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PORCN (NM_203475) Human Tagged ORF Clone
CAT＃: RC223764
PORCN (Myc-DDK-tagged)-Human porcupine homolog (Drosophila) (PORCN), transcript variant D
ORF Plasmid: tGFP
"NM_203475" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DHOF; FODH; MG61; PORC; PPN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223764 representing NM_203475
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTTTAGCCGCCAGGAATTTTTCCAGCAGCTACTGCAAGGCTGTCTCCTGCCTACTGCCCAGC AGGGCCTTGACCAGATCTGGCTGCTCCTTGCCATCTGCCTCGCCTGCCGCCTCCTCTGGAGGCTCGGGTT GCCATCCTACCTGAAGCATGCAAGCACCGTGGCAGGCGGGTTCTTCAGCCTCTACCACTTCTTCCAGCTG CACATGGTTTGGGTCGTGCTGCTCAGCCTCCTGTGCTACCTCGTGCTGTTCCTCTGCCGACATTCCTCCC ATCGAGGCGTCTTCCTATCCGTCACCATCCTCATCTACCTACTCATGGGTGAGATGCACATGGTAGACAC CGTGACATGGCACAAGATGCGAGGGGCACAGATGATTGTGGCCATGAAGGCAGTGTCTCTGGGCTTCGAC CTGGACCGGGGCGAGGTGGGTACGGTGCCCTCGCCAGTGGAGTTCATGGGCTACCTCTACTTCGTGGGCA CCATCGTCTTCGGGCCCTGGATATCCTTCCACAGCTACCTACAAGCTGTCCAAGGCCGCCCACTGAGCTG CCGGTGGCTGCAGAAGGTGGCCCGGAGCCTGGCACTGGCCCTGCTGTGCCTTGTGCTGTCCACTTGCGTG GGCCCCTACCTCTTCCCGTACTTCATCCCCCTCAACGGTGACCGCCTCCTTCGCAACAAGAAACGCAAAG CCAGGGGCACCATGGTAAGGTGGCTGCGAGCCTACGAGAGTGCTGTCTCCTTCCACTTCAGCAACTATTT TGTGGGCTTTCTTTCCGAGGCCACGGCCACGTTGGCGGGGGCTGGCTTTACCGAGGAGAAGGATCACCTG GAATGGGACCTGACGGTGTCCAAGCCACTGAATGTGGAGCTGCCTCGGTCAATGGTGGAAGTTGTCACAA GCTGGAACCTGCCCATGTCTTATTGGCTAAATAACTATGTTTTCAAGAATGCTCTCCGCCTGGGGACCTT CTCGGCTGTGCTGGTCACCTATGCAGCCAGCGCCCTCCTACATGGCTTCAGTTTCCACCTGGCTGCGGTC CTGCTGTCCCTGGCTTTTATCACTTACGTGGAGCATGTCCTCCGGAAGCGCCTGGCTCGGATCCTCAGTG CCTGTGTCTTGTCAAAGCGGTGCCCGCCAGACTGTTCGCACCAGCATCGCTTGGGCCTGGGGGTGCGAGC CTTAAACTTGCTCTTTGGAGCTCTGGCCATCTTCCACCTGGCCTACCTGGGCTCCCTGTTTGATGTCGAT GTGGATGACACCACAGAGGAGCAGGGCTACGGCATGGCATACACTGTCCACAAGTGGTCAGAGCTCAGCT GGGCCAGTCACTGGGTCACTTTTGGATGCTGGATCTTCTACCGTCTCATAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223764 representing NM_203475
Red=Cloning site Green=Tags(s) MATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQL HMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFD LDRGEVGTVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSCRWLQKVARSLALALLCLVLSTCV GPYLFPYFIPLNGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHL EWDLTVSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAV LLSLAFITYVEHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVD VDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203475 |
| ORF Size | 1383 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_203475.3 |
| RefSeq Size | 1761 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 64840 |
| UniProt ID | Q9H237 |
| Protein Families | Transmembrane |
| Protein Pathways | Wnt signaling pathway |
| MW | 52.1 kDa |
| Gene Summary | This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2013] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
EVI/WLS function is regulated by ubiquitylation and is linked to ER-associated degradation by ERLIN2
,null,
Journal of Cell Science
,PubMed ID 34406391
[PORCN]
|
|
ERAD-dependent control of the Wnt secretory factor Evi
,Glaeser, K;Urban, M;Fenech, E;Voloshanenko, O;Kranz, D;Lari, F;Christianson, JC;Boutros, M;,
EMBO J.
,PubMed ID 29378775
[PORCN]
|
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223764L1 | Lenti ORF clone of Human porcupine homolog (Drosophila) (PORCN), transcript variant D, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223764L2 | Lenti ORF clone of Human porcupine homolog (Drosophila) (PORCN), transcript variant D, mGFP tagged |
CNY 5,890.00 |
|
| RC223764L3 | Lenti ORF clone of Human porcupine homolog (Drosophila) (PORCN), transcript variant D, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223764L4 | Lenti ORF clone of Human porcupine homolog (Drosophila) (PORCN), transcript variant D, mGFP tagged |
CNY 5,890.00 |
|
| RG223764 | PORCN (tGFP-tagged) - Human porcupine homolog (Drosophila) (PORCN), transcript variant D |
CNY 4,370.00 |
|
| SC308204 | PORCN (untagged)-Human porcupine homolog (Drosophila) (PORCN), transcript variant D |
CNY 3,656.00 |
