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Factor XII (F12) (NM_000505) Human Tagged ORF Clone
CAT＃: RC224108
- TrueORF®
F12 (Myc-DDK-tagged)-Human coagulation factor XII (Hageman factor) (F12)
ORF Plasmid: tGFP
"NM_000505" in other vectors (6)
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CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HAE3; HAEX; HAF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224108 representing NM_000505
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCTCTGCTGCTCCTGGGGTTCCTGCTGGTGAGCTTGGAGTCAACACTTTCGATTCCACCTTGGG AAGCCCCCAAGGAGCATAAGTACAAAGCTGAAGAGCACACAGTCGTTCTCACTGTCACCGGGGAGCCCTG CCACTTCCCCTTCCAGTACCACCGGCAGCTGTACCACAAATGTACCCACAAGGGCCGGCCAGGCCCTCAG CCCTGGTGTGCTACCACCCCCAACTTTGATCAGGACCAGCGATGGGGATACTGTTTGGAGCCCAAGAAAG TGAAAGACCACTGCAGCAAACACAGCCCCTGCCAGAAAGGAGGGACCTGTGTGAACATGCCAAGCGGCCC CCACTGTCTCTGTCCACAACACCTCACTGGAAACCACTGCCAGAAAGAGAAGTGCTTTGAGCCTCAGCTT CTCCGGTTTTTCCACAAGAATGAGATATGGTATAGAACTGAGCAAGCAGCTGTGGCCAGATGCCAGTGCA AGGGTCCTGATGCCCACTGCCAGCGGCTGGCCAGCCAGGCCTGCCGCACCAACCCGTGCCTCCATGGGGG TCGCTGCCTAGAGGTGGAGGGCCACCGCCTGTGCCACTGCCCGGTGGGCTACACCGGACCCTTCTGCGAC GTGGACACCAAGGCAAGCTGCTATGATGGCCGCGGGCTCAGCTACCGCGGCCTGGCCAGGACCACGCTCT CGGGTGCGCCCTGTCAGCCGTGGGCCTCGGAGGCCACCTACCGGAACGTGACTGCCGAGCAAGCGCGGAA CTGGGGACTGGGCGGCCACGCCTTCTGCCGGAACCCGGACAACGACATCCGCCCGTGGTGCTTCGTGCTG AACCGCGACCGGCTGAGCTGGGAGTACTGCGACCTGGCACAGTGCCAGACCCCAACCCAGGCGGCGCCTC CGACCCCGGTGTCCCCTAGGCTTCATGTCCCACTCATGCCCGCGCAGCCGGCACCGCCGAAGCCTCAGCC CACGACCCGGACCCCGCCTCAGTCCCAGACCCCGGGAGCCTTGCCGGCGAAGCGGGAGCAGCCGCCTTCC CTGACCAGGAACGGCCCACTGAGCTGCGGGCAGCGGCTCCGCAAGAGTCTGTCTTCGATGACCCGCGTCG TTGGCGGGCTGGTGGCGCTACGCGGGGCGCACCCCTACATCGCCGCGCTGTACTGGGGCCACAGTTTCTG CGCCGGCAGCCTCATCGCCCCCTGCTGGGTGCTGACGGCCGCTCACTGCCTGCAGGACCGGCCCGCACCC GAGGATCTGACGGTGGTGCTCGGCCAGGAACGCCGTAACCACAGCTGTGAGCCGTGCCAGACGTTGGCCG TGCGCTCCTACCGCTTGCACGAGGCCTTCTCGCCCGTCAGCTACCAGCACGACCTGGCTCTGTTGCGCCT TCAGGAGGATGCGGACGGCAGCTGCGCGCTCCTGTCGCCTTACGTTCAGCCGGTGTGCCTGCCAAGCGGC GCCGCGCGACCCTCCGAGACCACGCTCTGCCAGGTGGCCGGCTGGGGCCACCAGTTCGAGGGGGCGGAGG AATATGCCAGCTTCCTGCAGGAGGCGCAGGTACCGTTCCTCTCCCTGGAGCGCTGCTCAGCCCCGGACGT GCACGGATCCTCCATCCTCCCCGGCATGCTCTGCGCAGGGTTCCTCGAGGGCGGCACCGATGCGTGCCAG GGTGATTCCGGAGGCCCGCTGGTGTGTGAGGACCAAGCTGCAGAGCGCCGGCTCACCCTGCAAGGCATCA TCAGCTGGGGATCGGGCTGTGGTGACCGCAACAAGCCAGGCGTCTACACCGATGTGGCCTACTACCTGGC CTGGATCCGGGAGCACACCGTTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224108 representing NM_000505
Red=Cloning site Green=Tags(s) MRALLLLGFLLVSLESTLSIPPWEAPKEHKYKAEEHTVVLTVTGEPCHFPFQYHRQLYHKCTHKGRPGPQ PWCATTPNFDQDQRWGYCLEPKKVKDHCSKHSPCQKGGTCVNMPSGPHCLCPQHLTGNHCQKEKCFEPQL LRFFHKNEIWYRTEQAAVARCQCKGPDAHCQRLASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGPFCD VDTKASCYDGRGLSYRGLARTTLSGAPCQPWASEATYRNVTAEQARNWGLGGHAFCRNPDNDIRPWCFVL NRDRLSWEYCDLAQCQTPTQAAPPTPVSPRLHVPLMPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPS LTRNGPLSCGQRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPAP EDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALLSPYVQPVCLPSG AARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQ GDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000505 |
| ORF Size | 1845 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000505.2, NP_000496.1 |
| RefSeq Size | 2048 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 2161 |
| UniProt ID | P00748 |
| Domains | KR, FN1, FN2, Tryp_SPc, EGF, EGF |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades |
| MW | 67.82 kDa |
| Gene Summary | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224108L1 | Lenti ORF clone of Human coagulation factor XII (Hageman factor) (F12), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC224108L2 | Lenti ORF clone of Human coagulation factor XII (Hageman factor) (F12), mGFP tagged |
CNY 6,460.00 |
|
| RC224108L3 | Lenti ORF clone of Human coagulation factor XII (Hageman factor) (F12), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC224108L4 | Lenti ORF clone of Human coagulation factor XII (Hageman factor) (F12), mGFP tagged |
CNY 6,460.00 |
|
| RG224108 | F12 (tGFP-tagged) - Human coagulation factor XII (Hageman factor) (F12) |
CNY 5,040.00 |
|
| SC126518 | F12 (untagged)-Human coagulation factor XII (Hageman factor) (F12) |
CNY 4,584.00 |
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