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Synaptojanin (SYNJ1) (NM_003895) Human Tagged ORF Clone
CAT＃: RC223432
- TrueORF®
SYNJ1 (Myc-DDK-tagged)-Human synaptojanin 1 (SYNJ1), transcript variant 1
ORF Plasmid: tGFP
"NM_003895" in other vectors (5)
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CNY 16,060.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DEE53; EIEE53; INPP5G; PARK20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223432 representing NM_003895
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAAGAGATGGGCCTGCTGGAGCGGAAGTGACGCTCCCGGCGGCTGTGGCGGCGGCTGCGGGAGAA GGAGGAGGAGGAGCCGGAGGAAGAGGGCTGCCTCCGAAGAAAGGAGAATGGCGTTCAGTAAAGGATTCCG GATCTATCACAAATTGGATCCCCCACCTTTCAGCCTCATAGTGGAAACTAGGCATAAGGAAGAATGTCTC ATGTTCGAGTCTGGGGCTGTCGCTGTGCTCTCATCTGCAGAAAAAGAGGCAATCAAGGGTACATACTCCA AAGTACTGGATGCATATGGACTCTTAGGTGTTCTGCGGTTAAATCTTGGTGATACTATGTTACATTATCT GGTCCTAGTCACTGGATGTATGTCTGTTGGAAAAATTCAAGAATCTGAAGTTTTCCGAGTTACTTCCACT GAGTTTATATCACTGCGAATCGATTCTTCAGATGAGGATCGCATTTCAGAAGTGCGGAAAGTTTTGAATT CAGGAAACTTTTATTTTGCATGGTCTGCATCTGGCATCAGTTTAGATTTGAGTCTTAATGCGCATCGTAG CATGCAAGAACAGACAACTGATAATAGATTTTTCTGGAATCAGTCTTTGCATTTGCATCTCAAACACTAT GGCGTGAATTGTGATGACTGGTTATTACGTCTTATGTGTGGAGGAGTAGAAATCAGAACAATTTATGCTG CTCATAAACAGGCGAAGGCTTGCCTCATTTCAAGATTAAGCTGTGAACGAGCTGGGACCAGGTTTAATGT CCGGGGAACAAATGATGATGGTCATGTTGCCAATTTTGTAGAAACAGAACAGGTTGTGTACTTAGATGAC TCAGTTTCTTCCTTCATACAAATCCGAGGATCTGTTCCATTGTTCTGGGAGCAACCAGGGTTGCAAGTGG GATCTCATCGTGTCCGTATGTCAAGGGGATTTGAAGCCAATGCACCTGCTTTTGACAGGCATTTTAGAAC ACTTAAGAACTTATATGGTAAACAAATAATAGTAAATTTGCTTGGATCTAAGGAAGGTGAACATATGCTA AGTAAAGCTTTCCAGAGTCATTTGAAAGCTTCTGAACATGCTGCTGATATCCAGATGGTGAATTTTGACT ATCATCAAATGGTTAAGGGAGGAAAGGCAGAAAAATTACATAGTGTTCTTAAACCTCAAGTCCAGAAGTT TCTAGATTATGGATTTTTTTATTTCAATGGAAGTGAAGTTCAAAGATGCCAGAGTGGTACAGTTCGAACA AACTGCTTGGATTGTCTTGATAGAACAAATAGTGTGCAGGCATTTCTTGGCTTAGAGATGCTAGCTAAAC AGTTGGAAGCTCTTGGTTTAGCTGAAAAGCCTCAGTTGGTGACTCGCTTTCAAGAAGTTTTTCGGTCAAT GTGGTCCGTGAATGGTGATTCAATCAGTAAGATATATGCAGGAACTGGAGCTCTTGAAGGGAAAGCGAAG TTAAAAGATGGTGCTCGCTCTGTTACCCGAACAATTCAGAATAACTTCTTTGACAGCTCCAAGCAAGAGG CCATTGATGTTTTGCTACTGGGAAATACTCTGAATAGTGATTTAGCTGACAAAGCTCGAGCACTTTTAAC TACTGGAAGTTTGCGTGTTTCTGAGCAGACATTACAGTCAGCATCTTCTAAAGTACTAAAGAGCATGTGT GAGAATTTCTACAAATATTCAAAGCCTAAGAAAATTCGAGTATGTGTCGGAACCTGGAATGTGAATGGTG GGAAGCAATTTCGCAGCATAGCTTTTAAGAATCAGACACTCACTGACTGGCTTCTTGATGCACCCAAGTT AGCTGGCATCCAGGAGTTTCAAGATAAAAGAAGTAAGCCAACTGATATATTTGCAATTGGTTTTGAAGAA ATGGTAGAATTGAATGCTGGAAACATTGTGAGTGCAAGCACAACAAATCAGAAGCTCTGGGCTGTAGAAC TTCAGAAGACAATCTCCAGAGACAACAAGTATGTGCTGCTGGCTTCTGAACAGTTGGTGGGCGTCTGTTT GTTTGTTTTTATCAGACCACAGCATGCTCCTTTTATCAGGGATGTTGCAGTTGATACTGTGAAGACTGGA ATGGGAGGTGCAACTGGAAATAAGGGAGCAGTTGCAATCCGAATGCTCTTCCATACAACCAGCCTTTGCT TCGTCTGTAGCCACTTTGCTGCAGGGCAGTCACAAGTCAAAGAAAGAAATGAAGATTTTATAGAAATAGC ACGAAAATTGAGTTTTCCTATGGGAAGGATGCTATTTTCCCATGACTATGTATTTTGGTGTGGTGATTTC AACTATCGAATCGATCTCCCTAACGAAGAAGTTAAAGAGCTCATAAGACAGCAAAATTGGGATTCTCTTA TAGCAGGAGATCAACTTATCAATCAGAAAAATGCTGGACAGGTTTTTAGAGGATTTTTAGAAGGAAAGGT AACCTTTGCTCCGACATATAAGTATGACTTGTTTTCTGACGACTATGACACCAGTGAAAAGTGCCGCACC CCTGCCTGGACAGACCGTGTCCTTTGGAGAAGGAGGAAATGGCCTTTTGATAGATCAGCTGAAGATCTAG ATCTTCTAAATGCTAGTTTTCAAGATGAAAGCAAAATTCTGTACACGTGGACTCCAGGCACTTTGCTGCA CTATGGAAGAGCTGAGCTGAAGACTTCTGACCACAGGCCTGTCGTTGCCCTGATTGATATAGATATATTT GAAGTTGAAGCTGAAGAGAGGCAAAACATTTATAAAGAAGTAATTGCAGTTCAGGGTCCACCAGATGGTA CAGTATTGGTCTCAATCAAAAGTTCTTTACCAGAAAATAATTTTTTTGATGATGCCTTGATTGATGAGCT TCTGCAGCAGTTTGCAAGTTTTGGTGAAGTTATACTTATAAGATTTGTAGAAGATAAAATGTGGGTTACA TTTTTGGAGGGAAGCTCTGCCTTGAATGTTCTGAGCCTAAATGGTAAAGAGTTATTGAATCGGACTATAA CTATTGCTTTAAAAAGTCCAGACTGGATCAAAAATTTGGAAGAAGAAATGAGTTTAGAGAAAATTAGCAT TGCATTGCCATCATCAACAAGCTCTACCCTGCTTGGTGAAGATGCAGAGGTTGCAGCAGATTTTGATATG GAAGGTGATGTTGATGACTATAGTGCTGAAGTGGAGGAACTTCTTCCTCAGCATCTCCAGCCATCTTCAA GTTCCGGCCTTGGTACTTCCCCCAGCTCTTCACCCCGAACTAGTCCCTGCCAGTCACCTACAATATCAGA GGGTCCTGTACCTTCCCTTCCCATCAGACCAAGCCGAGCACCGTCAAGAACTCCTGGGCCTCCCAGTGCA CAGAGTTCTCCTATTGACGCGCAGCCAGCAACGCCGCTGCCGCAGAAAGACCCCGCCCAGCCCTTGGAGC CCAAGCGGCCGCCGCCGCCCCGCCCGGTCGCCCCTCCCACACGCCCGGCTCCCCCACAGAGACCTCCTCC GCCTTCAGGGGCTAGGAGTCCTGCACCCACTAGAAAGGAATTTGGAGGTATTGGAGCCCCTCCCAGTCCT GGGGTAGCTAGGAGAGAGATGGAAGCACCCAAAAGCCCTGGAACAACAAGGAAAGATAATATAGGACGCA GTCAGCCTTCACCTCAAGCAGGACTTGCAGGCCCAGGACCTGCTGGATACAGTACAGCCAGACCGACGAT TCCTCCTCGTGCTGGAGTTATCAGTGCCCCACAGAGCCACGCGCGGGCATCTGCTGGAAGACTGACTCCT GAAAGCCAAAGCAAAACATCAGAAACGTCGAAAGGTTCAACTTTCCTTCCTGAACCACTGAAGCCTCAGG CTGCTTTTCCTCCGCAGTCTTCTTTGCCCCCGCCTGCTCAAAGGTTGCAAGAGCCTCTTGTCCCTGTGGC AGCACCTATGCCTCAGTCTGGCCCCCAGCCAAATTTGGAAACCCCACCACAACCACCACCTCGAAGCAGG TCATCCCATAGCTTGCCTTCAGAAGCTTCCTCACAACCGCAAGTAAAAACAAATGGAATCTCTGATGGCA AAAGAGAATCACCATTAAAGATTGACCCATTTGAAGATCTGTCATTTAATCTGCTTGCTGTATCAAAGGC TCAGCTATCTGTTCAAACGTCACCTGTTCCCACCCCAGACCCAAAGAGGTTGATTCAGTTGCCTTCTGCA ACGCAAAGTAATGTTTTGAGTTCTGTAAGTTGCATGCCAACAATGCCTCCAATTCCAGCTCGGAGTCAAT CCCAGGAAAATATGCGAAGTTCTCCAAACCCATTTATTACTGGCTTGACCAGGACAAATCCTTTCAGTGA CAGGACTGCTGCTCCTGGAAACCCATTTAGAGCCAAGTCTGAAGAATCAGAGGCAACTTCATGGTTCTCC AAAGAAGAGCCCGTTACTATCAGTCCTTTCCCTTCTCTGCAGCCTCTTGGTCATAACAAAAGCAGGGCTT CATCTTCACTTGATGGCTTTAAGGACAGTTTTGATCTACAGGGCCAGTCTACATTAAAAATTAGCAACCC GAAAGGATGGGTAACCTTCGAGGAAGAAGAGGATTTTGGTGTGAAAGGGAAGTCAAAGTCAGCTTGTTCA GACTTACTGGGTAATCAGCCAAGTTCATTTTCTGGCTCCAACCTGACATTGAATGATGACTGGAATAAAG GTACAAATGTCTCCTTCTGTGTGTTGCCGTCAAGAAGACCTCCTCCACCTCCTGTCCCTCTGCTCCCGCC CGGCACCAGCCCTCCAGTAGATCCTTTCACGACCTTGGCCTCTAAGGCTTCACCCACACTGGACTTTACA GAAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223432 representing NM_003895
Red=Cloning site Green=Tags(s) MRKRWACWSGSDAPGGCGGGCGRRRRRSRRKRAASEERRMAFSKGFRIYHKLDPPPFSLIVETRHKEECL MFESGAVAVLSSAEKEAIKGTYSKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTST EFISLRIDSSDEDRISEVRKVLNSGNFYFAWSASGISLDLSLNAHRSMQEQTTDNRFFWNQSLHLHLKHY GVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYLDD SVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKNLYGKQIIVNLLGSKEGEHML SKAFQSHLKASEHAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFNGSEVQRCQSGTVRT NCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAK LKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKSMC ENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEE MVELNAGNIVSASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTG MGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFIEIARKLSFPMGRMLFSHDYVFWCGDF NYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQVFRGFLEGKVTFAPTYKYDLFSDDYDTSEKCRT PAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIF EVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQQFASFGEVILIRFVEDKMWVT FLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDM EGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSA QSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRPAPPQRPPPPSGARSPAPTRKEFGGIGAPPSP GVARREMEAPKSPGTTRKDNIGRSQPSPQAGLAGPGPAGYSTARPTIPPRAGVISAPQSHARASAGRLTP ESQSKTSETSKGSTFLPEPLKPQAAFPPQSSLPPPAQRLQEPLVPVAAPMPQSGPQPNLETPPQPPPRSR SSHSLPSEASSQPQVKTNGISDGKRESPLKIDPFEDLSFNLLAVSKAQLSVQTSPVPTPDPKRLIQLPSA TQSNVLSSVSCMPTMPPIPARSQSQENMRSSPNPFITGLTRTNPFSDRTAAPGNPFRAKSEESEATSWFS KEEPVTISPFPSLQPLGHNKSRASSSLDGFKDSFDLQGQSTLKISNPKGWVTFEEEEDFGVKGKSKSACS DLLGNQPSSFSGSNLTLNDDWNKGTNVSFCVLPSRRPPPPPVPLLPPGTSPPVDPFTTLASKASPTLDFT ER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003895 |
| ORF Size | 4836 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003895.3, NP_003886.3 |
| RefSeq Size | 7075 bp |
| RefSeq ORF | 4839 bp |
| Locus ID | 8867 |
| UniProt ID | O43426 |
| Domains | IPPc, Syja_N, Exo_endo_phos |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 177.5 kDa |
| Gene Summary | This gene encodes a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223432L3 | Lenti-ORF clone of SYNJ1 (Myc-DDK-tagged)-Human synaptojanin 1 (SYNJ1), transcript variant 1 |
CNY 17,960.00 |
|
| RC223432L4 | Lenti-ORF clone of SYNJ1 (mGFP-tagged)-Human synaptojanin 1 (SYNJ1), transcript variant 1 |
CNY 17,960.00 |
|
| RG223432 | SYNJ1 (tGFP-tagged) - Human synaptojanin 1 (SYNJ1), transcript variant 1 |
CNY 17,670.00 |
|
| SC309424 | SYNJ1 (untagged)-Human synaptojanin 1 (SYNJ1), transcript variant 1 |
CNY 31,070.00 |
|
| SC328081 | SYNJ1 (untagged)-Human synaptojanin 1 (SYNJ1) transcript variant 1 |
CNY 31,070.00 |
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