TAF4 (NM_003185) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TAF(II)130; TAF(II)135; TAF2C; TAF2C1; TAF4A; TAFII-130; TAFII-135; TAFII130; TAFII135
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC223691 representing NM_003185 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGCTCGGATCTGCTGGACGAGGTCTTCTTCAACAGCGAGGTGGACGAGAAAGTGGTGAGCG ACCTGGTGGGCTCGCTGGAGTCGCAGCTGGCGGCCAGCGCGGCCCACCACCACCACCTCGCGCCGCGCAC GCCCGAGGTGCGGGCCGCGGCCGCCGGCGCGCTCGGGAACCATGTTGTGAGCGGCAGCCCGGCCGGAGCC GCGGGCGCAGGGCCGGCCGCCCCCGCCGAGGGCGCGCCCGGAGCGGCGCCGGAGCCGCCCCCCGCAGGTA GAGCGCGGCCGGGGGGCGGGGGGCCGCAGCGCCCGGGCCCCCCCTCACCGCGCCGCCCCCTTGTCCCCGC AGGGCCCGCGCCGCCCGCCGCGAAGCTGAGGCCGCCGCCCGAGGGCAGCGCGGGGTCCTGCGCCCCGGTG CCCGCCGCCGCCGCCGTCGCCGCGGGGCCCGAGCCCGCCCCCGCCGGCCCCGCCAAGCCCGCCGGCCCCG CCGCGCTGGCCGCCCGCGCCGGCCCCGGCCCCGGGCCCGGCCCCGGCCCCGGCCCCGGCCCTGGCCCTGG CAAGCCCGCCGGCCCCGGCGCCGCGCAAACTTTGAATGGGAGCGCCGCGCTGCTGAACTCGCACCACGCC GCCGCACCTGCTGTCAGCCTGGTCAACAACGGGCCCGCCGCGCTGCTGCCGCTGCCCAAGCCCGCCGCCC CCGGCACTGTCATCCAGACGCCCCCCTTCGTGGGCGCCGCCGCGCCCCCCGCGCCCGCCGCGCCCTCGCC CCCCGCCGCCCCCGCGCCCGCCGCCCCCGCCGCCGCCCCGCCCCCGCCACCCCCCGCGCCCGCCACTCTG GCCCGGCCGCCCGGCCACCCCGCCGGACCCCCGACCGCCGCGCCCGCCGTGCCGCCCCCCGCCGCCGCCC AGAACGGGGGCAGCGCCGGGGCAGCCCCCGCCCCCGCCCCGGCCGCCGGGGGCCCCGCGGGGGTCAGCGG CCAACCCGGGCCCGGCGCGGCGGCTGCGGCGCCGGCGCCGGGGGTCAAGGCCGAGTCGCCCAAGAGGGTG GTGCAGGCGGCGCCCCCGGCGGCGCAGACCCTGGCGGCCAGCGGCCCGGCCAGCACGGCGGCCAGCATGG TCATCGGGCCAACTATGCAAGGGGCGCTGCCCAGCCCGGCCGCCGTCCCGCCGCCCGCCCCCGGGACCCC CACCGGGCTGCCCAAAGGCGCGGCCGGCGCAGTGACCCAGAGCCTGTCCCGGACGCCCACGGCCACCACC AGCGGGATTCGGGCCACCCTGACGCCCACCGTGCTGGCCCCCCGCTTGCCGCAGCCGCCTCAGAACCCGA CCAACATCCAGAACTTCCAGCTGCCCCCAGGAATGGTCCTCGTCCGAAGTGAGAATGGGCAGTTGTTAAT GATTCCTCAGCAGGCCTTGGCCCAGATGCAGGCGCAGGCCCATGCCCAGCCTCAGACCACCATGGCGCCT CGCCCTGCCACCCCCACAAGTGCCCCTCCCGTCCAGATCTCCACCGTACAGGCACCTGGAACACCTATCA TTGCACGGCAGGTGACCCCAACTACCATAATTAAGCAAGTGTCTCAGGCCCAGACAACGGTGCAGCCCAG TGCAACCCTGCAGCGCTCGCCCGGCGTCCAGCCTCAGCTCGTTCTGGGTGGCGCTGCCCAGACGGCTTCA CTTGGGACGGCGACGGCTGTTCAGACGGGGACTCCTCAGCGCACGGTACCAGGGGCGACCACCACTTCCT CAGCTGCCACGGAAACTATGGAAAACGTGAAGAAATGTAAAAATTTCCTATCTACGTTAATAAAACTGGC TTCATCTGGCAAGCAGTCTACAGAGACAGCAGCTAATGTGAAAGAGCTCGTGCAGAATTTACTGGATGGA AAAATAGAAGCAGAAGATTTCACAAGCAGGTTATACCGAGAACTTAATTCTTCACCTCAACCTTACCTTG TGCCTTTCCTGAAGAGGAGCTTACCCGCCTTGAGACAGCTGACCCCCGACTCCGCGGCCTTCATCCAGCA GAGCCAGCAGCAGCCGCCACCGCCCACCTCGCAGGCCACCACTGCGCTCACGGCCGTGGTGCTGAGTAGC TCGGTCCAGCGCACGGCCGGGAAGACGGCGGCCACCGTGACCAGTGCCCTCCAGCCCCCTGTGCTCAGCC TCACGCAGCCCACGCAGGTCGGCGTCGGCAAGCAGGGGCAACCCACACCGCTGGTCATCCAGCAGCCTCC GAAGCCAGGAGCCCTGATCCGGCCCCCGCAGGTGACGTTGACGCAGACACCCATGGTCGCCCTGCGGCAG CCTCACAACCGGATCATGCTCACCACGCCTCAGCAGATCCAGCTGAACCCACTGCAGCCAGTCCCTGTGG TGAAACCCGCCGTGTTACCTGGAACCAAAGCCCTTTCTGCTGTCTCGGCACAAGCAGCTGCTGCACAGAA AAATAAACTCAAGGAGCCTGGGGGAGGTTCGTTTCGGGACGATGATGACATTAATGATGTTGCATCGATG GCTGGAGTAAACTTGTCAGAAGAAAGTGCAAGAATATTAGCCACGAACTCTGAATTGGTGGGCACGCTAA CGCGGTCCTGTAAAGATGAAACCTTCCTCCTCCAAGCGCCTTTGCAGAGAAGAATATTAGAAATAGGTAA AAAACATGGTATAACGGAATTACATCCAGATGTAGTAAGTTATGTATCACATGCCACGCAACAAAGGCTA CAGAATCTTGTAGAGAAAATATCAGAAACAGCTCAGCAGAAGAACTTTTCTTACAAGGATGACGACAGAT ATGAGCAGGCGAGTGACGTCCGGGCACAGCTCAAGTTTTTTGAACAGCTTGATCAAATCGAAAAGCAGAG GAAGGATGAGCAGGAGCGGGAGATCCTGATGAGGGCAGCAAAGTCTCGGTCAAGACAAGAAGATCCAGAA CAGTTAAGGCTGAAACAGAAGGCAAAGGAGATGCAGCAACAGGAACTGGCACAAATGAGACAGCGGGACG CCAACCTCACAGCACTAGCAGCGATCGGGCCCAGGAAAAAGAGGAAAGTGGACTGTCCGGGGCCGGGCTC AGGAGCAGAGGGGTCGGGCCCCGGCTCAGTGGTCCCAGGCAGCTCGGGTGTCGGAACCCCCAGACAGTTC ACGCGACAAAGAATCACGCGGGTCAACCTCAGGGACCTCATATTTTGTTTAGAAAATGAACGTGAGACAA GCCATTCACTGCTGCTCTACAAAGCATTCCTTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC223691 representing NM_003185 Red=Cloning site Green=Tags(s) MAAGSDLLDEVFFNSEVDEKVVSDLVGSLESQLAASAAHHHHLAPRTPEVRAAAAGALGNHVVSGSPAGA AGAGPAAPAEGAPGAAPEPPPAGRARPGGGGPQRPGPPSPRRPLVPAGPAPPAAKLRPPPEGSAGSCAPV PAAAAVAAGPEPAPAGPAKPAGPAALAARAGPGPGPGPGPGPGPGPGKPAGPGAAQTLNGSAALLNSHHA AAPAVSLVNNGPAALLPLPKPAAPGTVIQTPPFVGAAAPPAPAAPSPPAAPAPAAPAAAPPPPPPAPATL ARPPGHPAGPPTAAPAVPPPAAAQNGGSAGAAPAPAPAAGGPAGVSGQPGPGAAAAAPAPGVKAESPKRV VQAAPPAAQTLAASGPASTAASMVIGPTMQGALPSPAAVPPPAPGTPTGLPKGAAGAVTQSLSRTPTATT SGIRATLTPTVLAPRLPQPPQNPTNIQNFQLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAP RPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTAS LGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCKNFLSTLIKLASSGKQSTETAANVKELVQNLLDG KIEAEDFTSRLYRELNSSPQPYLVPFLKRSLPALRQLTPDSAAFIQQSQQQPPPPTSQATTALTAVVLSS SVQRTAGKTAATVTSALQPPVLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQ PHNRIMLTTPQQIQLNPLQPVPVVKPAVLPGTKALSAVSAQAAAAQKNKLKEPGGGSFRDDDDINDVASM AGVNLSEESARILATNSELVGTLTRSCKDETFLLQAPLQRRILEIGKKHGITELHPDVVSYVSHATQQRL QNLVEKISETAQQKNFSYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDEQEREILMRAAKSRSRQEDPE QLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRKKRKVDCPGPGSGAEGSGPGSVVPGSSGVGTPRQF TRQRITRVNLRDLIFCLENERETSHSLLLYKAFLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_003185
ORF Size	3255 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003185.3, NP_003176.2
RefSeq Size	4647 bp
RefSeq ORF	3258 bp
Locus ID	6874
UniProt ID	O00268
Protein Pathways	Basal transcription factors, Huntington's disease
MW	109.9 kDa
Gene Summary	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq, Jul 2008]

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