GNAT3 (NM_001102386) Human Tagged ORF Clone

CAT#: RC224201

GNAT3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3)

ORF Plasmid: DDK tGFP



  "NM_001102386" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-GNAT3 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GDCA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224201 representing NM_001102386
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAGTGGAATTAGTTCAGAGAGCAAGGAGTCAGCCAAAAGATCAAAAGAACTGGAGAAAAAGCTTC
AGGAGGATGCTGAGCGAGATGCAAGAACCGTAAAGCTGCTACTATTAGGAGCAGGAGAATCTGGGAAAAG
TACTATTGTTAAACAAATGAAGATCATCCATAAGAATGGTTACAGTGAGCAAGAATGCATGGAGTTCAAA
GCAGTAATTTACAGTAATACATTGCAATCCATCCTAGCTATTGTGAAAGCCATGACTACCCTTGGAATTG
ATTATGTAAATCCCAGAAGTGCAGAGGACCAACGACAACTTTATGCAATGGCAAATACCCTGGAAGATGG
TGGCATGACACCTCAACTGGCTGAGGTAATAAAACGGCTGTGGAGAGATCCAGGAATTCAGGCCTGCTTT
GAAAGGGCATCTGAATATCAGCTCAATGACTCAGCAGCTTACTACCTTAATGATTTAGATAGAATAACAG
CATCTGGGTATGTGCCAAATGAACAAGATGTTCTCCATTCTCGAGTGAAAACGACTGGAATCATTGAAAC
TCAATTCTCCTTTAAAGACTTGCACTTCAGGATGTTTGATGTAGGTGGACAGAGATCTGAGAGAAAGAAG
TGGATTCACTGCTTTGAAGGAGTTACATGCATTATATTTTGTGCTGCACTTAGTGCCTATGACATGGTCC
TCGTGGAAGACGAAGAAGTGAATAGAATGCATGAAAGCCTTCACCTGTTCAACAGTATCTGTAATCACAA
GTATTTTTCAACAACCTCCATTGTCCTGTTCCTCAACAAAAAAGATATCTTTCAAGAAAAGGTAACCAAG
GTGCATCTTAGTATCTGCTTTCCAGAATACACTGGGCCAAATACATTTGAAGATGCAGGAAACTACATCA
AGAACCAGTTTCTAGACCTGAATTTAAAAAAAGAAGATAAGGAAATTTATTCCCACATGACCTGTGCTAC
TGACACCCAAAATGTCAAGTTTGTGTTTGACGCAGTTACAGATATAATAATCAAAGAGAATCTAAAAGAC
TGTGGGCTTTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224201 representing NM_001102386
Red=Cloning site Green=Tags(s)

MGSGISSESKESAKRSKELEKKLQEDAERDARTVKLLLLGAGESGKSTIVKQMKIIHKNGYSEQECMEFK
AVIYSNTLQSILAIVKAMTTLGIDYVNPRSAEDQRQLYAMANTLEDGGMTPQLAEVIKRLWRDPGIQACF
ERASEYQLNDSAAYYLNDLDRITASGYVPNEQDVLHSRVKTTGIIETQFSFKDLHFRMFDVGGQRSERKK
WIHCFEGVTCIIFCAALSAYDMVLVEDEEVNRMHESLHLFNSICNHKYFSTTSIVLFLNKKDIFQEKVTK
VHLSICFPEYTGPNTFEDAGNYIKNQFLDLNLKKEDKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKD
CGLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001102386
ORF Size 1062 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001102386.3
RefSeq Size 1065 bp
RefSeq ORF 1065 bp
Locus ID 346562
UniProt ID A8MTJ3
Protein Pathways Taste transduction
MW 40.2 kDa
Gene Summary Sweet, bitter, and umami tastes are transmitted from taste receptors by a specific guanine nucleotide binding protein. The protein encoded by this gene is the alpha subunit of this heterotrimeric G protein, which is found not only in the oral epithelium but also in gut tissues. Variations in this gene have been linked to metabolic syndrome. [provided by RefSeq, Dec 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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