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POMT1 (NM_007171) Human Tagged ORF Clone
CAT＃: RC223823
- TrueORF®
POMT1 (Myc-DDK-tagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 1
ORF Plasmid: tGFP
"NM_007171" in other vectors (4)
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CNY 8,752.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LGMD2K; LGMDR11; MDDGA1; MDDGB1; MDDGC1; RT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223823 representing NM_007171
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGATTTTTGAAGCGCCCTGTAGTGGTGACGGCTGACATCAACTTGAGCCTTGTGGCCCTGACTG GGATGGGGTTACTGAGCCGGCTGTGGCGACTCACCTACCCGCGGGCTGTGGTTTTTGACGAAGTATATTA TGGGCAGTACATCTCTTTTTACATGAAACAAATCTTCTTCTTGGATGACAGTGGGCCGCCATTTGGCCAC ATGGTGCTGGCCTTGGGAGGTTATTTAGGAGGATTCGATGGCAATTTTTTGTGGAACAGAATTGGAGCAG AATACAGTAGCAACGTGCCTGTGTGGTCCCTGCGCCTGCTGCCAGCACTCGCGGGGGCCTTGTCGGTCCC CATGGCCTACCAGATAGTGTTGGAGCTCCACTTTTCTCATTGTGCCGCCATGGGAGCTGCTCTGTTGATG CTTATCGAGAATGCTCTCATCACTCAGTCAAGGCTAATGCTTTTGGAATCAGTGTTAATATTTTTCAATC TATTGGCCGTGTTGTCCTACCTGAAGTTCTTCAACTGCCAAAAGCACAGCCCTTTTTCTCTGAGCTGGTG GTTCTGGCTAACACTGACAGGGGTCGCTTGTTCCTGTGCAGTGGGCATCAAGTACATGGGTGTGTTCACG TACGTGCTCGTGCTGGGTGTTGCAGCTGTCCATGCCTGGCACCTGCTTGGAGACCAGACTTTGTCCAATG TAGGTGCTGATGTCCAGTGCTGCATGAGGCCGGCCTGTATGGGGCAGATGCGGATGTCACAGGGGGTCTG TGTGTTCTGTCACTTGCTCGCCCGAGCAGTGGCTTTGCTGGTCATCCCGGTCGTCCTGTACTTACTGTTC TTCTACGTCCACTTGATTCTAGTCTTCCGCTCTGGGCCCCACGACCAAATCATGTCCAGTGCCTTCCAGG CCAGCTTAGAGGGAGGACTAGCTCGGATCACCCAGGGTCAGCCACTGGAGGTGGCCTTTGGGTCCCAGGT CACTCTGAGGAACGTCTTTGGGAAACCTGTGCCCTGCTGGCTTCATTCCCACCAGGACACCTACCCCATG ATATATGAGAACGGCCGAGGCAGCTCCCACCAGCAACAGGTGACCTGTTACCCCTTCAAAGACGTCAATA ACTGGTGGATTGTAAAGGATCCCAGGAGGCACCAGCTGGTGGTGAGCAGCCCTCCGAGACCTGTGAGGCA CGGGGACATGGTGCAGCTGGTCCACGGCATGACCACCCGCTCCCTGAACACGCATGATGTTGCAGCCCCC CTGAGCCCCCATTCACAGGAGGTCTCCTGCTACATTGACTATAACATCTCCATGCCCGCCCAGAACCTCT GGAGACTGGAAATTGTGAACAGAGGATCTGACACAGACGTCTGGAAGACCATCCTCTCAGAGGTCCGCTT TGTGCACGTGAACACTTCCGCTGTCTTAAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTATCGGCAA CTGGAGATCGTCGGGGAGAAGCTGTCCCGGGGCTACCACGGGAGCACGGTGTGGAACGTGGAGGAGCACC GATACGGCGCGAGCCAGGAGCAGAGGGAGCGGGAACGGGAGCTGCACTCACCTGCGCAGGTGGACGTCAG CAGGAACCTCAGCTTCATGGCGAGATTCTCGGAGCTGCAGTGGAGGATGCTGGCGCTGAGAAGTGATGAC TCGGAACACAAGTACAGCTCCAGCCCACTGGAGTGGGTCACCCTGGACACCAATATTGCCTACTGGCTGC ACCCCAGGACCAGCGCTCAGATCCACCTACTTGGAAACATAGTGATCTGGGTTTCGGGCAGCCTCGCTCT GGCCATCTACGCCCTGCTGTCCTTGTGGTACCTGCTCCGACGGCGAAGAAATGTCCATGACCTCCCTCAG GATGCCTGGCTGCGCTGGGTGCTGGCTGGGGCGCTGTGTGCCGGTGGCTGGGCAGTGAACTACCTCCCGT TCTTCCTGATGGAGAAGACACTCTTCCTCTACCACTACCTGCCCGCACTCACCTTCCAAATCCTTCTGCT CCCTGTGGTCCTGCAGCACATCAGCGACCACCTGTGCAGGTCCCAGCTCCAGAGGAGCATCTTCAGCGCC CTGGTGGTGGCCTGGTACTCCTCCGCGTGCCACGTGTCCAACACGCTGCGCCCACTCACCTACGGGGACA AGTCACTCTCGCCACATGAACTCAAGGCCCTTCGCTGGAAAGACAGCTGGGACATCTTGATCCGAAAACA C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223823 representing NM_007171
Red=Cloning site Green=Tags(s) MWGFLKRPVVVTADINLSLVALTGMGLLSRLWRLTYPRAVVFDEVYYGQYISFYMKQIFFLDDSGPPFGH MVLALGGYLGGFDGNFLWNRIGAEYSSNVPVWSLRLLPALAGALSVPMAYQIVLELHFSHCAAMGAALLM LIENALITQSRLMLLESVLIFFNLLAVLSYLKFFNCQKHSPFSLSWWFWLTLTGVACSCAVGIKYMGVFT YVLVLGVAAVHAWHLLGDQTLSNVGADVQCCMRPACMGQMRMSQGVCVFCHLLARAVALLVIPVVLYLLF FYVHLILVFRSGPHDQIMSSAFQASLEGGLARITQGQPLEVAFGSQVTLRNVFGKPVPCWLHSHQDTYPM IYENGRGSSHQQQVTCYPFKDVNNWWIVKDPRRHQLVVSSPPRPVRHGDMVQLVHGMTTRSLNTHDVAAP LSPHSQEVSCYIDYNISMPAQNLWRLEIVNRGSDTDVWKTILSEVRFVHVNTSAVLKLSGAHLPDWGYRQ LEIVGEKLSRGYHGSTVWNVEEHRYGASQEQRERERELHSPAQVDVSRNLSFMARFSELQWRMLALRSDD SEHKYSSSPLEWVTLDTNIAYWLHPRTSAQIHLLGNIVIWVSGSLALAIYALLSLWYLLRRRRNVHDLPQ DAWLRWVLAGALCAGGWAVNYLPFFLMEKTLFLYHYLPALTFQILLLPVVLQHISDHLCRSQLQRSIFSA LVVAWYSSACHVSNTLRPLTYGDKSLSPHELKALRWKDSWDILIRKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007171 |
| ORF Size | 2241 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007171.3, NP_009102.3 |
| RefSeq Size | 3150 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 10585 |
| UniProt ID | Q9Y6A1 |
| Domains | PMT, MIR |
| Protein Families | Transmembrane |
| Protein Pathways | O-Mannosyl glycan biosynthesis |
| MW | 84.7 kDa |
| Gene Summary | The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223823L3 | Lenti-ORF clone of POMT1 (Myc-DDK-tagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 1 |
CNY 7,700.00 |
|
| RC223823L4 | Lenti-ORF clone of POMT1 (mGFP-tagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 1 |
CNY 7,700.00 |
|
| RG223823 | POMT1 (tGFP-tagged) - Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 1 |
CNY 6,370.00 |
|
| SC115661 | POMT1 (untagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 1 |
CNY 5,480.00 |
