United States
Germany
Japan
United Kingdom
China
Myosin Phosphatase 2 (PPP1R12B) (NM_002481) Human Tagged Lenti ORF Clone
CAT＃: RC221975L4
- LentiORF®
Lenti-ORF clone of PPP1R12B (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1
"NM_002481" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 13,904.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | MYPT2; PP1bp55 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC221975).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002481 |
| ORF Size | 2946 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002481.3 |
| RefSeq Size | 11108 bp |
| RefSeq ORF | 2949 bp |
| Locus ID | 4660 |
| UniProt ID | O60237 |
| Domains | ANK |
| Protein Families | Druggable Genome |
| Protein Pathways | Vascular smooth muscle contraction |
| MW | 110.4 kDa |
| Gene Summary | Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221975 | PPP1R12B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1 |
CNY 11,504.00 |
|
| RC221975L1 | Lenti-ORF clone of PPP1R12B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1 |
CNY 13,904.00 |
|
| RC221975L2 | Lenti-ORF clone of PPP1R12B (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1 |
CNY 9,030.00 |
|
| RC221975L3 | Lenti-ORF clone of PPP1R12B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1 |
CNY 9,030.00 |
|
| RG221975 | PPP1R12B (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1 |
CNY 7,890.00 |
|
| SC310021 | PPP1R12B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1 |
CNY 7,220.00 |
其它Myosin Phosphatase 2产品
