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PPP1R10 (NM_002714) Human Tagged ORF Clone
CAT＃: RC221507
PPP1R10 (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10)
ORF Plasmid: tGFP
"NM_002714" in other vectors (6)
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CNY 6,888.00
CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAT53; FB19; p99; PNUTS; PP1R10; R111 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221507 representing NM_002714
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCGGGTCCCATAGACCCCAAAGAACTTCTCAAGGGCCTGGACAGCTTCCTTAACCGAGATGGGG AAGTCAAAAGTGTGGATGGGATTTCCAAGATCTTCAGTTTGATGAAGGAAGCACGAAAGATGGTGAGTCG ATGCACTTACTTGAACATTCTCCTGCAGACCCGTTCACCAGAAATATTGGTCAAATTTATTGACGTTGGC GGCTACAAACTTCTTAACAATTGGCTGACGTATTCAAAGACAACCAACAACATTCCCCTCCTCCAGCAAA TTCTACTGACCCTGCAGCATCTACCGCTCACTGTAGACCATCTCAAGCAGAACAACACAGCTAAACTGGT GAAGCAGCTGAGCAAGTCAAGTGAGGATGAAGAGCTCCGGAAATTGGCCTCAGTCCTTGTCAGCGACTGG ATGGCTGTCATCCGCTCTCAGAGCAGTACCCAGCCTGCTGAGAAAGATAAGAAGAAACGTAAAGATGAAG GAAAAAGTCGAACTACCCTTCCTGAGCGACCTTTGACAGAGGTGAAGGCTGAGACCCGGGCTGAGGAGGC CCCAGAGAAGAAGAGGGAGAAGCCCAAGTCTCTTCGCACCACAGCACCCAGTCATGCCAAGTTCCGTTCC ACTGGACTAGAGCTGGAGACACCATCCTTGGTGCCTGTGAAGAAGAATGCCAGCACAGTGGTGGTTTCTG ACAAGTACAACCTTAAACCCATCCCCCTCAAACGTCAGAGCAACGTAGCTGCTCCAGGAGATGCCACTCC CCCTGCAGAGAAGAAATACAAGCCACTCAACACAACACCTAATGCCACCAAAGAGATCAAAGTGAAGATC ATCCCGCCACAGCCTATGGAGGGCCTGGGCTTTCTGGATGCTCTTAATTCAGCCCCTGTTCCAGGCATCA AAATTAAGAAGAAAAAAAAAGTACTGTCACCTACGGCTGCCAAGCCAAGCCCCTTTGAAGGGAAAACGAG CACAGAACCAAGCACAGCCAAACCTTCTTCCCCAGAACCAGCACCACCTTCTGAGGCAATGGACGCAGAC CGTCCAGGCACCCCGGTTCCCCCTGTTGAAGTCCCGGAGCTCATGGATACAGCCTCTTTGGAGCCAGGAG CTCTGGATGCCAAGCCAGTGGAGAGTCCTGGAGATCCTAACCAACTGACCCGGAAAGGCAGGAAGAGGAA AAGTGTGACATGGCCTGAGGAAGGCAAACTGAGAGAATATTTCTATTTTGAATTGGATGAAACTGAACGA GTAAATGTGAATAAGATCAAGGACTTTGGTGAGGCGGCTAAGCGAGAGATACTGTCAGACCGACATGCAT TTGAGACAGCGCGGCGTCTGAGCCATGATAACATGGAGGAGAAGGTGCCCTGGGTGTGCCCCCGGCCCCT GGTTCTGCCCTCACCTCTTGTCACCCCTGGAAGCAATAGTCAGGAGCGATATATCCAGGCTGAGCGGGAG AAGGGAATCCTTCAGGAGCTCTTCCTGAACAAGGAGAGTCCTCATGAGCCTGATCCTGAGCCCTACGAGC CCATACCCCCTAAACTCATCCCCCTAGATGAGGAGTGTTCCATGGATGAGACTCCGTATGTTGAGACTCT GGAACCTGGGGGGTCAGGTGGCTCACCTGATGGGGCAGGAGGCTCCAAGTTGCCTCCAGTTCTGGCCAAT CTTATGGGAAGCATGGGTGCTGGAAAGGGCCCCCAAGGCCCTGGAGGAGGAGGCATTAATGTCCAAGAGA TCCTCACCTCCATCATGGGTAGCCCAAACAGTCATCCTTCAGAGGAACTACTGAAACAACCAGACTATTC GGACAAGATCAAGCAGATGCTGGTGCCACATGGACTCCTAGGCCCTGGCCCAATAGCCAATGGTTTCCCA CCAGGGGGTCCTGGGGGCCCCAAGGGCATGCAGCACTTTCCCCCTGGACCTGGGGGACCTATGCCAGGTC CCCATGGAGGCCCTGGTGGGCCAGTGGGTCCACGTCTTCTGGGTCCTCCACCCCCTCCCCGGGGAGGTGA TCCCTTCTGGGATGGCCCGGGCGACCCTATGCGGGGTGGCCCAATGCGGGGGGGTCCAGGACCAGGTCCT GGACCATACCATAGAGGCCGAGGTGGCCGAGGAGGAAACGAACCTCCTCCTCCTCCTCCTCCATTCCGAG GCGCCAGAGGAGGTCGCTCTGGAGGAGGACCCCCAAATGGACGAGGGGGCCCTGGTGGGGGCATGGTTGG AGGTGGTGGGCATCGTCCTCACGAAGGCCCTGGTGGGGGCATGGGCAACAGCAGTGGACATCGTCCCCAC GAAGGCCCTGGCGGTGGCATGGGAAGTGGGCATCGCCCCCATGAAGGCCCTGGTGGTAGCATGGGTGGGG GTGGAGGACATCGTCCCCACGAAGGCCCTGGCGGTGGCATCAGTGGTGGCAGTGGCCATCGTCCCCATGA AGGCCCTGGCGGAGGAATGGGTGCCGGTGGTGGACATCGCCCCCACGAAGGCCCTGGCGGAAGCATGGGT GGAAGTGGTGGACATCGTCCCCATGAAGGCCCTGGACACGGGGGGCCCCATGGCCACCGGCCTCATGATG TCCCTGGTCACCGAGGCCATGACCATCGAGGGCCGCCACCTCATGAGCACCGTGGCCATGATGGTCCTGG CCACGGGGGAGGGGGCCACCGAGGGCACGATGGAGGCCACAGCCATGGAGGAGACATGTCAAACCGCCCT GTCTGCCGACATTTCATGATGAAGGGCAACTGCCGCTATGAGAACAACTGTGCCTTCTACCACCCGGGTG TCAATGGGCCCCCCCTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221507 representing NM_002714
Red=Cloning site Green=Tags(s) MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVG GYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDW MAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRS TGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKI IPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEPSTAKPSSPEPAPPSEAMDAD RPGTPVPPVEVPELMDTASLEPGALDAKPVESPGDPNQLTRKGRKRKSVTWPEEGKLREYFYFELDETER VNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVTPGSNSQERYIQAERE KGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECSMDETPYVETLEPGGSGGSPDGAGGSKLPPVLAN LMGSMGAGKGPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKIKQMLVPHGLLGPGPIANGFP PGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPPRGGDPFWDGPGDPMRGGPMRGGPGPGP GPYHRGRGGRGGNEPPPPPPPFRGARGGRSGGGPPNGRGGPGGGMVGGGGHRPHEGPGGGMGNSSGHRPH EGPGGGMGSGHRPHEGPGGSMGGGGGHRPHEGPGGGISGGSGHRPHEGPGGGMGAGGGHRPHEGPGGSMG GSGGHRPHEGPGHGGPHGHRPHDVPGHRGHDHRGPPPHEHRGHDGPGHGGGGHRGHDGGHSHGGDMSNRP VCRHFMMKGNCRYENNCAFYHPGVNGPPLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002714 |
| ORF Size | 2820 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002714.4 |
| RefSeq Size | 4540 bp |
| RefSeq ORF | 2823 bp |
| Locus ID | 5514 |
| UniProt ID | Q96QC0 |
| Domains | zf-CCCH, TFS2N |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| MW | 98.9 kDa |
| Gene Summary | This gene encodes a protein phosphatase 1 binding protein. The encoded protein plays a role in many cellular processes including cell cycle progression, DNA repair and apoptosis by regulating the activity of protein phosphatase 1. This gene lies within the major histocompatibility complex class I region on chromosome 6, and alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Combination therapy with the CDK7 inhibitor and the tyrosine kinase inhibitor exerts synergistic anticancer effects against MYCN-amplified neuroblastoma
,Tee, AE;Ciampa, OC;Wong, M;Fletcher, JI;Kamili, A;Chen, J;Ho, N;Sun, Y;Carter, DR;Cheung, BB;Marshall, GM;Liu, PY;Liu, T;,
Int. J. Cancer
,PubMed ID 32086952
[PPP1R10]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221507L1 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221507L2 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10), mGFP tagged |
CNY 9,030.00 |
|
| RC221507L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221507L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10), mGFP tagged |
CNY 9,030.00 |
|
| RG221507 | PPP1R10 (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10) |
CNY 7,890.00 |
|
| SC118435 | PPP1R10 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10) |
CNY 6,896.00 |
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