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USP16 (NM_006447) Human Tagged ORF Clone
CAT＃: RC222609
USP16 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 16 (USP16), transcript variant 1
ORF Plasmid: tGFP
"NM_006447" in other vectors (6)
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CNY 5,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | UBP-M; UBPM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222609 representing NM_006447
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGATTCCTCTGAAACTTTAGAACCTGTGT GCAGACACATTAGAAAAGGATTGGAACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATAT CTGCCAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAACAGAAGAAAAGCCTTCA GTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCTGTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGC ACTATCTGACGCCAAGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATGGTGTTA CGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGGGTCAAGTGGTTGATTATGTCAGAAAA CAAGCCAGCATTACAACTCCAAAGCCAGCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAAT TAGAAAAAGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAGAGAATCCTCCCAT GAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGTAATTTGGGAAACACATGTTTCTTCAATGCAGTT ATGCAGAACTTGTCACAAACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAATTG TAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATAAACCTTGAGCCTCCAGGCCCTCT TACTTTAGCCATGAGCCAGTTTCTTAATGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAA CTCTTTTCTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGACAGCCAGGAGCTGC TTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACACCAAAGAGTGAGTAAAGGAATACTTAAAGCATT TGGTAATTCTACTGAAAAGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAATCA ATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTATGATCATGTGTGATCAATGCAGAA CTGTCTCCTTGGTTCATGAATCTTTCCTTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAA AAGTGTAAATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGAGGAAGAAAAAGAT AACGACAGTTACATAAAAGAGAGAAGTGATATTCCTTCTGGAACAAGTAAGCACTTACAGAAAAAAGCAA AGAAACAAGCCAAAAAGCAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCATTT AAATGATATTTGTACTATTGACCATCCTGAAGACAGTGAATATGAAGCTGAAATGTCACTTCAAGGAGAA GTAAATATTAAATCCAACCATATTTCACAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAG ATTTGAATGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAGAGGAAGTAGATAT GAAAAATATCAACATGGATAATGATCTGGAGGTTTTAACATCTTCTCCCACTAGGAATTTAAATGGTGCC TACCTAACGGAAGGGAGCAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATGCTG CTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCATACTCCTGGAACAAAGGTGTATGA GGTTGTAAATGAAGATCCAGAAACTGCTTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAG TGTTCAATCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCGAATAAACTGCTTT GTGAAGTATGCACACGGAGACAGTGTAATGGACCAAAGGCAAATATAAAAGGTGAAAGGAAGCATGTTTA CACCAATGCCAAAAAGCAGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGATTT CAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTTTCCGGAAATCTTAGATTTGGCTC CTTTTTGCACCCTTAAATGTAAGAATGTTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGT TGTTGAACACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAGAACCGCAAATAGT CATCTCTCTAATCTTGTTCTTCACGGTGATATTCCACAAGATTTTGAAATGGAATCAAAAGGGCAGTGGT TTCACATCAGCGACACACATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCTCCT ATTTTATGAGAGAATACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222609 representing NM_006447
Red=Cloning site Green=Tags(s) MGKKRTKGKTVPIDDSSETLEPVCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKPS VWLCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVCDNEVQYCSSNQLGQVVDYVRK QASITTPKPAEKDNGNIELENKKLEKESKNEQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAV MQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTPKE LFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKS MPSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKD NDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPEDSEYEAEMSLQGE VNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGA YLTEGSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNTDE CSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRF QQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANS HLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006447 |
| ORF Size | 2469 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006447.3 |
| RefSeq Size | 2989 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 10600 |
| UniProt ID | Q9Y5T5 |
| Domains | UCH, zf-UBP |
| Protein Families | Protease |
| MW | 93.4 kDa |
| Gene Summary | This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222609L1 | Lenti ORF clone of Human ubiquitin specific peptidase 16 (USP16), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC222609L2 | Lenti ORF clone of Human ubiquitin specific peptidase 16 (USP16), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC222609L3 | Lenti ORF clone of Human ubiquitin specific peptidase 16 (USP16), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC222609L4 | Lenti ORF clone of Human ubiquitin specific peptidase 16 (USP16), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG222609 | USP16 (tGFP-tagged) - Human ubiquitin specific peptidase 16 (USP16), transcript variant 1 |
CNY 6,370.00 |
|
| SC125533 | USP16 (untagged)-Human ubiquitin specific peptidase 16 (USP16), transcript variant 1 |
CNY 2,950.00 |
