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GUCY2F (NM_001522) Human Tagged ORF Clone
CAT＃: RC224440
GUCY2F (Myc-DDK-tagged)-Human guanylate cyclase 2F, retinal (GUCY2F)
ORF Plasmid: tGFP
"NM_001522" in other vectors (6)
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CNY 7,712.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CYGF; GC-F; GUC2DL; GUC2F; RETGC-2; ROS-GC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224440 representing NM_001522
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTGGGACTCGGGCGCTTTTCTCGCCTTGTTCTCTGGTTTGCGGCTTTCAGGAAACTGCTGGGAC ACCATGGCCTTGCATCTGCCAAGTTCCTGTGGTGCTTGTGCCTTCTGTCTGTCATGTCCCTTCCGCAGCA GGTGTGGACACTCCCCTACAAGATAGGGGTGGTGGGCCCTTGGGCTTGTGATTCGCTGTTTTCAAAGGCC CTGCCTGAGGTTGCTGCGCGATTAGCCATTGAGCGAATCAACCGGGACCCATCTTTTGACCTGAGTTATT CTTTTGAATACGTGATTCTCAATGAAGACTGCCAGACTTCGAGGGCTCTCTCCAGTTTCATTTCCCACCA CCAGATGGCCTCAGGATTTATTGGACCTACCAACCCTGGCTACTGCGAGGCAGCCTCGCTCCTGGGAAAC AGCTGGGACAAAGGAATTTTCTCTTGGGCTTGTGTGAATTATGAATTAGACAATAAAATTAGCTACCCGA CCTTTTCTCGGACACTCCCTTCTCCCATCCGGGTGCTTGTAACTGTCATGAAATATTTCCAGTGGGCTCA TGCTGGAGTCATTTCCTCAGATGAAGACATTTGGGTGCATACAGCCAATCGAGTCGCAAGTGCTCTTCGG AGCCACGGCTTACCTGTAGGGGTCGTCCTGACCACAGGACAAGACAGCCAAAGCATGCGGAAAGCCCTCC AGAGGATTCACCAGGCAGACAGAATTCGCATAATCATCATGTGTATGCATTCAGCTTTGATTGGGGGAGA GACTCAGATGCATCTCTTGGAATGTGCTCATGATCTGAAAATGACTGATGGAACCTACGTCTTTGTTCCT TATGATGCCCTGCTCTACAGTTTACCTTATAAGCACACCCCCTACCGGGTCCTAAGGAACAACCCAAAGC TCCGGGAAGCCTATGATGCAGTGTTGACCATTACAGTGGAGTCCCAAGAAAAGACCTTCTATCAAGCCTT CACAGAGGCAGCAGCAAGAGGTGAAATTCCTGAGAAGCTGGAGTTCGATCAAGTTTCACCGTTGTTTGGA ACCATCTACAATTCAATTTACTTTATCGCACAAGCCATGAATAATGCTATGAAAGAAAATGGACAGGCTG GTGCTGCCAGCCTGGTTCAGCATTCCAGAAACATGCAGTTCCATGGATTCAACCAGTTGATGAGGACAGA TTCAAATGGAAATGGAATTTCAGAATATGTAATCCTGGACACCAACTTGAAAGAATGGGAACTCCATAGC ACCTACACTGTGGACATGGAAATGGAGCTGCTACGTTTCGGAGGGACCCCTATTCACTTCCCTGGTGGCA GGCCCCCTAGAGCAGATGCAAAATGCTGGTTTGCAGAAGGGAAGATCTGCCATGGAGGCATCGACCCTGC CTTTGCCATGATGGTCTGCCTTACTTTGCTTATAGCCCTGCTGTCTATTAATGGATTTGCTTACTTTATA AGGCGTCGTATAAATAAAATCCAGTTGATCAAAGGACCCAATAGAATTCTACTGACTTTGGAGGATGTAA CGTTTATCAATCCCCACTTTGGCAGTAAGAGAGGAAGTCGTGCCAGTGTAAGCTTCCAGATTACCTCAGA GGTCCAAAGTGGGAGGTCCCCAAGACTCTCCTTTTCTTCAGGGAGTCTAACTCCAGCTACCTATGAAAAC TCCAACATAGCGATTTATGAGGGTGATTGGGTGTGGCTGAAAAAGTTCTCCCTTGGAGATTTTGGAGACC TTAAGTCCATCAAATCAAGAGCAAGTGATGTGTTCGAAATGATGAAGGACTTGCGTCATGAGAATATTAA CCCTTTATTGGGTTTCTTCTATGATTCGGGGATGTTTGCCATTGTGACAGAATTCTGTTCCCGAGGGAGC CTAGAAGACATACTGACAAATCAAGATGTGAAACTTGACTGGATGTTTAAATCATCACTCTTGCTGGATC TCATAAAGGGCATGAAGTACTTACACCACAGAGAGTTTGTTCATGGGAGGCTAAAGTCTCGAAACTGTGT GGTAGATGGGCGTTTTGTACTAAAAGTGACAGATTATGGCTTTAACGACATCTTAGAAATGCTGAGACTC TCTGAAGAGGAATCTTCTATGGAAGAGCTGCTGTGGACGGCCCCTGAACTGTTGAGAGCTCCAAGAGGCA GCAGGTTAGGTTCTTTTGCAGGAGATGTCTATAGCTTTGCCATCATCATGCAAGAAGTGATGGTCCGGGG TACCCCATTCTGCATGATGGATCTGCCAGCTCAAGAAATCATAAACAGACTTAAGAAGCCTCCTCCTGTG TACAGACCAGTAGTTCCTCCTGAGCATGCCCCTCCAGAATGTCTCCAGCTGATGAAGCAGTGCTGGGCTG AGGCTGCAGAACAACGACCAACTTTTGATGAAATATTTAACCAGTTTAAAACTTTTAATAAAGGGAAGAA GACCAATATTATTGATTCTATGCTTCGGATGTTGGAGCAATATTCTAGCAACTTGGAAGATTTGATTCGG GAGCGGACTGAAGAGCTGGAAATTGAAAAACAGAAAACGGAAAAGCTTCTAACACAGATGCTACCACCAT CAGTTGCTGAATCTCTCAAAAAGGGCTGCACAGTTGAACCTGAGGGCTTTGACTTGGTCACCTTGTACTT CAGCGACATTGTGGGCTTCACAACCATTTCAGCCATGAGTGAGCCCATTGAGGTCGTGGATCTTCTGAAT GACCTGTACACACTCTTTGATGCAATAATTGGCAGTCATGATGTCTACAAGGTAGAGACCATTGGAGATG CCTACATGGTGGCTTCAGGCCTCCCAAAGAGGAATGGCAGTAGGCATGCAGCTGAGATTGCAAACATGTC CTTAGATATCCTGAGCTCTGTGGGCACTTTCAAGATGCGGCACATGCCAGAAGTGCCGGTCCGAATTCGA ATTGGCCTTCACTCAGGGCCGGTTGTTGCTGGAGTGGTGGGCCTCACCATGCCCAGATACTGCTTGTTTG GAGACACTGTGAACACAGCTTCTCGGATGGAATCTACAGGCTTACCTTATCGCATTCATGTCAGTCTCAG CACTGTTACAATTCTTCAAAATCTGAGTGAGGGCTATGAAGTGGAGCTTCGAGGAAGAACAGAGCTCAAG GGCAAAGGCACAGAGGAAACCTTCTGGCTGATTGGGAAAAAAGGCTTCATGAAGCCCCTTCCTGTGCCCC CACCAGTGGACAAAGATGGGCAAGTGGGCCATGGCCTGCAACCAGTGGAGATTGCAGCCTTCCAAAGAAG AAAAGCAGAAAGGCAGTTGGTGAGAAACAAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224440 representing NM_001522
Red=Cloning site Green=Tags(s) MFLGLGRFSRLVLWFAAFRKLLGHHGLASAKFLWCLCLLSVMSLPQQVWTLPYKIGVVGPWACDSLFSKA LPEVAARLAIERINRDPSFDLSYSFEYVILNEDCQTSRALSSFISHHQMASGFIGPTNPGYCEAASLLGN SWDKGIFSWACVNYELDNKISYPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWVHTANRVASALR SHGLPVGVVLTTGQDSQSMRKALQRIHQADRIRIIIMCMHSALIGGETQMHLLECAHDLKMTDGTYVFVP YDALLYSLPYKHTPYRVLRNNPKLREAYDAVLTITVESQEKTFYQAFTEAAARGEIPEKLEFDQVSPLFG TIYNSIYFIAQAMNNAMKENGQAGAASLVQHSRNMQFHGFNQLMRTDSNGNGISEYVILDTNLKEWELHS TYTVDMEMELLRFGGTPIHFPGGRPPRADAKCWFAEGKICHGGIDPAFAMMVCLTLLIALLSINGFAYFI RRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQITSEVQSGRSPRLSFSSGSLTPATYEN SNIAIYEGDWVWLKKFSLGDFGDLKSIKSRASDVFEMMKDLRHENINPLLGFFYDSGMFAIVTEFCSRGS LEDILTNQDVKLDWMFKSSLLLDLIKGMKYLHHREFVHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRL SEEESSMEELLWTAPELLRAPRGSRLGSFAGDVYSFAIIMQEVMVRGTPFCMMDLPAQEIINRLKKPPPV YRPVVPPEHAPPECLQLMKQCWAEAAEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIR ERTEELEIEKQKTEKLLTQMLPPSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLN DLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIR IGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQNLSEGYEVELRGRTELK GKGTEETFWLIGKKGFMKPLPVPPPVDKDGQVGHGLQPVEIAAFQRRKAERQLVRNKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001522 |
| ORF Size | 3324 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001522.3 |
| RefSeq Size | 3723 bp |
| RefSeq ORF | 3327 bp |
| Locus ID | 2986 |
| UniProt ID | P51841 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Purine metabolism |
| MW | 124.7 kDa |
| Gene Summary | The protein encoded by this gene is a guanylyl cyclase found predominantly in photoreceptors in the retina. The encoded protein is thought to be involved in resynthesis of cGMP after light activation of the visual signal transduction cascade, allowing a return to the dark state. This protein is a single-pass type I membrane protein. Defects in this gene may be a cause of X-linked retinitis pigmentosa. [provided by RefSeq, Dec 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224440L1 | Lenti ORF clone of Human guanylate cyclase 2F, retinal (GUCY2F), Myc-DDK-tagged |
CNY 10,112.00 |
|
| RC224440L2 | Lenti ORF clone of Human guanylate cyclase 2F, retinal (GUCY2F), mGFP tagged |
CNY 12,920.00 |
|
| RC224440L3 | Lenti ORF clone of Human guanylate cyclase 2F, retinal (GUCY2F), Myc-DDK-tagged |
CNY 12,920.00 |
|
| RC224440L4 | Lenti ORF clone of Human guanylate cyclase 2F, retinal (GUCY2F), mGFP tagged |
CNY 12,920.00 |
|
| RG224440 | GUCY2F (tGFP-tagged) - Human guanylate cyclase 2F, retinal (GUCY2F) |
CNY 12,160.00 |
|
| SC303040 | GUCY2F (untagged)-Human guanylate cyclase 2F, retinal (GUCY2F) |
CNY 7,720.00 |
