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SERCA3 (ATP2A3) (NM_174956) Human Tagged ORF Clone
CAT＃: RC222876
- TrueORF®
ATP2A3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 3
ORF Plasmid: tGFP
"NM_174956" in other vectors (4)
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CNY 10,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SERCA3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222876 representing NM_174956
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGGCGCATCTGCTCCCGGCCGCCGACGTGCTGCGCCACTTCTCGGTGACAGCCGAGGGCGGCC TGAGCCCGGCGCAGGTGACCGGCGCGCGGGAGCGCTACGGCCCCAACGAGCTCCCGAGTGAGGAAGGGAA GTCCCTGTGGGAGCTGGTGCTGGAACAGTTTGAGGACCTCCTGGTGCGCATCCTGCTGCTGGCTGCCCTT GTCTCCTTTGTCCTGGCCTGGTTCGAGGAGGGCGAGGAGACCACGACCGCCTTCGTGGAGCCCCTGGTCA TCATGCTGATCCTCGTGGCCAACGCCATTGTGGGCGTGTGGCAGGAACGCAACGCCGAGAGTGCCATCGA GGCCCTGAAGGAGTATGAGCCTGAGATGGGCAAGGTGATCCGCTCGGACCGCAAGGGCGTGCAGAGGATC CGTGCCCGGGACATCGTCCCAGGGGACATTGTAGAAGTGGCAGTGGGGGACAAAGTGCCTGCTGACCTCC GCCTCATCGAGATCAAGTCCACCACGCTGCGAGTGGACCAGTCCATCCTGACGGGTGAATCTGTGTCCGT GACCAAGCACACAGAGGCCATCCCAGACCCCAGAGCTGTGAACCAGGACAAGAAGAACATGCTGTTTTCT GGCACCAATATCACATCGGGCAAAGCGGTGGGTGTGGCCGTGGCCACCGGCCTGCACACGGAGCTGGGCA AGATCCGGAGCCAGATGGCGGCAGTCGAGCCCGAGCGGACGCCGCTGCAGCGCAAGCTGGACGAGTTTGG ACGGCAGCTGTCCCACGCCATCTCTGTGATCTGCGTGGCCGTGTGGGTCATCAACATCGGCCACTTCGCC GACCCGGCCCACGGTGGCTCCTGGCTGCGTGGCGCTGTCTACTACTTCAAGATCGCCGTGGCCCTGGCGG TGGCGGCCATCCCCGAGGGCCTCCCGGCTGTCATCACTACATGCCTGGCACTGGGCACGCGGCGCATGGC ACGCAAGAACGCCATCGTGCGAAGCCTGCCGTCCGTGGAGACCCTGGGCTGCACCTCAGTCATCTGCTCC GACAAGACGGGCACGCTCACCACCAATCAGATGTCTGTCTGCCGGATGTTCGTGGTAGCCGAGGCCGATG CGGGCTCCTGCCTTTTGCACGAGTTCACCATCTCGGGTACCACGTATACCCCCGAGGGCGAAGTGCGGCA GGGGGATCAGCCTGTGCGCTGCGGCCAGTTCGACGGGCTGGTGGAGCTGGCGACCATCTGCGCCCTGTGC AACGACTCGGCTCTGGACTACAACGAGGCCAAGGGTGTGTATGAGAAGGTGGGAGAGGCCACGGAGACAG CTCTGACTTGCCTGGTGGAGAAGATGAACGTGTTCGACACCGACCTGCAGGCTCTGTCCCGGGTGGAGCG AGCTGGCGCCTGTAACACGGTCATCAAGCAGCTGATGCGGAAGGAGTTCACCCTGGAGTTCTCCCGAGAC CGGAAATCCATGTCCGTGTACTGCACGCCCACCCGCCCTCACCCTACTGGCCAGGGCAGCAAGATGTTTG TGAAGGGGGCTCCTGAGAGTGTGATCGAGCGCTGTAGCTCAGTCCGCGTGGGGAGCCGCACAGCACCCCT GACCCCCACCTCCAGGGAGCAGATCCTGGCAAAGATCCGGGATTGGGGCTCAGGCTCAGACACGCTGCGC TGCCTGGCACTGGCCACCCGGGACGCGCCCCCAAGGAAGGAGGACATGGAGCTGGACGACTGCAGCAAGT TTGTGCAGTACGAGACGGACCTGACCTTCGTGGGCTGCGTAGGCATGCTGGACCCGCCGCGACCTGAGGT GGCTGCCTGCATCACACGCTGCTACCAGGCGGGCATCCGCGTGGTCATGATCACGGGGGATAACAAAGGC ACTGCCGTGGCCATCTGCCGCAGGCTTGGCATCTTTGGGGACACGGAAGACGTGGCGGGCAAGGCCTACA CGGGCCGCGAGTTTGATGACCTCAGCCCCGAGCAGCAGCGCCAGGCCTGCCGCACCGCCCGCTGCTTCGC CCGCGTGGAGCCCGCACACAAGTCCCGCATCGTGGAGAACCTGCAGTCCTTTAACGAGATCACTGCTATG ACTGGCGATGGAGTGAACGACGCACCAGCCCTGAAGAAAGCAGAGATCGGCATCGCCATGGGCTCAGGCA CGGCCGTGGCCAAGTCGGCGGCAGAGATGGTGCTGTCAGATGACAACTTTGCCTCCATCGTGGCTGCGGT GGAGGAGGGCCGGGCCATCTACAGCAACATGAAGCAATTCATCCGCTACCTCATCTCCTCCAATGTTGGC GAGGTCGTCTGCATCTTCCTCACGGCAATTCTGGGCCTGCCCGAAGCCCTGATCCCTGTGCAGCTGCTCT GGGTGAACCTGGTGACAGACGGCCTACCTGCCACGGCTCTGGGCTTCAACCCGCCAGACCTGGACATCAT GGAGAAGCTGCCCCGGAGCCCCCGAGAAGCCCTCATCAGTGGCTGGCTCTTCTTCCGATACCTGGCTATC GGAGTGTACGTAGGCCTGGCCACAGTGGCTGCCGCCACCTGGTGGTTTGTGTATGACGCCGAGGGACCTC ACATCAACTTCTACCAGCTGAGGAACTTCCTGAAGTGCTCCGAAGACAACCCGCTCTTTGCCGGCATCGA CTGTGAGGTGTTCGAGTCACGCTTCCCCACCACCATGGCCTTGTCCGTGCTCGTGACCATTGAAATGTGC AATGCCCTCAACAGCGTCTCGGAGAACCAGTCGCTGCTGCGGATGCCGCCCTGGATGAACCCCTGGCTGC TGGTGGCTGTGGCCATGTCCATGGCCCTGCACTTCCTCATCCTGCTCGTGCCGCCCCTGCCTCTCATTTT CCAGGTGACCCCACTGAGCGGGCGCCAGTGGGTGGTGGTGCTCCAGATATCTCTGCCTGTCATCCTGCTG GATGAGGCCCTCAAGTACCTGTCCCGGAACCACATGCACGCCTGTCTTTATCCAGGCCTTCTCAGGACAG TCTCGCAGGCCTGGAGTAGGCAGCCGCTGACCACCTCTTGGACCCCAGACCACACCGGGTTGGCCTCTTT GAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222876 representing NM_174956
Red=Cloning site Green=Tags(s) MEAAHLLPAADVLRHFSVTAEGGLSPAQVTGARERYGPNELPSEEGKSLWELVLEQFEDLLVRILLLAAL VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTEAIPDPRAVNQDKKNMLFS GTNITSGKAVGVAVATGLHTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS DKTGTLTTNQMSVCRMFVVAEADAGSCLLHEFTISGTTYTPEGEVRQGDQPVRCGQFDGLVELATICALC NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLQALSRVERAGACNTVIKQLMRKEFTLEFSRD RKSMSVYCTPTRPHPTGQGSKMFVKGAPESVIERCSSVRVGSRTAPLTPTSREQILAKIRDWGSGSDTLR CLALATRDAPPRKEDMELDDCSKFVQYETDLTFVGCVGMLDPPRPEVAACITRCYQAGIRVVMITGDNKG TAVAICRRLGIFGDTEDVAGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYSNMKQFIRYLISSNVG EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKLPRSPREALISGWLFFRYLAI GVYVGLATVAAATWWFVYDAEGPHINFYQLRNFLKCSEDNPLFAGIDCEVFESRFPTTMALSVLVTIEMC NALNSVSENQSLLRMPPWMNPWLLVAVAMSMALHFLILLVPPLPLIFQVTPLSGRQWVVVLQISLPVILL DEALKYLSRNHMHACLYPGLLRTVSQAWSRQPLTTSWTPDHTGLASLKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174956 |
| ORF Size | 3087 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174956.2, NP_777616.1 |
| RefSeq Size | 4840 bp |
| RefSeq ORF | 3090 bp |
| Locus ID | 489 |
| UniProt ID | Q93084 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Alzheimer's disease, Calcium signaling pathway |
| MW | 112.5 kDa |
| Gene Summary | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222876L3 | Lenti-ORF clone of ATP2A3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 3 |
CNY 12,160.00 |
|
| RC222876L4 | Lenti-ORF clone of ATP2A3 (mGFP-tagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 3 |
CNY 12,160.00 |
|
| RG222876 | ATP2A3 (tGFP-tagged) - Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 3 |
CNY 11,310.00 |
|
| SC309450 | ATP2A3 (untagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 3 |
CNY 19,860.00 |
