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ABLIM3 (NM_014945) Human Tagged ORF Clone
CAT＃: RC221800
ABLIM3 (Myc-DDK-tagged)-Human actin binding LIM protein family, member 3 (ABLIM3)
ORF Plasmid: tGFP
"NM_014945" in other vectors (4)
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CNY 5,000.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HMFN1661 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221800 representing NM_014945
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACTAGCATTCCTTATCAGCAGAATCCTTACAATCCACGGGGCAGCTCCAATGTCATCCAGTGCT ACCGCTGTGGAGACACCTGCAAAGGGGAAGTGGTCCGCGTGCACAACAACCACTTCCACATCAGATGCTT CACCTGTCAAGTATGTGGCTGTGGCCTGGCCCAGTCAGGCTTCTTCTTCAAGAACCAGGAGTACATCTGC ACCCAGGACTACCAGCAACTCTATGGCACCCGCTGTGACAGCTGCCGGGACTTCATCACAGGCGAAGTCA TCTCGGCCCTGGGCCGCACTTACCACCCCAAGTGCTTCGTGTGCAGCTTGTGCAGGAAGCCTTTCCCCAT TGGAGACAAGGTGACCTTCAGCGGTAAAGAATGTGTGTGCCAAACGTGCTCCCAGTCCATGGCCAGCAGT AAGCCCATCAAGATTCGTGGACCAAGCCACTGTGCCGGGTGCAAGGAGGAGATCAAGCACGGCCAGTCAC TCCTGGCTCTGGACAAGCAGTGGCACGTCAGCTGCTTCAAGTGCCAGACCTGCAGCGTCATCCTCACCGG GGAGTATATCAGCAAGGATGGTGTTCCATACTGTGAGTCCGACTACCATGCCCAGTTTGGCATTAAATGT GAGACTTGTGACCGATACATCAGTGGCAGAGTCTTGGAGGCAGGAGGGAAGCACTACCACCCAACCTGTG CCAGGTGTGTACGCTGCCACCAGATGTTCACCGAAGGAGAGGAAATGTACCTCACAGGTTCCGAGGTTTG GCACCCCATCTGCAAACAGGCAGCCCGGGCAGAGAAGAAGTTAAAGCATAGACGGACATCTGAAACCTCC ATCTCACCCCCTGGATCCAGCATTGGGTCACCCAACCGAGTCATCTGCGCTAAAGTGGATAATGAGATCC TTAATTACAAAGACCTGGCGGCTCTCCCCAAGGTTAAGTCTATCTACGAGGTACAACGCCCCGACCTCAT TTCCTATGAGCCTCATTCCAGATACATGTCCGACGAGATGCTGGAGAGATGTGGCTATGGAGAGTCGCTG GGAACATTATCTCCCTACTCCCAGGACATCTACGAGAACCTGGACCTCCGGCAGAGACGGGCCTCCAGCC CGGGGTACATAGACTCCCCCACCTACAGCCGGCAGGGCATGTCCCCCACCTTCTCCCGCTCACCTCACCA CTACTACCGCTCTGGGCCCGAGAGTGGCCGGAGCTCTCCATACCATAGCCAGTTAGATGTGAGGTCCTCC ACTCCAACCTCTTACCAGGCTCCCAAGCACTTTCACATCCCAGCTGGAGACAGTAACATCTACCGGAAAC CCCCGATCTACAAACGGCATGGTGATTTGTCTACAGCAACCAAGAGCAAAACAAGTGAAGACATCAGCCA GACCTCCAAGTACAGTCCCATCTACTCGCCAGACCCCTACTATGCTTCGGAGTCTGAGTACTGGACCTAC CATGGGTCCCCCAAAGTGCCCCGAGCCAGAAGGTTCTCGTCTGGAGGAGAGGAGGATGATTTTGACCGCA GCATGCACAAGCTCCAAAGTGGAATTGGCCGGCTGATTCTGAAGGAAGAAATGAAGGCCCGGTCGAGCTC CTATGCAGATCCCTGGACCCCTCCCCGGAGCTCCACCAGCAGCCGGGAAGCCCTGCACACAGCTGGCTAT GAGATGTCCCTCAATGGCTCCCCTCGGTCGCACTACCTGGCTGACAGTGATCCTCTCATCTCCAAATCTG CCTCCCTGCCTGCCTACCGAAGAAATGGGCTGCACAGGACACCCAGCGCAGACCTCTTCCACTACGACAG CATGAACGCAGTCAACTGGGGCATGCGAGAGTACAAGATCTACCCTTATGAACTGCTGCTGGTGACTACA AGAGGAAGAAACCGACTGCCCAAGGATGTAGACAGGACCCGTTTAGAGCGCCACCTGTCCCAGGAAGAGT TCTACCAAGTCTTTGGCATGACCATCTCTGAGTTTGACCGGCTGGCCCTCTGGAAGAGGAATGAACTGAA GAAGCAAGCCCGGCTGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221800 representing NM_014945
Red=Cloning site Green=Tags(s) MNTSIPYQQNPYNPRGSSNVIQCYRCGDTCKGEVVRVHNNHFHIRCFTCQVCGCGLAQSGFFFKNQEYIC TQDYQQLYGTRCDSCRDFITGEVISALGRTYHPKCFVCSLCRKPFPIGDKVTFSGKECVCQTCSQSMASS KPIKIRGPSHCAGCKEEIKHGQSLLALDKQWHVSCFKCQTCSVILTGEYISKDGVPYCESDYHAQFGIKC ETCDRYISGRVLEAGGKHYHPTCARCVRCHQMFTEGEEMYLTGSEVWHPICKQAARAEKKLKHRRTSETS ISPPGSSIGSPNRVICAKVDNEILNYKDLAALPKVKSIYEVQRPDLISYEPHSRYMSDEMLERCGYGESL GTLSPYSQDIYENLDLRQRRASSPGYIDSPTYSRQGMSPTFSRSPHHYYRSGPESGRSSPYHSQLDVRSS TPTSYQAPKHFHIPAGDSNIYRKPPIYKRHGDLSTATKSKTSEDISQTSKYSPIYSPDPYYASESEYWTY HGSPKVPRARRFSSGGEEDDFDRSMHKLQSGIGRLILKEEMKARSSSYADPWTPPRSSTSSREALHTAGY EMSLNGSPRSHYLADSDPLISKSASLPAYRRNGLHRTPSADLFHYDSMNAVNWGMREYKIYPYELLLVTT RGRNRLPKDVDRTRLERHLSQEEFYQVFGMTISEFDRLALWKRNELKKQARLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014945 |
| ORF Size | 2049 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014945.5 |
| RefSeq Size | 4335 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 22885 |
| UniProt ID | O94929 |
| Domains | LIM, VHP |
| Protein Pathways | Axon guidance |
| MW | 78.3 kDa |
| Gene Summary | This gene encodes a member of the actin-binding LIM (abLIM) family of proteins. These proteins are characterized by an N-terminal LIM domain and a C-terminal dematin-like domain. The encoded protein interacts with actin filaments and may be a component of adherens junctions in several cell types. A variant of this gene may be associated with pain sensitivity in male human patients. [provided by RefSeq, Sep 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221800L3 | Lenti-ORF clone of ABLIM3 (Myc-DDK-tagged)-Human actin binding LIM protein family, member 3 (ABLIM3) |
CNY 7,700.00 |
|
| RC221800L4 | Lenti-ORF clone of ABLIM3 (mGFP-tagged)-Human actin binding LIM protein family, member 3 (ABLIM3) |
CNY 7,700.00 |
|
| RG221800 | ABLIM3 (tGFP-tagged) - Human actin binding LIM protein family, member 3 (ABLIM3) |
CNY 6,370.00 |
|
| SC114724 | ABLIM3 (untagged)-Human actin binding LIM protein family, member 3 (ABLIM3) |
CNY 5,008.00 |
