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PIWIL3 (NM_001008496) Human Tagged ORF Clone
CAT＃: RC224247
PIWIL3 (Myc-DDK-tagged)-Human piwi-like 3 (Drosophila) (PIWIL3)
ORF Plasmid: tGFP
"NM_001008496" in other vectors (6)
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CNY 6,464.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HIWI3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224247 representing NM_001008496
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGTAGGGCAAGGACTCGCGCCCGAGGCAGAGCCCGCCGCAGGGAGAGCTACCAACAAGAGGCAC CTGGGGGACCCAGAGCACCTGGATCAGCTACAACCCAGGAGCCCCCTCAGTTGCAGTCGACACCCCGGCC GCTGCAGGAGGAAGTCCCAGTGGTTAGACCTCTGCAGCCAAGAGCAGCAAGAGGAGGAGCAGGAGGAGGA GCACAGTCTCAAGGGGTGAAGGAACCTGGACCTGAGGCTGGGTTGCATACAGCGCCCTTGCAGGAGAGAA GGATTGGTGGAGTTTTTCAAGACCTGGTGGTGAACACCAGGCAAGATATGAAGCATGTTAAAGACTCAAA AACAGGTTCAGAGGGTACAGTGGTACAGCTACTCGCCAACCACTTCCGAGTGATATCTCGTCCTCAGTGG GTTGCATATAAATACAACGTTGACTACAAACCAGACATAGAAGATGGAAATCTCCGTACAATTTTACTTG ATCAACATAGAAGGAAATTTGGAGAGCGCCATATATTTGATGGAAACTCTTTATTATTATCTCGGCCACT AAAAGAGCGGAGAGTGGAATGGTTGAGCACAACCAAAGACAAAAACATCGTGAAGATTACAGTTGAGTTT TCCAAAGAACTCACGCCCACGTCGCCAGATTGCCTACGCTATTACAACATTCTCTTTAGAAGAACTTTCA AGCTGCTGGATTTTGAACAAGTTGGTCGCAACTATTATACCAAAAAGAAGGCCATTCAGTTATACCGTCA TGGTACCAGTTTGGAAATCTGGCTTGGTTATGTTACTTCTGTTCTTCAATACGAAAACAGCATTACCCTC TGTGCCGATGTGAGCCACAAACTGCTCCGAATAGAAACTGCTTATGATTTCATAAAGAGAACATCTGCCC AGGCCCAGACAGGAAACATCCGAGAGGAAGTAACTAATAAATTAATTGGATCAATTGTTCTGACAAAATA CAACAACAAAACCTACAGAGTAGATGATATTGATTGGAAGCAGAATCCTGAAGACACATTTAACAAATCA GATGGCAGCAAAATCACCTATATAGACTACTACAGGCAGCAACATAAAGAAATTGTCACAGTGAAGAAAC AGCCACTTTTGGTCAGCCAGGGCAGATGGAAAAAGGGCCTAACGGGTACACAACGTGAACCTATCCTGCT GATTCCTCAGCTGTGCCACATGACAGGTCTAACAGATGAAATATGTAAAGATTATAGCATTGTGAAAGAA TTGGCTAAACATACAAGATTGAGTCCAAGAAGAAGGCATCATACATTAAAAGAATTCATCAATACTCTAC AAGATAATAAAAAAGTACGAGAGTTACTTCAACTCTGGGATTTGAAATTTGATACCAATTTTTTGTCCGT CCCGGGAAGAGTTTTGAAAAACGCAAACATCGTGCAAGGCAGAAGAATGGTTAAAGCCAATTCACAAGGA GACTGGTCAAGAGAAATAAGAGAATTACCCTTACTTAATGCAATGCCACTACATAGTTGGCTCATACTCT ATAGCAGGAGCAGTCACAGAGAAGCCATGTCCTTAAAGGGTCATCTACAGAGTGTCACAGCCCCCATGGG CATAACTATGAAACCAGCAGAAATGATTGAAGTAGATGGTGATGCTAACTCCTATATAGACACATTACGG AAATATACTAGACCAACACTGCAGATGGGAATGTCATGTTTGCTGGTTTTCAAGGTGATTTGTATCCTGC CCAATGATGACAAACGTAGATATGACAGCATAAAAAGATACCTATGTACCAAATGCCCAATTCCAAGCCA GTGTGTGGTGAAAAAGACCTTAGAAAAAGTCCAGGCAAGGACCATCGTCACCAAGATTGCCCAGCAGATG AATTGCAAGATGGGAGGAGCCCTCTGGAAGGTGGAGACAGACGTACAAAGAACAATGTTCGTTGGCATTG ATTGTTTCCACGATATCGTAAATCGACAGAAATCAATAGCAGGATTTGTTGCAAGTACCAATGCTGAATT AACAAAGTGGTACTCTCAATGTGTCATCCAGAAAACAGGAGAAGAGCTTGTGAAAGAGCTGGAGATCTGC TTGAAAGCTGCCCTGGATGTCTGGTGTAAAAACGAATCATCGATGCCACATTCTGTTATTGTGTATCGGG ATGGAGTGGGAGATGGTCAGCTTCAAGCATTGCTTGACCATGAAGCGAAAAAGATGTCGACCTACTTAAA AACCATCTCTCCTAACAATTTCACTCTAGCTTTCATTGTGGTGAAGAAACGAATAAACACTAGATTTTTT CTTAAACATGGAAGCAATTTTCAAAATCCACCTCCAGGAACAGTTATTGATGTAGAGTTGACTAGGAATG AATGGTATGACTTTTTTATTGTGAGTCAGTCTGTGCAAGATGGGACTGTTACCCCCACTCATTATAACGT CATCTATGACACGATTGGCTTGAGCCCAGATACAGTACAGCGTTTAACATATTGTCTATGCCACATGTAT TATAATTTGCCAGGCATCATCCGAGTTCCAGCGCCTTGCCACTATGCCCACAAGCTGGCTTACCTCGTGG GGCAGTCCATTCACCAGGAACCGAATCGTTCCTTGTCAACTCGTCTCTTTTACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224247 representing NM_001008496
Red=Cloning site Green=Tags(s) MPGRARTRARGRARRRESYQQEAPGGPRAPGSATTQEPPQLQSTPRPLQEEVPVVRPLQPRAARGGAGGG AQSQGVKEPGPEAGLHTAPLQERRIGGVFQDLVVNTRQDMKHVKDSKTGSEGTVVQLLANHFRVISRPQW VAYKYNVDYKPDIEDGNLRTILLDQHRRKFGERHIFDGNSLLLSRPLKERRVEWLSTTKDKNIVKITVEF SKELTPTSPDCLRYYNILFRRTFKLLDFEQVGRNYYTKKKAIQLYRHGTSLEIWLGYVTSVLQYENSITL CADVSHKLLRIETAYDFIKRTSAQAQTGNIREEVTNKLIGSIVLTKYNNKTYRVDDIDWKQNPEDTFNKS DGSKITYIDYYRQQHKEIVTVKKQPLLVSQGRWKKGLTGTQREPILLIPQLCHMTGLTDEICKDYSIVKE LAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGRRMVKANSQG DWSREIRELPLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDGDANSYIDTLR KYTRPTLQMGMSCLLVFKVICILPNDDKRRYDSIKRYLCTKCPIPSQCVVKKTLEKVQARTIVTKIAQQM NCKMGGALWKVETDVQRTMFVGIDCFHDIVNRQKSIAGFVASTNAELTKWYSQCVIQKTGEELVKELEIC LKAALDVWCKNESSMPHSVIVYRDGVGDGQLQALLDHEAKKMSTYLKTISPNNFTLAFIVVKKRINTRFF LKHGSNFQNPPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDTIGLSPDTVQRLTYCLCHMY YNLPGIIRVPAPCHYAHKLAYLVGQSIHQEPNRSLSTRLFYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008496 |
| ORF Size | 2646 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008496.3, NP_001008496.2 |
| RefSeq Size | 3504 bp |
| RefSeq ORF | 2649 bp |
| Locus ID | 440822 |
| UniProt ID | Q7Z3Z3 |
| Protein Pathways | Dorso-ventral axis formation |
| MW | 100.9 kDa |
| Gene Summary | This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224247L1 | Lenti ORF clone of Human piwi-like 3 (Drosophila) (PIWIL3), Myc-DDK-tagged |
CNY 8,864.00 |
|
| RC224247L2 | Lenti ORF clone of Human piwi-like 3 (Drosophila) (PIWIL3), mGFP tagged |
CNY 9,030.00 |
|
| RC224247L3 | Lenti ORF clone of Human piwi-like 3 (Drosophila) (PIWIL3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC224247L4 | Lenti ORF clone of Human piwi-like 3 (Drosophila) (PIWIL3), mGFP tagged |
CNY 9,030.00 |
|
| RG224247 | PIWIL3 (tGFP-tagged) - Human piwi-like 3 (Drosophila) (PIWIL3) |
CNY 7,890.00 |
|
| SC301309 | PIWIL3 (untagged)-Human piwi-like 3 (Drosophila) (PIWIL3) |
CNY 17,010.00 |
