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Eph receptor A5 (EPHA5) (NM_182472) Human Tagged ORF Clone
CAT＃: RC223526
- TrueORF®
EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 2
ORF Plasmid: tGFP
"NM_182472" in other vectors (4)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CEK7; EHK-1; EHK1; EK7; HEK7; TYRO4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223526 representing NM_182472
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCTCGGGGCCCCGGGGTGCGGGACGCCGGCGGCCCCCAAGCGGCGGCGGCGACACCCCCATCA CCCCAGCGTCCCTGGCCGGCTGCTACTCTGCACCTCGACGGGCTCCCCTCTGGACGTGCCTTCTCCTGTG CGCCGCACTCCGGACCCTCCTGGCCAGCCCCAGCAACGAAGTGAATTTATTGGATTCACGCACTGTCATG GGGGACCTGGGATGGATTGCTTTTCCAAAAAATGGGTGGGAAGAGATTGGTGAAGTGGATGAAAATTATG CCCCTATCCACACATACCAAGTATGCAAAGTGATGGAACAGAATCAGAATAACTGGCTTTTGACCAGTTG GATCTCCAATGAAGGTGCTTCCAGAATCTTCATAGAACTCAAATTTACCCTGCGGGACTGCAACAGCCTT CCTGGAGGACTGGGGACCTGTAAGGAAACCTTTAATATGTATTACTTTGAGTCAGATGATCAGAATGGGA GAAACATCAAGGAAAACCAATACATCAAAATTGATACCATTGCTGCCGATGAAAGCTTTACAGAACTTGA TCTTGGTGACCGTGTTATGAAACTGAATACAGAGGTCAGAGATGTAGGACCTCTAAGCAAAAAGGGATTT TATCTTGCTTTTCAAGATGTTGGTGCTTGCATTGCTCTGGTTTCTGTGCGTGTATACTATAAAAAATGCC CTTCTGTGGTACGACACTTGGCTGTCTTCCCTGACACCATCACTGGAGCTGATTCTTCCCAATTGCTCGA AGTGTCAGGCTCCTGTGTCAACCATTCTGTGACCGATGAACCTCCCAAAATGCACTGCAGCGCCGAAGGG GAGTGGCTGGTGCCCATCGGGAAATGCATGTGCAAGGCAGGATATGAAGAGAAAAATGGCACCTGTCAAG TGTGCAGACCTGGGTTCTTCAAAGCCTCACCTCACATCCAGAGCTGCGGCAAATGTCCACCTCACAGTTA TACCCATGAGGAAGCTTCAACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGAGAGTCTGATCCACCC ACAATGGCATGCACAAGACCCCCCTCTGCTCCTCGGAATGCCATCTCAAATGTTAATGAAACTAGTGTCT TTCTGGAATGGATTCCGCCTGCTGACACTGGTGGAAGGAAAGACGTGTCATATTATATTGCATGCAAGAA GTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTACCTTCCCCGGCAAAGCGGC CTGAAAAACACCTCTGTCATGATGGTGGATCTACTCGCTCACACAAACTATACCTTTGAGATTGAGGCAG TGAATGGAGTGTCCGACTTGAGCCCAGGAGCCCGGCAGTATGTGTCTGTAAATGTAACCACAAATCAAGC AGCTCCATCTCCAGTCACCAATGTGAAAAAAGGGAAAATTGCAAAAAACAGCATCTCTTTGTCTTGGCAA GAACCAGATCGTCCCAATGGAATCATCCTAGAGTATGAAATCAAGTATTTTGAAAAGGACCAAGAGACCA GCTACACGATTATCAAATCTAAAGAGACAACTATTACTGCAGAGGGCTTGAAACCAGCTTCAGTTTATGT CTTCCAAATTCGAGCACGTACAGCAGCAGGCTATGGTGTCTTCAGTCGAAGATTTGAGTTTGAAACCACC CCAGTGTTTGCAGCATCCAGCGATCAAAGCCAGATTCCTGTAATTGCTGTGTCTGTGACAGTGGGAGTCA TTTTGTTGGCAGTGGTTATCGGCGTCCTCCTCAGTGGAAGGCGGTGTGGCTACAGCAAAGCAAAACAAGA TCCAGAAGAGGAAAAGATGCATTTTCATAATGGGCACATTAAACTGCCAGGAGTAAGAACTTACATTGAT CCACATACCTATGAGGATCCCAATCAAGCTGTCCACGAATTTGCTAAGGAGATAGAAGCATCATGTATCA CCATTGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGTAGTGGACGTTTGAAACTACCAGGAAA AAGAGAATTACCTGTGGCTATCAAAACCCTTAAAGTAGGCTATACTGAAAAGCAACGCAGAGATTTCCTA GGTGAAGCAAGTATCATGGGACAGTTTGATCATCCTAACATCATCCATTTAGAAGGTGTGGTGACCAAAA GTAAACCAGTGATGATCGTGACAGAGTATATGGAGAATGGCTCTTTAGATACATTTTTGAAGAAAAACGA TGGGCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGTATCTCTGCAGGAATGAAGTACCTTTCT GACATGGGCTATGTGCATAGAGATCTTGCTGCCAGAAACATCTTAATCAACAGTAACCTTGTGTGCAAAG TGTCTGACTTTGGACTTTCCCGGGTACTGGAAGATGATCCCGAGGCAGCCTACACCACAAGGGGAGGAAA AATTCCAATCAGATGGACTGCCCCAGAAGCAATAGCTTTCCGAAAGTTTACTTCTGCCAGTGATGTCTGG AGTTATGGAATAGTAATGTGGGAAGTTGTGTCTTATGGAGAGAGACCCTACTGGGAGATGACCAATCAAG ATGTGATTAAAGCGGTAGAGGAAGGCTATCGTCTGCCAAGCCCCATGGATTGTCCTGCTGCTCTCTATCA GTTAATGCTGGATTGCTGGCAGAAAGAGCGAAATAGCAGGCCCAAGTTTGATGAAATAGTCAACATGTTG GACAAGCTGATACGTAACCCAAGTAGTCTGAAGACGCTGGTTAATGCATCCTGCAGAGTATCTAATTTAT TGGCAGAACATAGCCCACTAGGATCTGGGGCCTACAGATCAGTAGGTGAATGGCTAGAGGCAATCAAGAT GGGCCGGTATACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGACCTTG GAGGATTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAA TGAAGGTGCAGCTGGTAAACGGAATGGTGCCATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223526 representing NM_182472
Red=Cloning site Green=Tags(s) MRGSGPRGAGRRRPPSGGGDTPITPASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVM GDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSL PGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGF YLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEG EWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPP TMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSG LKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQ EPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETT PVFAASSDQSQIPVIAVSVTVGVILLAVVIGVLLSGRRCGYSKAKQDPEEEKMHFHNGHIKLPGVRTYID PHTYEDPNQAVHEFAKEIEASCITIERVIGAGEFGEVCSGRLKLPGKRELPVAIKTLKVGYTEKQRRDFL GEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENGSLDTFLKKNDGQFTVIQLVGMLRGISAGMKYLS DMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAFRKFTSASDVW SYGIVMWEVVSYGERPYWEMTNQDVIKAVEEGYRLPSPMDCPAALYQLMLDCWQKERNSRPKFDEIVNML DKLIRNPSSLKTLVNASCRVSNLLAEHSPLGSGAYRSVGEWLEAIKMGRYTEIFMENGYSSMDAVAQVTL EDLRRLGVTLVGHQKKIMNSLQEMKVQLVNGMVPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182472 |
| ORF Size | 3045 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182472.5 |
| RefSeq Size | 7792 bp |
| RefSeq ORF | 3048 bp |
| Locus ID | 2044 |
| UniProt ID | P54756 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 112.6 kDa |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223526L3 | Lenti-ORF clone of EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 2 |
CNY 12,070.00 |
|
| RC223526L4 | Lenti-ORF clone of EPHA5 (mGFP-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 2 |
CNY 12,070.00 |
|
| RG223526 | EPHA5 (tGFP-tagged) - Human EPH receptor A5 (EPHA5), transcript variant 2 |
CNY 11,210.00 |
|
| SC310005 | EPHA5 (untagged)-Human EPH receptor A5 (EPHA5), transcript variant 2 |
CNY 10,450.00 |
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