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Thyroid Peroxidase (TPO) (NM_175719) Human Tagged ORF Clone
CAT＃: RC222449
- TrueORF®
TPO (Myc-DDK-tagged)-Human thyroid peroxidase (TPO), transcript variant 2
ORF Plasmid: tGFP
"NM_175719" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MSA; TDH2A; TPX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222449 representing NM_175719
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGAGAGCGCTCGCTGTGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCT CGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGTCTCTAGCGTCTTGGAGGAAAGCAA GCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCA GCTCAGCTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAA TGGAAACATCAATACAAGCGATGAAAAGAAAAGTCAACCTGAAAACTCAACAATCACAGCATCCAACGGA TGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTGCCCCCA AAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACC CCAGATGGGGCGCCTCCAACACGGCCCTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCA GCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCCGGGAGGTGACAAGA CATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGG GACAATACATCGACCACGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGC TGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATACAACTCCCGGAGGAGGCCCGGCCG GCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCTTCGGCCGCCTGCGGCACCGGGGACCAAGGCGCGC TCTTTGGGAACCTGTCCACGGCCAACCCGCGGCAGCAGATGAACGGGTTGACCTCGTTCCTGGACGCGTC CACCGTGTATGGCAGCTCCCCGGCCCTAGAGAGGCAGCTGCGGAACTGGACCAGTGCCGAAGGGCTGCTC CGCGTCCACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCGCCACGCGCGCCTGCGG CCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTGGCCGGAGACGGCCGCGC CAGCGAGGTCCCCTCCCTGACGGCACTGCACACGCTGTGGCTGCGCGAGCACAACCGCCTGGCCGCGGCG CTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCCGTGTACCAGGAGGCGCGCAAGGTCGTGGGCGCTC TGCACCAGATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGT GGGTCCCTATGAAGGCTATGACTCCACCGCCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTC CGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACGCCAGCTTCCAGGAGCACCCCGACC TGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGAGGTTACAATGAGTG GAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCCCCGCTGACCTGAGCACAGCCATCGCCAGCAGGAGC GTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATCGATGTCTGGCTGGGAGGCTTAGCTG AAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGATGAAGGCTCT GCGGGACGGTGACTGGTTTTGGTGGGAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGGAG AAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCATGGATGCCTTCCAAG TCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGGAGGGA AACCTTTCCTCAAGACGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGACTTTGTGCACTGTGAG GAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTATGAGCTCCAAGGCCGGGAGCAGCTCA CTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGCAAAGATGTGAACGAGTGTGCAGACGGTGC CCACCCCCCCTGCCACGCCTCTGCGAGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGAC CCCTACGAGTTAGGAGACGATGGGAGAACCTGCGTAGACTCCGGGAGGCTCCCTCGGGTGACTTGGATCT CCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGGTGGAC ACGCACTGGCACTAAATCCACACTGCCCATCTCGGAGACAGGCGGAGGAACTCCCGAGCTGAGATGCGGA AAGCACCAGGCCGTAGGGACCTCACCGCAGCGGGCCGCAGCTCAGGACTCGGAGCAGGAGAGTGCTGGGA TGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCCCTC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >RC222449 representing NM_175719
Red=Cloning site Green=Tags(s) MRALAVLSVTLVMACTEAFFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSP AQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPP KCPNTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPVREVTR HVIQVSNEVVTDDDRYSDLLMAWGQYIDHDIAFTPQSTSKAAFGGGADCQMTCENQNPCFPIQLPEEARP AAGTACLPFYRSSAACGTGDQGALFGNLSTANPRQQMNGLTSFLDASTVYGSSPALERQLRNWTSAEGLL RVHARLRDSGRAYLPFVPPRAPAACAPEPGIPGETRGPCFLAGDGRASEVPSLTALHTLWLREHNRLAAA LKALNAHWSADAVYQEARKVVGALHQIITLRDYIPRILGPEAFQQYVGPYEGYDSTANPTVSNVFSTAAF RFGHATIHPLVRRLDASFQEHPDLPGLWLHQAFFSPWTLLRGGYNEWREFCGLPRLETPADLSTAIASRS VADKILDLYKHPDNIDVWLGGLAENFLPRARTGPLFACLIGKQMKALRDGDWFWWENSHVFTDAQRRELE KHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSITGMNLEAWRETFPQDDKCGFPESVENGDFVHCE ESGRRVLVYSCRHGYELQGREQLTCTQEGWDFQPPLCKDVNECADGAHPPCHASARCRNTKGGFQCLCAD PYELGDDGRTCVDSGRLPRVTWISMSLAALLIGGFAGLTSTVICRWTRTGTKSTLPISETGGGTPELRCG KHQAVGTSPQRAAAQDSEQESAGMEGRDTHRLPRAL TRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
AscI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175719 |
| ORF Size | 2628 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175719.3, NP_783650.1 |
| RefSeq Size | 2981 bp |
| RefSeq ORF | 2631 bp |
| Locus ID | 7173 |
| UniProt ID | P07202 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Metabolic pathways, Tyrosine metabolism |
| MW | 96.7 kDa |
| Gene Summary | This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222449L3 | Lenti-ORF clone of TPO (Myc-DDK-tagged)-Human thyroid peroxidase (TPO), transcript variant 2 |
CNY 9,030.00 |
|
| RC222449L4 | Lenti-ORF clone of TPO (mGFP-tagged)-Human thyroid peroxidase (TPO), transcript variant 2 |
CNY 9,030.00 |
|
| RG222449 | TPO (tGFP-tagged) - Human thyroid peroxidase (TPO), transcript variant 2 |
CNY 7,890.00 |
|
| SC307011 | TPO (untagged)-Human thyroid peroxidase (TPO), transcript variant 2 |
CNY 16,910.00 |
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