGATCTTG TCAAAGTGAGGAAGGAGATCTCCCCTGCCGTGAAGAAGAACAGGGAGCAGGATCACGCCAGCGACGCGTA CACAGCTCTGCTTTCCAGTGTTCTGCAGAGGGGCGGTGTCATTACTGATGAAGAGGAAACCTCTAAGAAG ATAGCTGACCAGTTGGACAACATTGTGGACATGCGCGAGTACGATGTTCCCTACCACATCCGCCTCTCCA TTGACCTGAAGATCCACGTGGCTCATTGGTACAATGTCAGATACCGAGGAAATGCTTTTCCGGTAGAAAT CACCCGCCGAGATGACCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATTGAGACGACCAAA CTGCCCCTCAAGTTTCCTGATGCTGAGACAGACCAGATTATGATGATTTCCTACATGATCGATGGCCAGG GCTACCTCATCACCAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTCACCCCCAAGCCAGA ATATGAAGGCCCCTTTTGTGTCTTCAATGAACCCGATGAGGCTCATCTGATCCAAAGGTGGTTTGAACAC GTCCAGGAGACCAAACCCACCATCATGGTCACCTACAACGGGGACTTTTTTGACTGGCCATTTGTGGAGG CCCGGGCAGCAGTCCACGGTCTGAGCATGCAGCAGGAGATAGGCTTCCAGAAGGACAGCCAGGGGGAGTA CAAGGCGCCCCAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC AGTCATAATCTCAAGGCGGCCGCCAAGGCCAAGCTAGGCTATGATCCCGTGGAGCTAGACCCGGAGGACA TGTGCCGGATGGCCACGGAGCAGCCCCAGACTCTGGCCACGTATTCTGTGTCAGATGCTGTCGCCACTTA CTACCTGTACATGAAGTACGTCCACCCATTCATCTTTGCTCTGTGCACCATTATTCCCATGGAGCCCGAC GAGGTGCTGCGGAAGGGCTCTGGCACTCTGTGTGAGGCCTTGCTGATGGTGCAGGCCTTCCACGCCAACA TCATCTTCCCCAACAAGCAAGAGCAGGAGTTCAATAAGCTGACGGACGACGGACACGTGCTGGACTCTGA GACCTACGTCGGGGGCCACGTGGAGGCCCTCGAGTCTGGGGTTTTCCGCAGCGATATCCCTTGCCGGTTT AGGATGAATCCTGCCGCCTTTGACTTCCTGCTGCAGCGGGTTGAGAAGACCTTGCGCCACGCCCTTGAGG AAGAGGAGAAAGTGCCTGTGGAGCAAGTCACCAACTTTGAAGAGGTGTGTGATGAGATTAAGAGCAAGCT TGCCTCCCTGAAGGACGTTCCCAGCCGCATCGAGTGTCCACTCATCTACCACCTGGACGTGGGGGCCATG TACCCCAACATCATCCTGACCAACCGCCTGCAGCCCTCTGCCATGGTGGACGAAGCCACCTGTGCTGCCT GTGACTTCAATAAGCCTGGAGCAAACTGCCAGCGGAAGATGGCCTGGCAGTGGAGGGGCGAGTTCATGCC AGCCAGTCGCAGCGAATACCATCGGATCCAGCACCAGCTGGAGTCAGAGAAGTTCCCCCCCTTGTTCCCA GAGGGGCCAGCTCGGGCCTTTCATGAACTGTCCCGCGAGGAACAGGCGAAATACGAGAAGAGAAGGCTGG CGGATTACTGCCGGAAAGCCTACAAGAAGATCCACATCACCAAGGTGGAAGAGCGTCTCACCACCATCTG CCAGCGGGAAAACTCCTTCTACGTGGACACCGTGCGTGCCTTCCGGGACAGGCGTTACGAGTTCAAAGGG CTCCACAAGGTGTGGAAAAAGAAGCTCTCGGCGGCCGTGGAGGTGGGCGACGCGGCTGAGGTGAAGCGCT GCAAGAACATGGAGGTGCTGTATGACTCGCTGCAGCTGGCCCACAAGTGCATCCTGAACTCCTTCTATGG CTATGTCATGCGCAAGGGGGCTCGCTGGTACTCCATGGAGATGGCTGGCATCGTCTGCTTCACAGGGGCC AACATCATCACCCAGGCACGGGAGCTGATCGAGCAGATTGGGAGGCCCTTAGAGCTGGACACAGATGGTA TATGGTGCGTCCTGCCCAACAGCTTCCCAGAAAATTTTGTCTTCAAGACGACCAATGTGAAGAAGCCCAA AGTGACCATCTCCTACCCAGGCGCCATGTTGAACATCATGGTCAAGGAAGGCTTCACCAATGACCAGTAC CAGGAGCTGGCTGAGCCGTCCTCACTCACCTACGTCACCCGCTCAGAGAACAGCATCTTTTTTGAGGTTG ATGGGCCCTACCTTGCCATGATTCTTCCAGCCTCCAAGGAAGAAGGCAAGAAATTGAAGAAGAGGTATGC TGTGTTCAATGAAGACGGTTCTCTGGCTGAGCTCAAGGGCTTTGAGGTCAAACGCCGCGGGGAACTGCAG CTGATTAAGATCTTCCAATCCTCGGTGTTTGAGGCCTTCCTCAAGGGCAGCACGCTGGAAGAGGTGTATG GCTCTGTAGCCAAGGTGGCTGACTACTGGCTGGACGTGCTGTACAGCAAGGCAGCCAACATGCCTGACTC TGAGCTATTCGAGCTCATCTCTGAGAACCGTTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCAGAAG TCTACGTCCATCAGCACAGCAAAGCGCCTGGCCGAGTTCCTGGGAGACCAGATGGTCAAGGATGCAGGGC TGAGTTGCCGCTACATCATCTCCCGCAAGCCCGAGGGCTCCCCTGTCACGGAGAGGGCCATCCCACTTGC CATTTTCCAAGCAGAGCCCACGGTGAGGAAGCACTTTCTCCGGAAATGGCTCAAGAGCTCTTCCCTTCAA GACTTTGATATTCGAGCAATTCTGGATTGGGACTACTACATTGAGCGGCTGGGAAGCGCCATCCAGAAGA TCATCACCATCCCTGCGGCCCTGCAGCAGGTAAAGAACCCAGTGCCACGTGTCAAACACCCCGACTGGCT GCACAAAAAACTGCTGGAGAAGAATGATGTCTACAAGCAGAAGAAGATCAGTGAGCTCTTCACCCTGGAG GGCAGGAGACAGGTCACGATGGCCGAGGCCTCAGAAGACAGTCCGAGGCCAAGTGCTCCTGACATGGAGG ACTTCGGCCTCGTAAAGCTGCCTCACCCAGCAGCCCCTGTCACTGTGAAGAGGAAGCGAGTTCTTTGGGA GAGCCAGGAGGAGTCCCAGGACCTCACGCCGACTGTGCCCTGGCAGGAAATCTTGGGGCAGCCTCCCGCC CTGGGAACCAGCCAGGAGGAATGGCTTGTCTGGCTCCGGTTCCACAAGAAGAAGTGGCAGCTGCAGGCCC GGCAGCGCCTCGCCCGCAGGAAGAGGCAGCGTCTGGAGTCGGCAGAGGGTGTGCTCAGGCCCGGGGCCAT CCGGGATGGTCCTGCCACGGGGCTGGGGAGCTTCTTGCGAAGAACTGCCCGCAGCATCCTGGACCTTCCG TGGCAGATTGTGCAGATCAGCGAGACCAGCCAGGCCGGCCTGTTCAGGCTGTGGGCGCTCGTTGGCAGTG ACTTGCACTGCATCAGGCTGAGCATCCCCCGTGTGTTCTACGTGAACCAGCGAGTCGCTAAAGCGGAGGA GGGTGCTTCGTATCGCAAGGTAAATCGGGTCCTTCCTCGCTCCAACATGGTCTACAATCTCTATGAGTAT TCAGTGCCAGAGGACATGTACCAGGAACACATCAACGAGATCAACGCTGAGCTGTCAGCGCCAGACATCG AGGGCGTATATGAGACTCAGGTTCCGTTACTGTTCCGGGCCCTGGTGCACCTGGGCTGTGTGTGTGTGGT CAATAAACAGCTGGTGAGGCACCTTTCAGGCTGGGAAGCAGAGACCTTTGCTCTTGAGCACCTGGAGATG CGCTCTCTGGCCCAGTTCAGCTACCTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCACCACGCAC AGGCCCACAAAGCGCTCTTCGGGATCTTCATCCCCTCACAGCGCAGGGCATCCGTCTTTGTGCTGGACAC TGTGCGCAGCAACCAGATGCCCAGCCTTGGCGCCCTGTACTCAGCAGAGCACGGCCTCCTCCTGGAGAAG GTGGGCCCTGAGCTCCTGCCACCCCCCAAACACACCTTCGAAGTTCGGGCAGAAACTGACCTGAAGACCA TCTGCAGAGCCATCCAGCGATTCCTGCTCGCCTACAAGGAGGAGCGCCGGGGGCCCACACTCATCGCTGT TCAGTCCAGCTGGGAGCTGAAGAGGCTGGCCAGTGAAATTCCTGTCTTGGAGGAATTCCCACTGGTGCCT ATCTGTGTGGCTGACAAGATCAACTATGGGGTCCTGGACTGGCAGCGCCATGGAGCCCGGCGCATGATCC GTCACTACCTCAACCTGGACACCTGCCTGTCGCAGGCCTTCGAGATGAGCAGGTACTTTCACATTCCCAT TGGGAACCTACCAGAGGACATCTCCACATTCGGCTCCGACCTCTTCTTTGCCCGCCACCTCCAGCGCCAC AACCACCTGCTCTGGCTGTCCCCTACAGCCCGCCCTGACCTGGGTGGAAAGGAGGCTGATGACAACTGTC TTGTCATGGAGTTCGATGACCAAGCCACTGTTGAGATCAACAGTTCAGGCTGTTACTCCACAGTGTGTGT GGAGCTGGACCTTCAGAACCTGGCCGTCAACACCATTCTCCAGTCTCACCATGTCAACGACATGGAGGGG GCCGACAGCATGGGGATCAGCTTCGACGTGATCCAGCAGGCCTCCCTGGAGGACATGATCACGGGTGGTC AGGCTGCCAGTGCCCCGGCCAGCTACGATGAGACAGCCCTGTGCTCTAACACCTTCAGGATCCTGAAGAG CATGGTCGTGGGCTGGGTGAAGGAGATCACCCAGTACCACAACATCTATGCAGACAACCAGGTGATGCAC TTCTACCGCTGGCTTCGGTCGCCATCCTCTCTGCTTCATGACCCTGCCCTGCACCGCACACTCCACAACA TGATGAAGAAGCTCTTCCTGCAGCTCATCGCTGAGTTCAAGCGCCTGGGGTCATCAGTCATCTACGCCAA CTTCAACCGCATCATCCTCTGTACAAAGAAGCGCCGTGTGGAAGATGCCATCGCTTACGTGGAGTACATC ACCAGCAGCATCCATTCAAAGGAGACCTTCCATTCTCTGACAATTTCTTTCTCTCGATGCTGGGAATTTC TTCTCTGGATGGATCCATCTAACTATGGCGGAATCAAAGGAAAAGTTTCATCTCGTATTCACTGTGGACT GCAAGACTCCCAGAAAGCAGGGGGAGCAGAGGATGAGCAGGAAAATGAGGACGATGAGGAGGAAAGAGAT GGGGAGGAGGAGGAAGAGGCGGAGGAATCCAACGTGGAGGATTTACTGGAAAACAACTGGAACATTTTGC AGTTTTTGCCACAGGCAGCCTCCTGCCAGAACTACTTCCTCATGATTGTTTCAGCGTACATCGTGGCCGT GTACCACTGCATGAAGGACGGGCTGAGGCGCAGTGCTCCAGGGAGCACCCCCGTGAGGAGGAGGGGGGCC AGCCAGCTCTCCCAGGAGGCCGAGGGGGCGGTCGGAGCCCTTCCCGGAATGATCACCTTCTCTCAGGATT ATGTCGCAAATGAGCTCACTCAGAGCTTCTTCACCATCACTCAGAAGATTCAGAAGAAAGTCACAGGCTC TCGGAACTCCACTGAGCTCTCAGAGATGTTTCCTGTCCTCCCCGGTTCCCACTTGCTGCTCAATAACCCT GCCCTGGAGTTCATCAAATACGTGTGCAAGGTGCTGTCCCTGGACACCAACATCACAAACCAGGTGAATA AGCTGAACCGAGACCTGCTTCGCCTGGTGGATGTCGGCGAGTTCTCCGAGGAGGCCCAGTTCCGAGACCC CTGCCGCTCCTACGTGCTTCCTGAGGTCATCTGCCGCAGCTGTAACTTCTGCCGCGACCTGGACCTGTGT AAAGACTCTTCCTTCTCAGAGGATGGGGCGGTCCTGCCTCAGTGGCTCTGCTCCAACTGTCAGGCGCCCT ACGACTCCTCTGCCATCGAGATGACGCTGGTGGAAGTTCTACAGAAGAAGCTGATGGCCTTCACCCTGCA GGACCTGGTCTGCCTGAAGTGCCGCGGGGTGAAGGAGACCAGCATGCCTGTGTACTGCAGCTGCGCGGGA GACTTCGCCCTCACCATCCACACCCAGGTCTTCATGGAACAGATCGGAATATTCCGGAACATTGCCCAGC ACTACGGCATGTCGTACCTCCTGGAGACCCTGGAGTGGCTGCTGCAGAAGAACCCACAGCTGGGCCAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC224742 representing NM_006231
Red=Cloning site Green=Tags(s) MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTE ILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLD LPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKK IADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTK LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEH VQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVG SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPD EVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRF RMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAM YPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFP EGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKG LHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGA NIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQY QELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQ LIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQK STSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQ DFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLE GRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPA LGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLP WQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEY SVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEM RSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEK VGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVP ICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRH NHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEG ADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMH FYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYI TSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERD GEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGA SQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNP ALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLC KDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLVCLKCRGVKETSMPVYCSCAG DFALTIHTQVFMEQIGIFRNIAQHYGMSYLLETLEWLLQKNPQLGH SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006231 |
| ORF Size | 6858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006231.4 |
| RefSeq Size | 7859 bp |
| RefSeq ORF | 6861 bp |
| Locus ID | 5426 |
| UniProt ID | Q07864 |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
| MW | 261.3 kDa |
| Gene Summary | This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224742L1 | Lenti ORF clone of Human polymerase (DNA directed), epsilon (POLE), Myc-DDK-tagged |
CNY 17,488.00 |
|
| RG224742 | POLE (tGFP-tagged) - Human polymerase (DNA directed), epsilon (POLE) |
CNY 16,688.00 |
|
| SC303754 | POLE (untagged)-Human polymerase (DNA directed), epsilon (POLE) |
CNY 44,080.00 |
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