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NEK11 (NM_145910) Human Tagged ORF Clone
CAT＃: RC223695
NEK11 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 2
ORF Plasmid: tGFP
"NM_145910" in other vectors (4)
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CNY 3,656.00
CNY 4,180.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223695 representing NM_145910
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAATTCCAAGAGGCAGCTAAGTGTGTGAGTGGATCAACAGCCATTTCCACTTATCCAAAGACCT TGATTGCAAGAAGATACGTGCTTCAACAAAAACTTGGCAGTGGAAGTTTTGGAACTGTCTATCTGGTTTC AGACAAGAAAGCCAAACGAGGAGAGGAATTAAAGGTACTTAAGGAAATATCTGTTGGAGAACTAAATCCA AATGAAACTGTACAGGCCAATTTGGAAGCCCAACTCCTCTCCAAGCTGGACCACCCAGCCATTGTCAAGT TCCATGCAAGTTTTGTGGAGCAAGATAATTTCTGCATTATCACGGAGTACTGTGAGGGCCGAGATCTGGA CGATAAAATTCAGGAATATAAACAAGCTGGAAAAATCTTTCCAGAAAATCAAATAATAGAATGGTTTATC CAGCTGCTGCTGGGAGTTGACTACATGCATGAGAGGAGGATACTTCATCGAGACTTAAAGTCAAAGAATG TATTTCTGAAAAATAATCTCCTTAAAATTGGAGATTTTGGAGTTTCTCGACTTCTAATGGGATCCTGTGA CCTGGCCACAACTTTAACTGGAACTCCCCATTATATGAGTCCTGAGGCTCTGAAACACCAAGGCTATGAC ACAAAGTCGGACATCTGGTCACTGGCATGCATTTTGTATGAGATGTGCTGCATGAATCATGCATTCGCTG GCTCCAATTTCTTATCCATTGTTTTAAAAATTGTTGAAGGTGACACACCTTCTCTCCCTGAGAGATATCC AAAAGAACTAAATGCCATCATGGAAAGCATGTTGAACAAGAATCCTTCATTAAGACCATCTGCTATCGAA ATTTTAAAAATCCCTTACCTTGATGAGCAGCTACAGAACCTAATGTGTAGATATTCAGAAATGACTCTGG AAGACAAAAATTTGGATTGTCAGAAGGAGGCTGCTCATATAATTAATGCCATGCAAAAAAGGATCCACCT GCAGACTCTGAGGGCACTGTCAGAAGTACAGAAAATGACGCCAAGAGAAAGGATGCGGCTGAGGAAGCTC CAGGCGGCTGATGAGAAAGCCAGGAAGCTGAAAAAGATTGTGGAAGAAAAATATGAAGAAAATAGCAAAC GAATGCAAGAATTGAGATCTCGGAACTTTCAGCAGCTGAGTGTTGATGTACTCCATGAAAAAACACATTT AAAAGGAATGGAAGAAAAGGAGGAGCAACCTGAGGGAAGACTTTCTTGTTCACCCCAGGACGAGGATGAA GAGAGGTGGCAAGGCAGGGAAGAGGAATCTGATGAACCAACTTTAGAGAACCTGCCTGAGTCTCAGCCTA TTCCTTCCATGGACCTCCACGAACTTGAATCAATTGTAGAGGATGCCACATCTGACCTTGGATACCATGC AACTCACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223695 representing NM_145910
Red=Cloning site Green=Tags(s) MLKFQEAAKCVSGSTAISTYPKTLIARRYVLQQKLGSGSFGTVYLVSDKKAKRGEELKVLKEISVGELNP NETVQANLEAQLLSKLDHPAIVKFHASFVEQDNFCIITEYCEGRDLDDKIQEYKQAGKIFPENQIIEWFI QLLLGVDYMHERRILHRDLKSKNVFLKNNLLKIGDFGVSRLLMGSCDLATTLTGTPHYMSPEALKHQGYD TKSDIWSLACILYEMCCMNHAFAGSNFLSIVLKIVEGDTPSLPERYPKELNAIMESMLNKNPSLRPSAIE ILKIPYLDEQLQNLMCRYSEMTLEDKNLDCQKEAAHIINAMQKRIHLQTLRALSEVQKMTPRERMRLRKL QAADEKARKLKKIVEEKYEENSKRMQELRSRNFQQLSVDVLHEKTHLKGMEEKEEQPEGRLSCSPQDEDE ERWQGREEESDEPTLENLPESQPIPSMDLHELESIVEDATSDLGYHATHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145910 |
| ORF Size | 1410 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145910.4 |
| RefSeq Size | 2080 bp |
| RefSeq ORF | 1413 bp |
| Locus ID | 79858 |
| UniProt ID | Q8NG66 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 53.8 kDa |
| Gene Summary | This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223695L3 | Lenti-ORF clone of NEK11 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 2 |
CNY 6,080.00 |
|
| RC223695L4 | Lenti-ORF clone of NEK11 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 2 |
CNY 6,080.00 |
|
| RG223695 | NEK11 (tGFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 2 |
CNY 4,560.00 |
|
| SC306293 | NEK11 (untagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 2 |
CNY 7,510.00 |
